Restriction map for
pFN21AE6232
(Circular) (Six-base) MAPSORT of: pFN21AE6232.seq Check: 8142 from: 1 to: 5605
pFN21AE6232 5605 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 3668 5052 5320 1632
Size: 542 1494 1384 268 1917
Fragments arranged by size:
1917 1494 1384 542 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2768 3348 4920 2768
Size: 580 1572 3453
Fragments arranged by size:
3453 1572 580
ApaI G_GGCC'C
Cuts at: 2062 2062
Size: 5605
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2879 2914 3140 3292 5401 1493
Size: 1386 35 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2442 2930 1870
Size: 73 499 488 4545
Fragments arranged by size:
4545 499 488 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2733 2924 1391
Size: 671 671 191 4072
Fragments arranged by size:
4072 671 671 191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4216 136
Size: 107 122 71 631 605 337 2207 1525
Fragments arranged by size:
2207 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 2304 3022 2304
Size: 718 4887
Bme1580I G_kGCm'C
Cuts at: 1790 2062 3666 4822 1790
Size: 272 1604 1156 2573
Fragments arranged by size:
2573 1604 1156 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2140 2369 2790 4265 1239
Size: 859 42 229 421 1475 2579
Fragments arranged by size:
2579 1475 859 421 229 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2101 2688 4268 882
Size: 617 602 587 1580 2219
Fragments arranged by size:
2219 1580 617 602 587
BsaAI yAC'GTr
Cuts at: 493 1204 2888 3396 493
Size: 711 1684 508 2702
Fragments arranged by size:
2702 1684 711 508
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2195 2452 2455 1186
Size: 909 100 257 3 4336
Fragments arranged by size:
4336 909 257 100 3
BseYI C'CCAG_C
Cuts at: 2029 2050 4808 5304 2029
Size: 21 2758 496 2330
Fragments arranged by size:
2758 2330 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2491 1163
Size: 573 755 4277
Fragments arranged by size:
4277 755 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2762 3666 3751 4822 2762
Size: 904 85 1071 3545
Fragments arranged by size:
3545 1071 904 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2733 2762 2924 3666 3751 4822
Size: 399 272 671 29 162 742 85 1071
Cuts at: 4822 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 671 399 272 162 85
29
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2960 844
Size: 1389 727 3489
Fragments arranged by size:
3489 1389 727
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 2299
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600
Size: 963 367 385 17 1452 86 12 346
Cuts at: 5600 1972
Size: 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2090 2090
Size: 5605
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3700
Fragments arranged by size:
3700 939 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3121 3915 3935 2503
Size: 618 794 20 4173
Fragments arranged by size:
4173 794 618 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2900 2900
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2519 4612 809
Size: 507 1203 2093 1802
Fragments arranged by size:
2093 1802 1203 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2709 3052
Size: 54 1425 50 275 18 234 645 343
Cuts at: 3052 3941 5570 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 645 343 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2140 2174 2369 2790 3668 4265
Size: 393 466 42 34 195 421 878 597
Cuts at: 4265 5052 5320 1239
Size: 787 268 1524
Fragments arranged by size:
1524 878 787 597 466 421 393 268
195 42 34
EcoO109I rG'GnC_Cy
Cuts at: 2059 2197 2481 2774 2059
Size: 138 284 293 4890
Fragments arranged by size:
4890 293 284 138
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2073 4752 1678
Size: 395 2679 2531
Fragments arranged by size:
2679 2531 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2511 2543 4246
Size: 32 135 32 701 32 206 32 1703
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1703 701 206 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2234 2265 4719 4903 1305
Size: 929 31 2454 184 2007
Fragments arranged by size:
2454 2007 929 184 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 2904
Fragments arranged by size:
2904 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2618 3563 3922 4081 518
Size: 648 1047 405 945 359 159 2042
Fragments arranged by size:
2042 1047 945 648 405 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3021 3698 4846 5091 1606
Size: 423 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3700
Fragments arranged by size:
3700 939 552 414
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2108 1348
Size: 760 4845
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2433 2433
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 2564 3674 3706 5187 5219 2532
Size: 32 1110 32 1481 32 2918
Fragments arranged by size:
2918 1481 1110 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2774 2197
Size: 577 5028
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspOMI G'GGCC_C
Cuts at: 2058 2058
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2149 2823 2854 2957 830
Size: 1319 674 31 103 3478
Fragments arranged by size:
3478 1319 674 103 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2029 2029
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SexAI A'CCwGG_T
Cuts at: 2834 2896 2834
Size: 62 5543
SfcI C'TryA_G
Cuts at: 826 1045 2145 2819 2850 2953 4088 4769 4960
Size: 219 1100 674 31 103 1135 681 191
Cuts at: 4960 826
Size: 1471
Fragments arranged by size:
1471 1135 1100 681 674 219 191 103
31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2444 2932 1872
Size: 572 488 4545
Fragments arranged by size:
4545 572 488
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 2418 3044 513
Size: 552 939 387 27 626 3074
Fragments arranged by size:
3074 939 626 552 387 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3851 5563
Size: 274 80 33 51 1443 846 1028 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1443 1028 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2884 2916 5565 5597 2884
Size: 32 2649 32 2892
Fragments arranged by size:
2892 2649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 1316
Size: 1113 4492
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2115 2011
Size: 104 5501
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2442 2930 1870
Size: 572 488 4545
Fragments arranged by size:
4545 572 488
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI