Restriction map for
pFN21AE6221
(Circular) (Six-base) MAPSORT of: pFN21AE6221.seq Check: 9306 from: 1 to: 5608
pFN21AE6221 5608 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 2227 1166
Size: 1061 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaI G_GGCC'C
Cuts at: 2099 2246 2099
Size: 147 5461
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2660 2917 3143 3295 5404 1493
Size: 1167 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1167 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2167 2273 2933 1870
Size: 73 141 83 106 660 4545
Fragments arranged by size:
4545 660 141 106 83 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2929 4384 618
Size: 325 217 627 385 757 1455 1842
Fragments arranged by size:
1842 1455 757 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 2099 2246 2927 1391
Size: 708 147 681 4072
Fragments arranged by size:
4072 708 681 147
BbeI G_GCGC'C
Cuts at: 2176 2176
Size: 5608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2772 928
Size: 971 873 3764
Fragments arranged by size:
3764 971 873
BbvCI CC'TCA_GC
Cuts at: 2126 2204 2126
Size: 78 5530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2093 2104 2127 2138 3799 3833 1903
Size: 34 156 11 23 11 1661 34 3678
Fragments arranged by size:
3678 1661 156 34 34 23 11 11
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2587 5603 2587
Size: 3016 2592
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2246 3669 4825 1790
Size: 309 147 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 309 147
BmgBI CAC'GTC
Cuts at: 1318 2347 1318
Size: 1029 4579
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 3339 4297
Size: 416 53 83 399 24 1034 888 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1034 958 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4268 1239
Size: 3029 2579
Bpu10I CC'TnA_GC
Cuts at: 2126 2204 2126
Size: 78 5530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2173 3415 3797 4432
Size: 53 83 186 936 640 1242 382 635
Cuts at: 4432 275
Size: 1451
Fragments arranged by size:
1451 1242 936 640 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2334 1186
Size: 1148 4460
BseYI C'CCAG_C
Cuts at: 2098 4811 5307 2098
Size: 2713 496 2399
Fragments arranged by size:
2713 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2246 2927 3669 3754 4825 1391
Size: 399 309 147 681 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 399 309 147 85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 844
Size: 2119 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 4103 4277 65
Size: 1183 1258 1597 174 1396
Fragments arranged by size:
1597 1396 1258 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 2139 1925
Size: 214 5394
BssSI C'ACGA_G
Cuts at: 2692 3668 4680 2692
Size: 976 1012 3620
Fragments arranged by size:
3620 1012 976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2229 2229
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2587 2938 3305 3690 3707 5159 5245 5257
Size: 615 351 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 615 385 367 351 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2228 513
Size: 552 939 224 3893
Fragments arranged by size:
3893 939 552 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 2626 487
Size: 683 56 798 602 3469
Fragments arranged by size:
3469 798 683 602 56
BtsI GCAGTG_nn'
Cuts at: 2208 3124 3918 3938 2208
Size: 916 794 20 3878
Fragments arranged by size:
3878 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2715 2836
Size: 54 1425 50 275 18 223 662 121
Cuts at: 2836 3055 3944 5573 8
Size: 219 889 1629 43
Fragments arranged by size:
1629 1425 889 662 275 223 219 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2694 3538 1353
Size: 1341 844 3423
Fragments arranged by size:
3423 1341 844
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3671 4268 5055 5323 1239
Size: 393 2039 597 787 268 1524
Fragments arranged by size:
2039 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2242 2243 2095
Size: 147 1 5460
Fragments arranged by size:
5460 147 1
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2068 2176 4755 1678
Size: 390 108 2579 2531
Fragments arranged by size:
2579 2531 390 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2688 2720 4249 4281 4323
Size: 32 135 32 1116 32 1529 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 1529 1116 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KasI G'GCGC_C
Cuts at: 2172 2172
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 2871 3207 2871
Size: 336 5272
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 2717 2838 10
Size: 54 1425 343 223 662 121 2780
Fragments arranged by size:
2780 1425 662 343 223 121 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2227 2882 3566 3925 4084 518
Size: 648 843 218 655 684 359 159 2042
Fragments arranged by size:
2042 843 684 655 648 359 218 159
MspA1I CmG'CkG
Cuts at: 1606 3024 3701 4849 5094 1606
Size: 1418 677 1148 245 2120
Fragments arranged by size:
2120 1418 1148 677 245
NarI GG'CG_CC
Cuts at: 2173 2173
Size: 5608
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2285 2285
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2095 2242 2095
Size: 147 5461
PspXI vC'TCGA_Gb
Cuts at: 2084 2084
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2956 4091 4772 4963 826
Size: 219 1911 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2174 2174
Size: 5608
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2275 2935 1872
Size: 403 660 4545
Fragments arranged by size:
4545 660 403
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 3712 4613 4875 820
Size: 197 926 141 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SrfI GCCC'GGGC
Cuts at: 2275 2275
Size: 5608
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 2751 3047 513
Size: 552 939 747 296 3074
Fragments arranged by size:
3074 939 747 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 3635 3652 3805 3990 4435 325
Size: 1255 459 1596 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 459 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 3854 5566
Size: 274 80 33 51 1443 819 1058 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1712 1443 1058 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2824 2944 2824
Size: 120 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 2084 1943
Size: 141 5467
XmaI C'CCGG_G
Cuts at: 1870 2273 2933 1870
Size: 403 660 4545
Fragments arranged by size:
4545 660 403
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SwaI