Restriction map for
pFN21AE6218
(Circular) (Six-base) MAPSORT of: pFN21AE6218.seq Check: 1379 from: 1 to: 5575
pFN21AE6218 5575 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2672 2672
Size: 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 2918 1937
Size: 133 848 4594
Fragments arranged by size:
4594 848 133
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2645 2837 3318 4890 2645
Size: 192 481 1572 3330
Fragments arranged by size:
3330 1572 481 192
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2255 2417 2884 3110 3262 5371 1493
Size: 762 162 467 226 152 2109 1697
Fragments arranged by size:
2109 1697 762 467 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 1870
Size: 73 957 4545
Fragments arranged by size:
4545 957 73
BamHI G'GATC_C
Cuts at: 2750 2905 3272 2750
Size: 155 367 5053
Fragments arranged by size:
5053 367 155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 4351 618
Size: 325 217 627 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2894 1391
Size: 1503 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2776 928
Size: 971 877 3727
Fragments arranged by size:
3727 971 877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2790 5570 2790
Size: 2780 2795
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2519 3636 4792 1790
Size: 729 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 729
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4235 1239
Size: 2996 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2604 4238 882
Size: 617 1105 1634 2219
Fragments arranged by size:
2219 1634 1105 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2034 4008 4406 4680 4827 5238 2034
Size: 1974 398 274 147 411 2371
Fragments arranged by size:
2371 1974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5350
Fragments arranged by size:
5350 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4644
BseYI C'CCAG_C
Cuts at: 2837 4778 5274 2837
Size: 1941 496 3138
Fragments arranged by size:
3138 1941 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 3636 3721 4792 2042
Size: 1594 85 1071 2825
Fragments arranged by size:
2825 1594 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2519 2894 3636 3721 4792 1391
Size: 399 252 477 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 477 399 375 252 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 2130 2181 3464 5194 2130
Size: 51 1283 1730 2511
Fragments arranged by size:
2511 1730 1283 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2672 2930 844
Size: 1828 258 3489
Fragments arranged by size:
3489 1828 258
BsrBI CCG'CTC
Cuts at: 1394 1823 2510 3462 1394
Size: 429 687 952 3507
Fragments arranged by size:
3507 952 687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2750 2790 2905 3272 3657 3674 5126 5212
Size: 778 40 115 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 778 385 367 346 115 86
40 17 12
BstZ17I GTA'TAC
Cuts at: 2071 2071
Size: 5575
Bsu36I CC'TnA_GG
Cuts at: 2013 2213 2013
Size: 200 5375
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5023
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2607 2642 529
Size: 35 2043 35 3462
Fragments arranged by size:
3462 2043 35 35
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 3022 3911
Size: 54 1425 50 275 18 324 868 889
Cuts at: 3911 5540 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 868 324 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3505 1353
Size: 1103 1049 3423
Fragments arranged by size:
3423 1103 1049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 4162 4536 4682 387
Size: 1064 138 521 2052 374 146 1280
Fragments arranged by size:
2052 1280 1064 521 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3638 4235 5022 5290 1239
Size: 393 2006 597 787 268 1524
Fragments arranged by size:
2006 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5575
EcoO109I rG'GnC_Cy
Cuts at: 2614 2614
Size: 5575
EcoRI G'AATT_C
Cuts at: 2255 2884 3262 2255
Size: 629 378 4568
Fragments arranged by size:
4568 629 378
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2616 2648 4216 4248 4290
Size: 32 135 32 1044 32 1568 32 42
Cuts at: 4290 4322 1373
Size: 32 2626
Fragments arranged by size:
2626 1568 1044 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 3920
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 2193 3174 2193
Size: 981 4594
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 4689 4873 1305
Size: 1257 2127 184 2007
Fragments arranged by size:
2127 2007 1257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 10
Size: 54 1425 343 324 3429
Fragments arranged by size:
3429 1425 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2991 3668 4816 5061 1606
Size: 1385 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5023
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 2381 3145 2381
Size: 764 4811
PstI C_TGCA'G
Cuts at: 830 2461 2669 2927 830
Size: 1631 208 258 3478
Fragments arranged by size:
3478 1631 258 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SapI GCTCTTCn'nnn_
Cuts at: 2456 2456
Size: 5575
SbfI CC_TGCA'GG
Cuts at: 2669 2927 2669
Size: 258 5317
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SexAI A'CCwGG_T
Cuts at: 2296 2296
Size: 5575
SfcI C'TryA_G
Cuts at: 826 1045 2457 2535 2665 2763 2923 4058 4739
Size: 219 1412 78 130 98 160 1135 681
Cuts at: 4739 4930 826
Size: 191 1471
Fragments arranged by size:
1471 1412 1135 681 219 191 160 130
98 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 3014 513
Size: 552 1949 3074
Fragments arranged by size:
3074 1949 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3602 3619 3772 3957 4402 325
Size: 1255 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2310 3821
Size: 274 80 33 51 1443 45 288 1511
Cuts at: 3821 5533 96
Size: 1712 138
Fragments arranged by size:
1712 1511 1443 288 274 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 2783 3704 2783
Size: 921 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI XcmI