Restriction map for
pFN21AE6214
(Circular) (Six-base) MAPSORT of: pFN21AE6214.seq Check: 2994 from: 1 to: 5518
pFN21AE6214 5518 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2464 2464
Size: 5518
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AfeI AGC'GCT
Cuts at: 2549 2549
Size: 5518
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 1184
Size: 1089 4429
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2236 2827 3053 3205 5314 1493
Size: 743 591 226 152 2109 1697
Fragments arranged by size:
2109 1697 743 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2843 1870
Size: 73 113 787 4545
Fragments arranged by size:
4545 787 113 73
BamHI G'GATC_C
Cuts at: 2757 2848 3215 2757
Size: 91 367 5060
Fragments arranged by size:
5060 367 91
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 2839 4294 618
Size: 325 217 627 893 159 1455 1842
Fragments arranged by size:
1842 1455 893 627 325 217 159
BanII G_rGCy'C
Cuts at: 1391 2165 2330 2711 2837 1391
Size: 774 165 381 126 4072
Fragments arranged by size:
4072 774 381 165 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2579 2613 3709 3743 1903
Size: 34 642 34 1096 34 3678
Fragments arranged by size:
3678 1096 642 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2145 3579 4735 1790
Size: 355 1434 1156 2573
Fragments arranged by size:
2573 1434 1156 355
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 4522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2344 2365 2564 4178 1239
Size: 1105 21 199 1614 2579
Fragments arranged by size:
2579 1614 1105 199 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2016 4181 882
Size: 617 517 2165 2219
Fragments arranged by size:
2219 2165 617 517
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3309 493
Size: 711 1263 842 2702
Fragments arranged by size:
2702 1263 842 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 3325 3707 4342
Size: 53 83 186 936 547 1245 382 635
Cuts at: 4342 275
Size: 1451
Fragments arranged by size:
1451 1245 936 635 547 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2428 2732 3951 4349 4623 4770 5181 2428
Size: 304 1219 398 274 147 411 2765
Fragments arranged by size:
2765 1219 411 398 304 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2753 1186
Size: 1567 3951
BseYI C'CCAG_C
Cuts at: 2074 2226 2370 4721 5217 2074
Size: 152 144 2351 496 2375
Fragments arranged by size:
2375 2351 496 152 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 2219 1163
Size: 573 465 18 4462
Fragments arranged by size:
4462 573 465 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2968 3729 4757 1540
Size: 275 186 48 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2330 3579 3664 4735 2330
Size: 1249 85 1071 3113
Fragments arranged by size:
3113 1249 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2165 2330 2711 2837 3579 3664
Size: 399 355 20 165 381 126 742 85
Cuts at: 3664 4735 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 381 355 165 126
85 20
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 2464 2873 844
Size: 1516 104 409 3489
Fragments arranged by size:
3489 1516 409 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2555 4162 4349 1700
Size: 110 745 1607 187 2869
Fragments arranged by size:
2869 1607 745 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 2070 2639 1925
Size: 145 569 4804
Fragments arranged by size:
4804 569 145
BssSI C'ACGA_G
Cuts at: 2345 3578 4590 2345
Size: 1233 1012 3273
Fragments arranged by size:
3273 1233 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 2500 1184
Size: 1316 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 2725
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2563 2757 2848 3215 3600 3617 5069 5155
Size: 591 194 91 367 385 17 1452 86
Cuts at: 5155 5167 5513 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 591 385 367 346 194 91
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2507 2507
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2725 487
Size: 683 56 1499 3280
Fragments arranged by size:
3280 1499 683 56
BtsI GCAGTG_nn'
Cuts at: 2338 3034 3828 3848 2338
Size: 696 794 20 4008
Fragments arranged by size:
4008 794 696 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2568 2965
Size: 54 1425 50 275 18 216 522 397
Cuts at: 2965 3854 5483 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 522 397 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2965 1537
Size: 275 234 919 4090
Fragments arranged by size:
4090 919 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2328 2328
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2344 2365 2564 3581 4178 4965 5233
Size: 393 712 21 199 1017 597 787 268
Cuts at: 5233 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 712 597 393 268 199
21
EcoO109I rG'GnC_Cy
Cuts at: 2133 2319 2133
Size: 186 5332
EcoRI G'AATT_C
Cuts at: 2236 2827 3205 2236
Size: 591 378 4549
Fragments arranged by size:
4549 591 378
FspI TGC'GCA
Cuts at: 2156 2356 4024 2156
Size: 200 1668 3650
Fragments arranged by size:
3650 1668 200
FspAI rTGC'GCAy
Cuts at: 2156 2156
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2551 2778 4665 1678
Size: 873 227 1887 2531
Fragments arranged by size:
2531 1887 873 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373
Size: 32 135 32 2587 32 42 32 2626
Fragments arranged by size:
2626 2587 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 2206 3117 2206
Size: 911 4607
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 2958
Fragments arranged by size:
2958 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2257 2305 2934 3611 4759 5004 1606
Size: 614 37 48 629 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 629 614 245 48 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2046 2965 2046
Size: 919 4599
NspI r_CATG'y
Cuts at: 1132 1229 2876 4421 1132
Size: 97 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PfoI T'CCnGG_A
Cuts at: 2129 2381 2129
Size: 252 5266
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PmlI CAC'GTG
Cuts at: 2467 2467
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2133 2133
Size: 5518
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2220 2305 2220
Size: 85 5433
SacI G_AGCT'C
Cuts at: 2330 2330
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2058 2845 1872
Size: 186 787 4545
Fragments arranged by size:
4545 787 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 2957 513
Size: 552 939 102 851 3074
Fragments arranged by size:
3074 939 851 552 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2281 2698 3545 3562 3715 3900
Size: 1255 560 141 417 847 17 153 185
Cuts at: 3900 4345 325
Size: 445 1498
Fragments arranged by size:
1498 1255 847 560 445 417 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2056 2843 1870
Size: 186 787 4545
Fragments arranged by size:
4545 787 186
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI