Restriction map for
pFN21AE6211
(Circular) (Six-base) MAPSORT of: pFN21AE6211.seq Check: 8624 from: 1 to: 5308
pFN21AE6211 5308 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2439 3118 278
Size: 53 83 186 936 903 679 2468
Fragments arranged by size:
2468 936 903 679 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 1937
Size: 714 4594
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AfeI AGC'GCT
Cuts at: 2241 2241
Size: 5308
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 4207 5184 1203
Size: 3004 977 1327
Fragments arranged by size:
3004 1327 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 2262
Size: 32 5276
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 1870
Size: 73 690 4545
Fragments arranged by size:
4545 690 73
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 4084 618
Size: 325 217 627 842 1455 1842
Fragments arranged by size:
1842 1455 842 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 2627 1391
Size: 762 474 4072
Fragments arranged by size:
4072 762 474
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BbvCI CC'TCA_GC
Cuts at: 2595 2595
Size: 5308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3499 3533 1903
Size: 34 1562 34 3678
Fragments arranged by size:
3678 1562 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2097 2597 3919
Size: 107 122 71 631 605 425 500 1322
Cuts at: 3919 136
Size: 1525
Fragments arranged by size:
1525 1322 631 605 500 425 122 107
71
BglII A'GATC_T
Cuts at: 5303 5303
Size: 5308
BlpI GC'TnA_GC
Cuts at: 2042 2243 2725 2042
Size: 201 482 4625
Fragments arranged by size:
4625 482 201
Bme1580I G_kGCm'C
Cuts at: 1790 2093 3369 4525 1790
Size: 303 1276 1156 2573
Fragments arranged by size:
2573 1276 1156 303
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 3997 442
Size: 416 53 83 399 24 1622 958 1753
Fragments arranged by size:
1753 1622 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 3968 1239
Size: 1298 1431 2579
Fragments arranged by size:
2579 1431 1298
Bpu10I CC'TnA_GC
Cuts at: 2147 2595 2147
Size: 448 4860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2240 3433 4298 4596 2123
Size: 117 1193 865 298 2835
Fragments arranged by size:
2835 1193 865 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3971 882
Size: 617 2472 2219
Fragments arranged by size:
2472 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2170 2233 3099 493
Size: 711 966 63 866 2702
Fragments arranged by size:
2702 966 866 711 63
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2436 2481 3115 3497
Size: 53 83 186 936 903 45 634 382
Cuts at: 3497 4132 275
Size: 635 1451
Fragments arranged by size:
1451 936 903 635 634 382 186 83
53 45
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2262 2292 1178
Size: 30 165 30 609 30 220 30 4194
Fragments arranged by size:
4194 609 220 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2417 2588 1186
Size: 865 366 171 3906
Fragments arranged by size:
3906 865 366 171
BseYI C'CCAG_C
Cuts at: 4511 5007 4511
Size: 496 4812
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4735
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3519 4547 1540
Size: 275 186 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 3369 3454 4525 3369
Size: 85 1071 4152
Fragments arranged by size:
4152 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2153 2627 3369 3454 4525 1391
Size: 399 303 60 474 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 303 85 60
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 2079 3197 4927 2079
Size: 1118 1730 2460
Fragments arranged by size:
2460 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2603 3952 4139 1700
Size: 110 793 1349 187 2869
Fragments arranged by size:
2869 1349 793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2055 2319 794
Size: 1261 264 3783
Fragments arranged by size:
3783 1261 264
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303
Size: 666 367 385 17 1452 86 12 346
Cuts at: 5303 1972
Size: 1977
Fragments arranged by size:
1977 1452 666 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3817
Fragments arranged by size:
3817 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 487
Size: 683 56 1120 3449
Fragments arranged by size:
3449 1120 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DraIII CAC_nnn'GTG
Cuts at: 2055 2055
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2157 2755
Size: 54 1425 50 275 18 268 59 598
Cuts at: 2755 3644 5273 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 598 275 268 59 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 3238 1353
Size: 1107 778 3423
Fragments arranged by size:
3423 1107 778
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 3895 4269 4415 387
Size: 1064 138 522 1784 374 146 1280
Fragments arranged by size:
1784 1280 1064 522 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 3371 3968 4755 5023 1239
Size: 393 905 834 597 787 268 1524
Fragments arranged by size:
1524 905 834 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FspI TGC'GCA
Cuts at: 2546 3814 2546
Size: 1268 4040
HaeII r_GCGC'y
Cuts at: 1678 2243 4455 1678
Size: 565 2212 2531
Fragments arranged by size:
2531 2212 565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 3949 3981 4023
Size: 32 135 32 440 32 1905 32 42
Cuts at: 4023 4055 1373
Size: 32 2626
Fragments arranged by size:
2626 1905 440 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 2652 2898 669
Size: 988 281 502 212 246 3079
Fragments arranged by size:
3079 988 502 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 3218
Fragments arranged by size:
3218 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 3625 3784 518
Size: 648 2100 359 159 2042
Fragments arranged by size:
2100 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2283 2594 2724 3401 4549 4794 1606
Size: 677 311 130 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 677 311 245 130
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 5308
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3817
Fragments arranged by size:
3817 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2170 1108
Size: 1062 4246
PfoI T'CCnGG_A
Cuts at: 2502 2502
Size: 5308
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2294 3377 3409 4890 4922 2262
Size: 32 1083 32 1481 32 2648
Fragments arranged by size:
2648 1481 1083 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2060 2092 2060
Size: 32 5276
PstI C_TGCA'G
Cuts at: 830 2381 2401 2660 830
Size: 1551 20 259 3478
Fragments arranged by size:
3478 1551 259 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2377 2397 2656 3791 4472 4663 826
Size: 219 1332 20 259 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 681 259 219 191 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2255 3412 4313 4575 820
Size: 197 926 195 117 1157 901 262 1553
Fragments arranged by size:
1553 1157 926 901 262 197 195 117
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2747 513
Size: 552 939 743 3074
Fragments arranged by size:
3074 939 743 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 3352 3505 3690 4135 325
Size: 1255 1755 17 153 185 445 1498
Fragments arranged by size:
1755 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 5268 5300 2016
Size: 32 3220 32 2024
Fragments arranged by size:
3220 2024 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2437 3116 276
Size: 53 83 186 936 903 679 2468
Fragments arranged by size:
2468 936 903 679 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI