Restriction map for
pFN21AE6210
(Circular) (Six-base) MAPSORT of: pFN21AE6210.seq Check: 5299 from: 1 to: 5455
pFN21AE6210 5455 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2562 3265 278
Size: 53 83 186 936 1026 703 2468
Fragments arranged by size:
2468 1026 936 703 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 3518 4902 5170 1632
Size: 578 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1308 578 268
AfeI AGC'GCT
Cuts at: 2309 2309
Size: 5455
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2735 1166
Size: 1569 3886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 2159
Size: 32 5423
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2156 2764 2990 3142 5251 1493
Size: 663 608 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2367 2780 1870
Size: 73 194 230 413 4545
Fragments arranged by size:
4545 413 230 194 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2626 2708 2776 4231
Size: 325 217 627 345 494 82 68 1455
Cuts at: 4231 618
Size: 1842
Fragments arranged by size:
1842 1455 627 494 345 325 217 82
68
BanII G_rGCy'C
Cuts at: 1391 2063 2102 2622 2774 1391
Size: 672 39 520 152 4072
Fragments arranged by size:
4072 672 520 152 39
BbeI G_GCGC'C
Cuts at: 2136 2136
Size: 5455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BbvCI CC'TCA_GC
Cuts at: 2548 2548
Size: 5455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 2694 4066
Size: 107 122 71 631 605 412 610 1372
Cuts at: 4066 136
Size: 1525
Fragments arranged by size:
1525 1372 631 610 605 412 122 107
71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2547 2671 2711 2732 3516 4672 1790
Size: 757 124 40 21 784 1156 2573
Fragments arranged by size:
2573 1156 784 757 124 40 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 3186 4144
Size: 416 53 83 399 24 1176 593 958
Cuts at: 4144 442
Size: 1753
Fragments arranged by size:
1753 1176 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4115 1239
Size: 1367 1509 2579
Fragments arranged by size:
2579 1509 1367
Bpu10I CC'TnA_GC
Cuts at: 2057 2548 2057
Size: 491 4964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 4118 882
Size: 617 1149 1470 2219
Fragments arranged by size:
2219 1470 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 2435 5449 2435
Size: 3014 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2212 2559 3262
Size: 53 83 186 936 600 79 347 703
Cuts at: 3262 3644 4279 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 703 635 600 382 347 186
83 79 53
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4519
Fragments arranged by size:
4519 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 1186
Size: 1408 4047
BseYI C'CCAG_C
Cuts at: 2377 2499 4658 5154 2377
Size: 122 2159 496 2678
Fragments arranged by size:
2678 2159 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4882
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 2583 2622 3516 3601 4672 2358
Size: 225 39 894 85 1071 3141
Fragments arranged by size:
3141 1071 894 225 85 39
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2102 2358 2547 2583 2622 2671
Size: 399 273 39 256 189 36 39 49
Cuts at: 2671 2711 2732 2774 3516 3601 4672 1391
Size: 40 21 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 273 256 189 85
49 42 40 39 39 36 21
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 2810 844
Size: 1697 269 3489
Fragments arranged by size:
3489 1697 269
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3950 4124 65
Size: 1183 2702 174 1396
Fragments arranged by size:
2702 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2264 4099 4286 1700
Size: 110 454 1835 187 2869
Fragments arranged by size:
2869 1835 454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 2702 3515 4527 2702
Size: 813 1012 3630
Fragments arranged by size:
3630 1012 813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 2347 1184
Size: 1163 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2689
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450
Size: 813 367 385 17 1452 86 12 346
Cuts at: 5450 1972
Size: 1977
Fragments arranged by size:
1977 1452 813 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2606 2606
Size: 5455
BtgI C'CryG_G
Cuts at: 513 1065 2004 2342 2649 513
Size: 552 939 338 307 3319
Fragments arranged by size:
3319 939 552 338 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 3688
Fragments arranged by size:
3688 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2455 2679
Size: 54 1425 50 275 18 432 193 224
Cuts at: 2679 2902 3791 5420 8
Size: 223 889 1629 43
Fragments arranged by size:
1629 1425 889 432 275 224 223 193
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3385 1353
Size: 2032 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4042 4416 4562 387
Size: 1064 138 2453 374 146 1280
Fragments arranged by size:
2453 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2620 2620
Size: 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2606 3518 4115 4902 5170 1239
Size: 393 578 396 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 578 396 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2587 2587
Size: 5455
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 2450 3961 2450
Size: 1511 3944
HaeII r_GCGC'y
Cuts at: 1678 2136 2311 4602 1678
Size: 458 175 2291 2531
Fragments arranged by size:
2531 2291 458 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373
Size: 32 135 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KasI G'GCGC_C
Cuts at: 2132 2132
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 4569 4753 1305
Size: 1032 2232 184 2007
Fragments arranged by size:
2232 2007 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2681 10
Size: 54 1425 343 849 2784
Fragments arranged by size:
2784 1425 849 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2300 2333 2723 2735 3413 3772
Size: 648 879 255 33 390 12 678 359
Cuts at: 3772 3931 518
Size: 159 2042
Fragments arranged by size:
2042 879 678 648 390 359 255 159
33 12
MspA1I CmG'CkG
Cuts at: 1606 2173 2871 3548 4696 4941 1606
Size: 567 698 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 567 245
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 5455
NarI GG'CG_CC
Cuts at: 2133 2133
Size: 5455
NcoI C'CATG_G
Cuts at: 513 1065 2004 2649 513
Size: 552 939 645 3319
Fragments arranged by size:
3319 939 645 552
NdeI CA'TA_TG
Cuts at: 387 2734 387
Size: 2347 3108
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2813 4358 1132
Size: 97 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2671 2751 1348
Size: 1323 80 4052
Fragments arranged by size:
4052 1323 80
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2660 1613
Size: 1047 4408
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2807 830
Size: 1977 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
PvuII CAG'CTG
Cuts at: 2173 2173
Size: 5455
SacI G_AGCT'C
Cuts at: 2622 2622
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2803 3938 4619 4810 826
Size: 219 1758 1135 681 191 1471
Fragments arranged by size:
1758 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2134 2134
Size: 5455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2782 1872
Size: 910 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3559 4460 4722 820
Size: 197 926 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2649 2894 513
Size: 552 939 78 567 245 3074
Fragments arranged by size:
3074 939 567 552 245 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 3482 3499 3652 3837 4282 325
Size: 1255 585 1317 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 5415 5447 2084
Size: 32 3299 32 2092
Fragments arranged by size:
3299 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 2656 2689 2368
Size: 288 33 5134
Fragments arranged by size:
5134 288 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2780 1870
Size: 910 4545
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2560 3263 276
Size: 53 83 186 936 1026 703 2468
Fragments arranged by size:
2468 1026 936 703 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI