Restriction map for
pFN21AE6209
(Circular) (Six-base) MAPSORT of: pFN21AE6209.seq Check: 8146 from: 1 to: 5464
pFN21AE6209 5464 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2125 2125
Size: 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2706 2807 1937
Size: 769 101 4594
Fragments arranged by size:
4594 769 101
Acc65I G'GTAC_C
Cuts at: 2642 2785 2642
Size: 143 5321
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AfeI AGC'GCT
Cuts at: 2192 2192
Size: 5464
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 2714 1166
Size: 1548 3916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 3207 4779 3207
Size: 1572 3892
ApaLI G'TGCA_C
Cuts at: 2237 3521 4677 2237
Size: 1284 1156 3024
Fragments arranged by size:
3024 1284 1156
ApoI r'AATT_y
Cuts at: 1493 2560 2773 2999 3151 5260 1493
Size: 1067 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 1870
Size: 73 846 4545
Fragments arranged by size:
4545 846 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2642 2785 4240 618
Size: 325 217 627 315 540 143 1455 1842
Fragments arranged by size:
1842 1455 627 540 325 315 217 143
BanII G_rGCy'C
Cuts at: 1391 2275 2468 2783 1391
Size: 884 193 315 4072
Fragments arranged by size:
4072 884 315 193
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 5464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2310 4075 136
Size: 107 122 71 631 605 638 1765 1525
Fragments arranged by size:
1765 1525 638 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2456 3525 4681 1790
Size: 451 215 1069 1156 2573
Fragments arranged by size:
2573 1156 1069 451 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 4153 442
Size: 416 53 83 399 24 1778 958 1753
Fragments arranged by size:
1778 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4124 1239
Size: 2885 2579
Bpu10I CC'TnA_GC
Cuts at: 2050 2050
Size: 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2697 3589 4454 4752 2697
Size: 892 865 298 3409
Fragments arranged by size:
3409 892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2175 3271 3653
Size: 53 83 186 936 570 72 1096 382
Cuts at: 3653 4288 275
Size: 635 1451
Fragments arranged by size:
1451 1096 936 635 570 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5464
BseYI C'CCAG_C
Cuts at: 2193 2257 2448 4667 5163 2193
Size: 64 191 2219 496 2494
Fragments arranged by size:
2494 2219 496 191 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2515 2656 1163
Size: 573 779 141 3971
Fragments arranged by size:
3971 779 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2241 2275 3525 3610 4681 2202
Size: 39 34 1250 85 1071 2985
Fragments arranged by size:
2985 1250 1071 85 39 34
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2241 2275 2456 2468 2783 3525
Size: 399 412 39 34 181 12 315 742
Cuts at: 3525 3610 4681 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 315 181 85
39 34 12
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 2819 844
Size: 1281 694 3489
Fragments arranged by size:
3489 1281 694
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 3959 4133 65
Size: 1183 1366 1345 174 1396
Fragments arranged by size:
1396 1366 1345 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 2348 2461 3524 4536 2348
Size: 113 1063 1012 3276
Fragments arranged by size:
3276 1063 1012 113
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2012 2034 2794 3161 3546 3563 5015 5101
Size: 40 22 760 367 385 17 1452 86
Cuts at: 5101 5113 5459 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 86 40
22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2129 2382 513
Size: 552 939 125 253 3595
Fragments arranged by size:
3595 939 552 253 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2744 487
Size: 683 56 1518 3207
Fragments arranged by size:
3207 1518 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 2202 2212 2254 2980 3774 3794 2164
Size: 38 10 42 726 794 20 3834
Fragments arranged by size:
3834 794 726 42 38 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DraIII CAC_nnn'GTG
Cuts at: 2733 2733
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2702 4471 809
Size: 507 1386 1769 1802
Fragments arranged by size:
1802 1769 1386 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2683 2911 3800
Size: 54 1425 50 275 18 853 228 889
Cuts at: 3800 5429 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 853 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2291 4051 4425 4571 387
Size: 1064 138 702 1760 374 146 1280
Fragments arranged by size:
1760 1280 1064 702 374 146 138
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3527 4124 4911 5179 1239
Size: 393 1895 597 787 268 1524
Fragments arranged by size:
1895 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2106 2194 4611 1678
Size: 428 88 2417 2531
Fragments arranged by size:
2531 2417 428 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2004 2036 4105 4137 4179
Size: 32 135 32 432 32 2069 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 2069 432 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 5464
KpnI G_GTAC'C
Cuts at: 2646 2789 2646
Size: 143 5321
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3642
Fragments arranged by size:
3642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 2348 2498 2714 3422 3781 3940
Size: 648 1168 14 150 216 708 359 159
Cuts at: 3940 518
Size: 2042
Fragments arranged by size:
2042 1168 708 648 359 216 159 150
14
MspA1I CmG'CkG
Cuts at: 1606 2412 2880 3557 4705 4950 1606
Size: 806 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 806 677 468 245
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 2004 2129 2382 513
Size: 552 939 125 253 3595
Fragments arranged by size:
3595 939 552 253 125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2080 1348
Size: 732 4732
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2419 2451 2419
Size: 32 5432
PstI C_TGCA'G
Cuts at: 830 2034 2068 2816 830
Size: 1204 34 748 3478
Fragments arranged by size:
3478 1204 748 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2030 2064 2812 3947 4628 4819 826
Size: 219 985 34 748 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 748 681 219 191 34
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 5464
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2712 3568 4469 4731 820
Size: 197 926 769 856 901 262 1553
Fragments arranged by size:
1553 926 901 856 769 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 1517
Size: 771 4693
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2129 2382 2903 513
Size: 552 939 51 74 253 521 3074
Fragments arranged by size:
3074 939 552 521 253 74 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 2185 3491 3508 3661 3846 4291
Size: 1255 562 43 1306 17 153 185 445
Cuts at: 4291 325
Size: 1498
Fragments arranged by size:
1498 1306 1255 562 445 185 153 43
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 2435 3710
Size: 274 80 33 51 1443 252 206 1275
Cuts at: 3710 5422 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1275 274 252 206 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 5424 5456 2616
Size: 32 2776 32 2624
Fragments arranged by size:
2776 2624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 2297 3593 2297
Size: 1296 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI