Restriction map for
pFN21AE6207
(Circular) (Six-base) MAPSORT of: pFN21AE6207.seq Check: 6586 from: 1 to: 5533
pFN21AE6207 5533 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2313 2313
Size: 5533
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2778 2876 1937
Size: 841 98 4594
Fragments arranged by size:
4594 841 98
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 2832 3660 4033 1563
Size: 1269 828 373 3063
Fragments arranged by size:
3063 1269 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 3596 4980 5248 1632
Size: 668 1296 1384 268 1917
Fragments arranged by size:
1917 1384 1296 668 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 2799 4432 5409 1203
Size: 1596 1633 977 1327
Fragments arranged by size:
1633 1596 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaI G_GGCC'C
Cuts at: 2457 2470 2457
Size: 13 5520
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2286 2858 1870
Size: 73 103 240 572 4545
Fragments arranged by size:
4545 572 240 103 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2150 2528 2854 4309
Size: 325 217 627 255 108 378 326 1455
Cuts at: 4309 618
Size: 1842
Fragments arranged by size:
1842 1455 627 378 326 325 255 217
108
BanII G_rGCy'C
Cuts at: 1391 2014 2053 2287 2378 2457 2470 2597 2852
Size: 623 39 234 91 79 13 127 255
Cuts at: 2852 1391
Size: 4072
Fragments arranged by size:
4072 623 255 234 127 91 79 39
13
BbeI G_GCGC'C
Cuts at: 2046 2154 2046
Size: 108 5425
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 928
Size: 971 706 3856
Fragments arranged by size:
3856 971 706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 3724 3758 1903
Size: 34 110 34 1643 34 3678
Fragments arranged by size:
3678 1643 110 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 4144 136
Size: 107 122 71 631 605 427 2045 1525
Fragments arranged by size:
2045 1525 631 605 427 122 107 71
BglII A'GATC_T
Cuts at: 2787 5528 2787
Size: 2741 2792
BlpI GC'TnA_GC
Cuts at: 2493 2950 2493
Size: 457 5076
Bme1580I G_kGCm'C
Cuts at: 1790 2457 2470 2531 3594 4750 1790
Size: 667 13 61 1063 1156 2573
Fragments arranged by size:
2573 1156 1063 667 61 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2560 2712 4193 1239
Size: 1321 152 1481 2579
Fragments arranged by size:
2579 1481 1321 152
Bpu10I CC'TnA_GC
Cuts at: 2377 2591 2377
Size: 214 5319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2191 2496 2708 4196 882
Size: 617 692 305 212 1488 2219
Fragments arranged by size:
2219 1488 692 617 305 212
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2151 3340 3722
Size: 53 83 186 936 510 108 1189 382
Cuts at: 3722 4357 275
Size: 635 1451
Fragments arranged by size:
1451 1189 936 635 510 382 186 108
83 53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5533
BseYI C'CCAG_C
Cuts at: 2523 2763 4736 5232 2523
Size: 240 1973 496 2824
Fragments arranged by size:
2824 1973 496 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2256 2538 2983 3744 4772 1540
Size: 275 186 255 282 445 761 1028 2301
Fragments arranged by size:
2301 1028 761 445 282 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 2014 2412 2494 3594 3679 4750 2014
Size: 398 82 1100 85 1071 2797
Fragments arranged by size:
2797 1100 1071 398 85 82
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2053 2287 2378 2412 2457 2470
Size: 399 224 39 234 91 34 45 13
Cuts at: 2470 2494 2531 2597 2852 3594 3679 4750 1391
Size: 24 37 66 255 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 255 234 224 91
85 66 45 39 37 34 24 13
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2173 2313 2579 2888 844
Size: 1329 140 266 309 3489
Fragments arranged by size:
3489 1329 309 266 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 2160 2162 1925
Size: 235 2 5296
Fragments arranged by size:
5296 235 2
BssSI C'ACGA_G
Cuts at: 2734 3593 4605 2734
Size: 859 1012 3662
Fragments arranged by size:
3662 1012 859
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 2519 2519
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2787 2863 3230 3615 3632 5084 5170 5182
Size: 815 76 367 385 17 1452 86 12
Cuts at: 5182 5528 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 815 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 3514
Fragments arranged by size:
3514 1467 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 2212 2260 3049 3843 3863 2212
Size: 48 789 794 20 3882
Fragments arranged by size:
3882 794 789 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2433 2535
Size: 54 1425 50 275 18 423 180 102
Cuts at: 2535 2980 3869 5498 8
Size: 445 889 1629 43
Fragments arranged by size:
1629 1425 889 445 423 275 180 102
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2253 2535 2980 1537
Size: 275 441 282 445 4090
Fragments arranged by size:
4090 445 441 282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 3463 1353
Size: 845 1265 3423
Fragments arranged by size:
3423 1265 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2020 2321 4120 4494 4640 387
Size: 1064 138 431 301 1799 374 146 1280
Fragments arranged by size:
1799 1280 1064 431 374 301 146 138
EcoICRI GAG'CTC
Cuts at: 2012 2012
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2560 2712 3596 4193 4980 5248
Size: 393 668 260 152 884 597 787 268
Cuts at: 5248 1239
Size: 1524
Fragments arranged by size:
1524 884 787 668 597 393 268 260
152
EcoO109I rG'GnC_Cy
Cuts at: 2381 2453 2454 2467 2563 2671 2696 2381
Size: 72 1 13 96 108 25 5218
Fragments arranged by size:
5218 108 96 72 25 13 1
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2046 2108 2154 4680 1678
Size: 368 62 46 2526 2531
Fragments arranged by size:
2531 2526 368 62 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4174 4206 4248 4280 1373
Size: 32 135 32 2602 32 42 32 2626
Fragments arranged by size:
2626 2602 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KasI G'GCGC_C
Cuts at: 2042 2150 2042
Size: 108 5425
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 4647 4831 1305
Size: 1548 1794 184 2007
Fragments arranged by size:
2007 1794 1548 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 3108
Fragments arranged by size:
3108 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3850 4009 518
Size: 648 2325 359 159 2042
Fragments arranged by size:
2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2487 2774 2949 3626 4774 5019 1606
Size: 465 416 287 175 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 465 416 287 245 175
NarI GG'CG_CC
Cuts at: 2043 2151 2043
Size: 108 5425
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4981
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2803 2891 4436 1132
Size: 97 1574 88 1545 2229
Fragments arranged by size:
2229 1574 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 2799 4432 2799
Size: 1633 3900
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2804 2804
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2671
Size: 5533
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PspOMI G'GGCC_C
Cuts at: 2453 2466 2453
Size: 13 5520
PspXI vC'TCGA_Gb
Cuts at: 2286 2286
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2475 2885 830
Size: 1645 410 3478
Fragments arranged by size:
3478 1645 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2774 2774
Size: 5533
SacI G_AGCT'C
Cuts at: 2014 2014
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 5533
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2471 2881 4016 4697 4888 826
Size: 219 1426 410 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 681 410 219 191
SfoI GGC'GCC
Cuts at: 2044 2152 2044
Size: 108 5425
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2048 2860 1872
Size: 176 812 4545
Fragments arranged by size:
4545 812 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2286 3637 4538 4800 820
Size: 197 926 343 1351 901 262 1553
Fragments arranged by size:
1553 1351 926 901 343 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SrfI GCCC'GGGC
Cuts at: 2048 2048
Size: 5533
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2393 2722 2972 513
Size: 552 1328 329 250 3074
Fragments arranged by size:
3074 1328 552 329 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 2798 3560 3577 3730 3915 4360
Size: 1255 653 565 762 17 153 185 445
Cuts at: 4360 325
Size: 1498
Fragments arranged by size:
1498 1255 762 653 565 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2553 3779 5491
Size: 274 80 33 51 1443 576 1226 1712
Cuts at: 5491 96
Size: 138
Fragments arranged by size:
1712 1443 1226 576 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2687 2687
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 2286 1943
Size: 343 5190
XmaI C'CCGG_G
Cuts at: 1870 2046 2858 1870
Size: 176 812 4545
Fragments arranged by size:
4545 812 176
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PsrI RsrII SacII SanDI SexAI SfiI SwaI