(Circular) (Six-base) MAPSORT of: pFN21AE6203.seq Check: 6424 from: 1 to: 5374 pFN21AE6203 5374 bp With 182 enzymes: SgfI * May 20, 2014 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 3437 4821 5089 1632 Size: 928 877 1384 268 1917 Fragments arranged by size: 1917 1384 928 877 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 3117 4689 3117 Size: 1572 3802 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2353 2625 2699 1870 Size: 73 410 272 74 4545 Fragments arranged by size: 4545 410 272 74 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 2327 2396 2420 2695 Size: 325 217 627 489 51 69 24 275 Cuts at: 2695 4150 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 489 325 275 217 69 51 24 BanII G_rGCy'C Cuts at: 1391 2342 2646 2693 1391 Size: 951 304 47 4072 Fragments arranged by size: 4072 951 304 47 BbeI G_GCGC'C Cuts at: 2280 2280 Size: 5374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2104 2431 2566 928 Size: 971 205 327 135 3736 Fragments arranged by size: 3736 971 327 205 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3565 3599 1903 Size: 34 50 34 1544 34 3678 Fragments arranged by size: 3678 1544 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2374 3266 4476 2374 Size: 892 1210 3272 Fragments arranged by size: 3272 1210 892 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 136 Size: 107 122 71 631 605 2313 1525 Fragments arranged by size: 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2373 5369 2373 Size: 2996 2378 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2423 3435 4591 1790 Size: 633 1012 1156 2573 Fragments arranged by size: 2573 1156 1012 633 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 3105 4063 Size: 416 53 83 399 24 957 731 958 Cuts at: 4063 442 Size: 1753 Fragments arranged by size: 1753 958 957 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4034 1239 Size: 2795 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2660 3499 4364 4662 2660 Size: 839 865 298 3372 Fragments arranged by size: 3372 865 839 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2221 3165 493 Size: 711 1017 944 2702 Fragments arranged by size: 2702 1017 944 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2277 2532 3181 3563 Size: 53 83 186 936 744 255 649 382 Cuts at: 3563 4198 275 Size: 635 1451 Fragments arranged by size: 1451 936 744 649 635 382 255 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 1178 Size: 30 165 30 825 30 4294 Fragments arranged by size: 4294 825 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 1186 Size: 1063 4311 BseYI C'CCAG_C Cuts at: 2092 2153 4577 5073 2092 Size: 61 2424 496 2393 Fragments arranged by size: 2424 2393 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 2276 1163 Size: 573 456 84 4261 Fragments arranged by size: 4261 573 456 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2527 2824 3585 4613 1540 Size: 275 186 526 297 761 1028 2301 Fragments arranged by size: 2301 1028 761 526 297 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 3435 3520 4591 2646 Size: 789 85 1071 3429 Fragments arranged by size: 3429 1071 789 85 BsmI GAATG_Cn' Cuts at: 2263 2885 2978 2263 Size: 622 93 4659 Fragments arranged by size: 4659 622 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2447 2447 Size: 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2342 2423 2646 2693 3435 3520 4591 Size: 399 552 81 223 47 742 85 1071 Cuts at: 4591 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 552 399 223 85 81 47 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2022 4018 4205 1700 Size: 110 212 1996 187 2869 Fragments arranged by size: 2869 1996 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2165 3434 4446 2165 Size: 1269 1012 3093 Fragments arranged by size: 3093 1269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstXI CCAn_nnnn'nTGG Cuts at: 2508 2508 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2373 2704 3071 3456 3473 4925 5011 5023 Size: 401 331 367 385 17 1452 86 12 Cuts at: 5023 5369 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 401 385 367 346 331 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2344 513 Size: 552 951 328 3543 Fragments arranged by size: 3543 951 552 328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2249 2890 3684 3704 2015 Size: 234 641 794 20 3685 Fragments arranged by size: 3685 794 641 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2469 2504 529 Size: 35 1905 35 3399 Fragments arranged by size: 3399 1905 35 35 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 2524 2562 Size: 54 1425 50 275 18 517 177 38 Cuts at: 2562 2662 2821 3710 5339 8 Size: 100 159 889 1629 43 Fragments arranged by size: 1629 1425 889 517 275 177 159 100 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2524 2821 1537 Size: 275 712 297 4090 Fragments arranged by size: 4090 712 297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2327 2606 2616 3304 1353 Size: 765 209 279 10 688 3423 Fragments arranged by size: 3423 765 688 279 209 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2644 2644 Size: 5374 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2560 3437 4034 4821 5089 1239 Size: 393 928 877 597 787 268 1524 Fragments arranged by size: 1524 928 877 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2629 2668 2629 Size: 39 5335 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2280 2362 4521 1678 Size: 602 82 2159 2531 Fragments arranged by size: 2531 2159 602 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2276 2276 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2664 10 Size: 54 1425 343 832 2720 Fragments arranged by size: 2720 1425 832 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2510 3332 3691 3850 518 Size: 648 1344 822 359 159 2042 Fragments arranged by size: 2042 1344 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2346 2486 2790 3467 4615 4860 1606 Size: 457 283 140 304 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 457 304 283 245 140 NaeI GCC'GGC Cuts at: 2024 2024 Size: 5374 NarI GG'CG_CC Cuts at: 2277 2277 Size: 5374 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NgoMIV G'CCGG_C Cuts at: 2022 2022 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 2447 1348 Size: 1099 4275 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2170 1108 Size: 902 160 4312 Fragments arranged by size: 4312 902 160 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PmlI CAC'GTG Cuts at: 2221 2221 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2668 2668 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 2075 1613 Size: 462 4912 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2244 2726 830 Size: 1414 482 3478 Fragments arranged by size: 3478 1414 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 PvuII CAG'CTG Cuts at: 2486 2486 Size: 5374 SacI G_AGCT'C Cuts at: 2646 2646 Size: 5374 SacII CC_GC'GG Cuts at: 2347 2347 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SapI GCTCTTCn'nnn_ Cuts at: 2327 2327 Size: 5374 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2240 2722 3857 4538 4729 826 Size: 219 1195 482 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 482 219 191 SfoI GGC'GCC Cuts at: 2278 2278 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3478 4379 4641 820 Size: 197 926 696 839 901 262 1553 Fragments arranged by size: 1553 926 901 839 696 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2334 2813 513 Size: 552 1269 479 3074 Fragments arranged by size: 3074 1269 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2226 2236 2597 3401 3418 3571 3756 Size: 1255 646 10 361 804 17 153 185 Cuts at: 3756 4201 325 Size: 445 1498 Fragments arranged by size: 1498 1255 804 646 445 361 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3620 5332 Size: 274 80 33 51 1443 484 1159 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1443 1159 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 5334 5366 2228 Size: 32 3074 32 2236 Fragments arranged by size: 3074 2236 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2570 1316 Size: 1254 4120 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI