Restriction map for
pFN21AE6203
(Circular) (Six-base) MAPSORT of: pFN21AE6203.seq Check: 6424 from: 1 to: 5374
pFN21AE6203 5374 bp
With 182 enzymes: SgfI *
May 20, 2014 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 3437 4821 5089 1632
Size: 928 877 1384 268 1917
Fragments arranged by size:
1917 1384 928 877 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 3117 4689 3117
Size: 1572 3802
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2353 2625 2699 1870
Size: 73 410 272 74 4545
Fragments arranged by size:
4545 410 272 74 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2327 2396 2420 2695
Size: 325 217 627 489 51 69 24 275
Cuts at: 2695 4150 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 489 325 275 217 69
51 24
BanII G_rGCy'C
Cuts at: 1391 2342 2646 2693 1391
Size: 951 304 47 4072
Fragments arranged by size:
4072 951 304 47
BbeI G_GCGC'C
Cuts at: 2280 2280
Size: 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 2431 2566 928
Size: 971 205 327 135 3736
Fragments arranged by size:
3736 971 327 205 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3565 3599 1903
Size: 34 50 34 1544 34 3678
Fragments arranged by size:
3678 1544 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2374 3266 4476 2374
Size: 892 1210 3272
Fragments arranged by size:
3272 1210 892
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 136
Size: 107 122 71 631 605 2313 1525
Fragments arranged by size:
2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2373 5369 2373
Size: 2996 2378
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2423 3435 4591 1790
Size: 633 1012 1156 2573
Fragments arranged by size:
2573 1156 1012 633
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 3105 4063
Size: 416 53 83 399 24 957 731 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 958 957 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4034 1239
Size: 2795 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 3499 4364 4662 2660
Size: 839 865 298 3372
Fragments arranged by size:
3372 865 839 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2221 3165 493
Size: 711 1017 944 2702
Fragments arranged by size:
2702 1017 944 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 2532 3181 3563
Size: 53 83 186 936 744 255 649 382
Cuts at: 3563 4198 275
Size: 635 1451
Fragments arranged by size:
1451 936 744 649 635 382 255 186
83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4294
Fragments arranged by size:
4294 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 4311
BseYI C'CCAG_C
Cuts at: 2092 2153 4577 5073 2092
Size: 61 2424 496 2393
Fragments arranged by size:
2424 2393 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2276 1163
Size: 573 456 84 4261
Fragments arranged by size:
4261 573 456 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2527 2824 3585 4613 1540
Size: 275 186 526 297 761 1028 2301
Fragments arranged by size:
2301 1028 761 526 297 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 3435 3520 4591 2646
Size: 789 85 1071 3429
Fragments arranged by size:
3429 1071 789 85
BsmI GAATG_Cn'
Cuts at: 2263 2885 2978 2263
Size: 622 93 4659
Fragments arranged by size:
4659 622 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2447 2447
Size: 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2342 2423 2646 2693 3435 3520 4591
Size: 399 552 81 223 47 742 85 1071
Cuts at: 4591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 552 399 223 85 81
47
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 4018 4205 1700
Size: 110 212 1996 187 2869
Fragments arranged by size:
2869 1996 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 2165 3434 4446 2165
Size: 1269 1012 3093
Fragments arranged by size:
3093 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstXI CCAn_nnnn'nTGG
Cuts at: 2508 2508
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2373 2704 3071 3456 3473 4925 5011 5023
Size: 401 331 367 385 17 1452 86 12
Cuts at: 5023 5369 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 401 385 367 346 331 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2344 513
Size: 552 951 328 3543
Fragments arranged by size:
3543 951 552 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2249 2890 3684 3704 2015
Size: 234 641 794 20 3685
Fragments arranged by size:
3685 794 641 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2469 2504 529
Size: 35 1905 35 3399
Fragments arranged by size:
3399 1905 35 35
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 2524 2562
Size: 54 1425 50 275 18 517 177 38
Cuts at: 2562 2662 2821 3710 5339 8
Size: 100 159 889 1629 43
Fragments arranged by size:
1629 1425 889 517 275 177 159 100
54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2524 2821 1537
Size: 275 712 297 4090
Fragments arranged by size:
4090 712 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2327 2606 2616 3304 1353
Size: 765 209 279 10 688 3423
Fragments arranged by size:
3423 765 688 279 209 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2644 2644
Size: 5374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2560 3437 4034 4821 5089 1239
Size: 393 928 877 597 787 268 1524
Fragments arranged by size:
1524 928 877 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2629 2668 2629
Size: 39 5335
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2280 2362 4521 1678
Size: 602 82 2159 2531
Fragments arranged by size:
2531 2159 602 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373
Size: 32 135 32 2443 32 42 32 2626
Fragments arranged by size:
2626 2443 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2276 2276
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2664 10
Size: 54 1425 343 832 2720
Fragments arranged by size:
2720 1425 832 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 3332 3691 3850 518
Size: 648 1344 822 359 159 2042
Fragments arranged by size:
2042 1344 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2346 2486 2790 3467 4615 4860 1606
Size: 457 283 140 304 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 457 304 283 245 140
NaeI GCC'GGC
Cuts at: 2024 2024
Size: 5374
NarI GG'CG_CC
Cuts at: 2277 2277
Size: 5374
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NgoMIV G'CCGG_C
Cuts at: 2022 2022
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 2447 1348
Size: 1099 4275
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2170 1108
Size: 902 160 4312
Fragments arranged by size:
4312 902 160
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PmlI CAC'GTG
Cuts at: 2221 2221
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2668 2668
Size: 5374
PshAI GACnn'nnGTC
Cuts at: 1613 2075 1613
Size: 462 4912
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2244 2726 830
Size: 1414 482 3478
Fragments arranged by size:
3478 1414 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
PvuII CAG'CTG
Cuts at: 2486 2486
Size: 5374
SacI G_AGCT'C
Cuts at: 2646 2646
Size: 5374
SacII CC_GC'GG
Cuts at: 2347 2347
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SapI GCTCTTCn'nnn_
Cuts at: 2327 2327
Size: 5374
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2240 2722 3857 4538 4729 826
Size: 219 1195 482 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 482 219 191
SfoI GGC'GCC
Cuts at: 2278 2278
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3478 4379 4641 820
Size: 197 926 696 839 901 262 1553
Fragments arranged by size:
1553 926 901 839 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2334 2813 513
Size: 552 1269 479 3074
Fragments arranged by size:
3074 1269 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 2236 2597 3401 3418 3571 3756
Size: 1255 646 10 361 804 17 153 185
Cuts at: 3756 4201 325
Size: 445 1498
Fragments arranged by size:
1498 1255 804 646 445 361 185 153
17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3620 5332
Size: 274 80 33 51 1443 484 1159 1712
Cuts at: 5332 96
Size: 138
Fragments arranged by size:
1712 1443 1159 484 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 5334 5366 2228
Size: 32 3074 32 2236
Fragments arranged by size:
3074 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2570 1316
Size: 1254 4120
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI XcmI