Restriction map for
pFN21AE6140
(Circular) (Six-base) MAPSORT of: pFN21AE6140.seq Check: 6316 from: 1 to: 5215
pFN21AE6140 5215 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2411 3025 278
Size: 53 83 186 936 875 614 2468
Fragments arranged by size:
2468 936 875 614 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2558 1937
Size: 621 4594
Acc65I G'GTAC_C
Cuts at: 2536 2536
Size: 5215
AclI AA'CG_TT
Cuts at: 1563 3342 3715 1563
Size: 1779 373 3063
Fragments arranged by size:
3063 1779 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 3278 4662 4930 1632
Size: 672 974 1384 268 1917
Fragments arranged by size:
1917 1384 974 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5018
AflIII A'CryG_T
Cuts at: 1203 2479 4114 5091 1203
Size: 1276 1635 977 1327
Fragments arranged by size:
1635 1327 1276 977
AgeI A'CCGG_T
Cuts at: 4046 4046
Size: 5215
AhdI GACnn_n'nnGTC
Cuts at: 2407 3944 2407
Size: 1537 3678
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 1184
Size: 1002 4213
AlwNI CAG_nnn'CTG
Cuts at: 2958 4530 2958
Size: 1572 3643
ApaLI G'TGCA_C
Cuts at: 3272 4428 3272
Size: 1156 4059
ApoI r'AATT_y
Cuts at: 1493 2524 2750 2902 5011 1493
Size: 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3769 160
Size: 872 2737 1606
Fragments arranged by size:
2737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
AvaI C'yCGr_G
Cuts at: 1870 1943 2540 1870
Size: 73 597 4545
Fragments arranged by size:
4545 597 73
AvrII C'CTAG_G
Cuts at: 2236 2236
Size: 5215
BamHI G'GATC_C
Cuts at: 2545 2912 2545
Size: 367 4848
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2536 3991 618
Size: 325 217 627 564 185 1455 1842
Fragments arranged by size:
1842 1455 627 564 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2190 2534 1391
Size: 799 344 4072
Fragments arranged by size:
4072 799 344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 3851
Fragments arranged by size:
3851 971 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3406 3440 1903
Size: 34 1469 34 3678
Fragments arranged by size:
3678 1469 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 4317 3107
Size: 1210 4005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 3826 136
Size: 107 122 71 631 605 436 1718 1525
Fragments arranged by size:
1718 1525 631 605 436 122 107 71
BglII A'GATC_T
Cuts at: 5210 5210
Size: 5215
BlpI GC'TnA_GC
Cuts at: 2632 2632
Size: 5215
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2458 3276 4432 1790
Size: 314 354 818 1156 2573
Fragments arranged by size:
2573 1156 818 354 314
BmgBI CAC'GTC
Cuts at: 1318 2266 2398 2482 1318
Size: 948 132 84 4051
Fragments arranged by size:
4051 948 132 84
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2946 3904 442
Size: 416 53 83 399 24 1529 958 1753
Fragments arranged by size:
1753 1529 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3875 1239
Size: 2636 2579
Bpu10I CC'TnA_GC
Cuts at: 2138 2138
Size: 5215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3340 4205 4503 3340
Size: 865 298 4052
Fragments arranged by size:
4052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3878 882
Size: 617 2379 2219
Fragments arranged by size:
2379 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3006 493
Size: 711 1802 2702
Fragments arranged by size:
2702 1802 711
BsaBI GATnn'nnATC
Cuts at: 2510 5209 2510
Size: 2699 2516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2244 2408 2461 3022
Size: 53 83 186 936 711 164 53 561
Cuts at: 3022 3404 4039 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 711 635 561 382 186 164
83 53 53
BsaWI w'CCGG_w
Cuts at: 2496 3648 4046 4320 4467 4878 2496
Size: 1152 398 274 147 411 2833
Fragments arranged by size:
2833 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4990
Fragments arranged by size:
4990 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2062 2074 2077 1186
Size: 873 3 12 3 4324
Fragments arranged by size:
4324 873 12 3 3
BseYI C'CCAG_C
Cuts at: 2200 4418 4914 2200
Size: 2218 496 2501
Fragments arranged by size:
2501 2218 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4642
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3426 4454 1540
Size: 275 186 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 3276 3361 4432 3276
Size: 85 1071 4059
Fragments arranged by size:
4059 1071 85
BsmI GAATG_Cn'
Cuts at: 2726 2819 2726
Size: 93 5122
BsmBI CGTCTCn'nnnn_
Cuts at: 2235 2235
Size: 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2190 2458 2534 3276 3361 4432
Size: 399 314 86 268 76 742 85 1071
Cuts at: 4432 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 314 268 86 85
76
BspEI T'CCGG_A
Cuts at: 2496 4878 2496
Size: 2382 2833
BspHI T'CATG_A
Cuts at: 2358 3104 4834 2358
Size: 746 1730 2739
Fragments arranged by size:
2739 1730 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2570 844
Size: 1726 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 3102 1394
Size: 429 213 1066 3507
Fragments arranged by size:
3507 1066 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 3710 3884 65
Size: 1183 2462 174 1396
Fragments arranged by size:
2462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3859 4046 1700
Size: 110 2049 187 2869
Fragments arranged by size:
2869 2049 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5215
BssSI C'ACGA_G
Cuts at: 3275 4287 3275
Size: 1012 4203
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5215
BstBI TT'CG_AA
Cuts at: 4019 4019
Size: 5215
BstEII G'GTnAC_C
Cuts at: 1184 2413 1184
Size: 1229 3986
BstYI r'GATC_y
Cuts at: 1972 2545 2912 3297 3314 4766 4852 4864 5210
Size: 573 367 385 17 1452 86 12 346
Cuts at: 5210 1972
Size: 1977
Fragments arranged by size:
1977 1452 573 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2124 513
Size: 552 1059 3604
Fragments arranged by size:
3604 1059 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4476
Fragments arranged by size:
4476 683 56
BtsI GCAGTG_nn'
Cuts at: 2731 3525 3545 2731
Size: 794 20 4401
Fragments arranged by size:
4401 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5180
DraI TTT'AAA
Cuts at: 2519 2519
Size: 5215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2294 2354 4222 809
Size: 507 978 60 1868 1802
Fragments arranged by size:
1868 1802 978 507 60
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2662 3551 5180
Size: 54 1425 50 275 18 832 889 1629
Cuts at: 5180 8
Size: 43
Fragments arranged by size:
1629 1425 889 832 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 1537
Size: 275 850 4090
Fragments arranged by size:
4090 850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2026 2062 2080 3145 1353
Size: 673 36 18 1065 3423
Fragments arranged by size:
3423 1065 673 36 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3802 4176 4322 387
Size: 1064 138 2213 374 146 1280
Fragments arranged by size:
2213 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 3278 3875 4662 4930 1239
Size: 393 672 974 597 787 268 1524
Fragments arranged by size:
1524 974 787 672 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2116 2134 2116
Size: 18 5197
EcoRI G'AATT_C
Cuts at: 2524 2902 2524
Size: 378 4837
FspI TGC'GCA
Cuts at: 3721 3721
Size: 5215
HaeII r_GCGC'y
Cuts at: 1678 4362 1678
Size: 2684 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2407 2425 2439 3856
Size: 32 135 32 821 14 18 14 1417
Cuts at: 3856 3888 3930 3962 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 821 135 42 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 2805 669
Size: 988 281 621 246 3079
Fragments arranged by size:
3079 988 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5215
HpaI GTT'AAC
Cuts at: 2805 2805
Size: 5215
KpnI G_GTAC'C
Cuts at: 2540 2540
Size: 5215
MfeI C'AATT_G
Cuts at: 2814 2814
Size: 5215
MluI A'CGCG_T
Cuts at: 5091 5091
Size: 5215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 4329 4513 1305
Size: 1026 1998 184 2007
Fragments arranged by size:
2007 1998 1026 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3393
Fragments arranged by size:
3393 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2357 3173 3532 3691 518
Size: 648 1155 36 816 359 159 2042
Fragments arranged by size:
2042 1155 816 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2386 2631 3308 4456 4701 1606
Size: 780 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 677 245 245
NcoI C'CATG_G
Cuts at: 513 1065 2124 513
Size: 552 1059 3604
Fragments arranged by size:
3604 1059 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5215
NotI GC'GGCC_GC
Cuts at: 2662 2662
Size: 5215
NspI r_CATG'y
Cuts at: 1132 1229 2146 2573 4118 1132
Size: 97 917 427 1545 2229
Fragments arranged by size:
2229 1545 917 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5215
PciI A'CATG_T
Cuts at: 4114 4114
Size: 5215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5215
PmeI GTTT'AAAC
Cuts at: 2519 2519
Size: 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 4797 4829 3284
Size: 32 1481 32 3670
Fragments arranged by size:
3670 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2116
Size: 5215
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5215
PsiI TTA'TAA
Cuts at: 2785 2785
Size: 5215
PstI C_TGCA'G
Cuts at: 830 2567 830
Size: 1737 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5215
PvuII CAG'CTG
Cuts at: 2386 2386
Size: 5215
SalI G'TCGA_C
Cuts at: 1936 2557 1936
Size: 621 4594
SbfI CC_TGCA'GG
Cuts at: 2567 2567
Size: 5215
ScaI AGT'ACT
Cuts at: 3463 5175 3463
Size: 1712 3503
SfcI C'TryA_G
Cuts at: 826 1045 2563 3698 4379 4570 826
Size: 219 1518 1135 681 191 1471
Fragments arranged by size:
1518 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5215
SmaI CCC'GGG
Cuts at: 1872 2542 1872
Size: 670 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4220 4482 820
Size: 197 926 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3006 493
Size: 2513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5215
SphI G_CATG'C
Cuts at: 1132 2573 1132
Size: 1441 3774
SspI AAT'ATT
Cuts at: 5 52 3139 5
Size: 47 3087 2081
Fragments arranged by size:
3087 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5215
StyI C'CwwG_G
Cuts at: 513 1065 2124 2236 2654 513
Size: 552 1059 112 418 3074
Fragments arranged by size:
3074 1059 552 418 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3242 3259 3412 3597 4042 325
Size: 1255 1662 17 153 185 445 1498
Fragments arranged by size:
1662 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3461 5173 96
Size: 274 80 33 51 1443 1484 1712 138
Fragments arranged by size:
1712 1484 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5175 5207 5175
Size: 32 5183
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5215
XbaI T'CTAG_A
Cuts at: 2551 2551
Size: 5215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5215
XmaI C'CCGG_G
Cuts at: 1870 2540 1870
Size: 670 4545
XmnI GAAnn'nnTTC
Cuts at: 2020 2366 3344 2020
Size: 346 978 3891
Fragments arranged by size:
3891 978 346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2409 3023 276
Size: 53 83 186 936 875 614 2468
Fragments arranged by size:
2468 936 875 614 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI