Restriction map for
pFN21AE6131
(Circular) (Six-base) MAPSORT of: pFN21AE6131.seq Check: 8443 from: 1 to: 4894
pFN21AE6131 4894 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2704 278
Size: 53 83 186 936 1168 2468
Fragments arranged by size:
2468 1168 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2237 1937
Size: 300 4594
Acc65I G'GTAC_C
Cuts at: 2215 2215
Size: 4894
AclI AA'CG_TT
Cuts at: 1563 3021 3394 1563
Size: 1458 373 3063
Fragments arranged by size:
3063 1458 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2957 4341 4609 1632
Size: 1325 1384 268 1917
Fragments arranged by size:
1917 1384 1325 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4697
AflIII A'CryG_T
Cuts at: 1203 3793 4770 1203
Size: 2590 977 1327
Fragments arranged by size:
2590 1327 977
AgeI A'CCGG_T
Cuts at: 3725 3725
Size: 4894
AhdI GACnn_n'nnGTC
Cuts at: 3623 3623
Size: 4894
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4894
AlwNI CAG_nnn'CTG
Cuts at: 2637 4209 2637
Size: 1572 3322
ApaI G_GGCC'C
Cuts at: 2072 2072
Size: 4894
ApaLI G'TGCA_C
Cuts at: 2951 4107 2951
Size: 1156 3738
ApoI r'AATT_y
Cuts at: 1493 2080 2203 2429 2581 4690 1493
Size: 587 123 226 152 2109 1697
Fragments arranged by size:
2109 1697 587 226 152 123
AseI AT'TA_AT
Cuts at: 160 1032 3448 160
Size: 872 2416 1606
Fragments arranged by size:
2416 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4894
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 1870
Size: 73 276 4545
Fragments arranged by size:
4545 276 73
BamHI G'GATC_C
Cuts at: 2224 2591 2224
Size: 367 4527
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 3670 618
Size: 325 217 627 428 1455 1842
Fragments arranged by size:
1842 1455 627 428 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2213 1391
Size: 681 141 4072
Fragments arranged by size:
4072 681 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3085 3119 1903
Size: 34 1148 34 3678
Fragments arranged by size:
3678 1148 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2786 3996 2786
Size: 1210 3684
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4894
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3505 136
Size: 107 122 71 631 605 1833 1525
Fragments arranged by size:
1833 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4889 4889
Size: 4894
BlpI GC'TnA_GC
Cuts at: 2311 2311
Size: 4894
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2955 4111 1790
Size: 282 883 1156 2573
Fragments arranged by size:
2573 1156 883 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4894
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2625 3583 442
Size: 416 53 83 399 24 1208 958 1753
Fragments arranged by size:
1753 1208 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3554 1239
Size: 2315 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3019 3884 4182 3019
Size: 865 298 3731
Fragments arranged by size:
3731 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3557 882
Size: 617 2058 2219
Fragments arranged by size:
2219 2058 617
BsaAI yAC'GTr
Cuts at: 493 1204 2685 493
Size: 711 1481 2702
Fragments arranged by size:
2702 1481 711
BsaBI GATnn'nnATC
Cuts at: 4888 4888
Size: 4894
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2701 3083 3718 275
Size: 53 83 186 936 1168 382 635 1451
Fragments arranged by size:
1451 1168 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2022 3327 3725 3999 4146 4557 2022
Size: 1305 398 274 147 411 2359
Fragments arranged by size:
2359 1305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4669
Fragments arranged by size:
4669 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 4002
BseYI C'CCAG_C
Cuts at: 4097 4593 4097
Size: 496 4398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3105 4133 1540
Size: 275 186 343 761 1028 2301
Fragments arranged by size:
2301 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2955 3040 4111 2955
Size: 85 1071 3738
Fragments arranged by size:
3738 1071 85
BsmI GAATG_Cn'
Cuts at: 2405 2498 2405
Size: 93 4801
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2213 2955 3040 4111 1391
Size: 399 282 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 141 85
BspEI T'CCGG_A
Cuts at: 2022 4557 2022
Size: 2535 2359
BspHI T'CATG_A
Cuts at: 2783 4513 2783
Size: 1730 3164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2249 844
Size: 1405 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2781 1394
Size: 429 958 3507
Fragments arranged by size:
3507 958 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3389 3563 65
Size: 1183 2141 174 1396
Fragments arranged by size:
2141 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3538 3725 1700
Size: 110 1728 187 2869
Fragments arranged by size:
2869 1728 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4894
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4894
BssSI C'ACGA_G
Cuts at: 2954 3966 2954
Size: 1012 3882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4894
BstBI TT'CG_AA
Cuts at: 3698 3698
Size: 4894
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4894
BstYI r'GATC_y
Cuts at: 1972 2141 2224 2591 2976 2993 4445 4531 4543
Size: 169 83 367 385 17 1452 86 12
Cuts at: 4543 4889 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 169 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4155
Fragments arranged by size:
4155 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 3204 3224 2410
Size: 794 20 4080
Fragments arranged by size:
4080 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2022 2057 529
Size: 35 1458 35 3366
Fragments arranged by size:
3366 1458 35 35
DraI TTT'AAA
Cuts at: 2198 2198
Size: 4894
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3901 809
Size: 507 2585 1802
Fragments arranged by size:
2585 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2341 3230 4859
Size: 54 1425 50 275 18 511 889 1629
Cuts at: 4859 8
Size: 43
Fragments arranged by size:
1629 1425 889 511 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2341 1537
Size: 275 529 4090
Fragments arranged by size:
4090 529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2824 1353
Size: 1471 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3481 3855 4001 387
Size: 1064 138 1892 374 146 1280
Fragments arranged by size:
1892 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2957 3554 4341 4609 1239
Size: 393 1325 597 787 268 1524
Fragments arranged by size:
1524 1325 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2068 2069 2068
Size: 1 4893
EcoRI G'AATT_C
Cuts at: 2203 2581 2203
Size: 378 4516
FspI TGC'GCA
Cuts at: 3400 3400
Size: 4894
HaeII r_GCGC'y
Cuts at: 1678 4041 1678
Size: 2363 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3535 3567 3609 3641 1373
Size: 32 135 32 1963 32 42 32 2626
Fragments arranged by size:
2626 1963 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2238 2484 669
Size: 988 281 300 246 3079
Fragments arranged by size:
3079 988 300 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4894
HpaI GTT'AAC
Cuts at: 2484 2484
Size: 4894
KpnI G_GTAC'C
Cuts at: 2219 2219
Size: 4894
MfeI C'AATT_G
Cuts at: 2493 2493
Size: 4894
MluI A'CGCG_T
Cuts at: 4770 4770
Size: 4894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4008 4192 1305
Size: 2703 184 2007
Fragments arranged by size:
2703 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3072
Fragments arranged by size:
3072 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2852 3211 3370 518
Size: 648 1686 359 159 2042
Fragments arranged by size:
2042 1686 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2310 2987 4135 4380 1606
Size: 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4342
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4894
NotI GC'GGCC_GC
Cuts at: 2341 2341
Size: 4894
NspI r_CATG'y
Cuts at: 1132 1229 2252 3797 1132
Size: 97 1023 1545 2229
Fragments arranged by size:
2229 1545 1023 97
PasI CC'CwG_GG
Cuts at: 1348 2072 1348
Size: 724 4170
PciI A'CATG_T
Cuts at: 3793 3793
Size: 4894
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4894
PmeI GTTT'AAAC
Cuts at: 2198 2198
Size: 4894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 4476 4508 2963
Size: 32 1481 32 3349
Fragments arranged by size:
3349 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4894
PsiI TTA'TAA
Cuts at: 2464 2464
Size: 4894
PspOMI G'GGCC_C
Cuts at: 2068 2068
Size: 4894
PstI C_TGCA'G
Cuts at: 830 2246 830
Size: 1416 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4894
SalI G'TCGA_C
Cuts at: 1936 2236 1936
Size: 300 4594
SbfI CC_TGCA'GG
Cuts at: 2246 2246
Size: 4894
ScaI AGT'ACT
Cuts at: 3142 4854 3142
Size: 1712 3182
SfcI C'TryA_G
Cuts at: 826 1045 2242 3377 4058 4249 826
Size: 219 1197 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4894
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4894
SmaI CCC'GGG
Cuts at: 1872 2221 1872
Size: 349 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2998 3899 4161 820
Size: 197 926 1055 901 262 1553
Fragments arranged by size:
1553 1055 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2685 493
Size: 2192 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4894
SphI G_CATG'C
Cuts at: 1132 2252 1132
Size: 1120 3774
SspI AAT'ATT
Cuts at: 5 52 2818 5
Size: 47 2766 2081
Fragments arranged by size:
2766 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4894
StyI C'CwwG_G
Cuts at: 513 1065 2333 513
Size: 552 1268 3074
Fragments arranged by size:
3074 1268 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 2938 3091 3276 3721 325
Size: 1255 1341 17 153 185 445 1498
Fragments arranged by size:
1498 1341 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 3140 4852
Size: 274 80 33 51 1443 156 1007 1712
Cuts at: 4852 96
Size: 138
Fragments arranged by size:
1712 1443 1007 274 156 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 4854 4886 2090
Size: 32 2732 32 2098
Fragments arranged by size:
2732 2098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4894
XbaI T'CTAG_A
Cuts at: 2230 2230
Size: 4894
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4894
XmaI C'CCGG_G
Cuts at: 1870 2219 1870
Size: 349 4545
XmnI GAAnn'nnTTC
Cuts at: 3023 3023
Size: 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2702 276
Size: 53 83 186 936 1168 2468
Fragments arranged by size:
2468 1168 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI