Restriction map for
pFN21AE6116
(Circular) (Six-base) MAPSORT of: pFN21AE6116.seq Check: 5345 from: 1 to: 5143
pFN21AE6116 5143 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2953 278
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2486 1937
Size: 549 4594
Acc65I G'GTAC_C
Cuts at: 2464 2464
Size: 5143
AclI AA'CG_TT
Cuts at: 1563 3270 3643 1563
Size: 1707 373 3063
Fragments arranged by size:
3063 1707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3206 4590 4858 1632
Size: 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4946
AflIII A'CryG_T
Cuts at: 1203 4042 5019 1203
Size: 2839 977 1327
Fragments arranged by size:
2839 1327 977
AgeI A'CCGG_T
Cuts at: 3974 3974
Size: 5143
AhdI GACnn_n'nnGTC
Cuts at: 3872 3872
Size: 5143
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 1184
Size: 1095 4048
AlwNI CAG_nnn'CTG
Cuts at: 2886 4458 2886
Size: 1572 3571
ApaLI G'TGCA_C
Cuts at: 2141 3200 4356 2141
Size: 1059 1156 2928
Fragments arranged by size:
2928 1156 1059
ApoI r'AATT_y
Cuts at: 1493 2423 2452 2678 2830 4939 1493
Size: 930 29 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 3697 160
Size: 872 2665 1606
Fragments arranged by size:
2665 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 1870
Size: 73 525 4545
Fragments arranged by size:
4545 525 73
BamHI G'GATC_C
Cuts at: 2473 2840 2473
Size: 367 4776
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2329 2464 3919 618
Size: 325 217 627 285 257 135 1455 1842
Fragments arranged by size:
1842 1455 627 325 285 257 217 135
BanII G_rGCy'C
Cuts at: 1391 2462 1391
Size: 1071 4072
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 5143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4172
BbvCI CC'TCA_GC
Cuts at: 2082 2082
Size: 5143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3334 3368 1903
Size: 34 1397 34 3678
Fragments arranged by size:
3678 1397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2399 3035 4245 2399
Size: 636 1210 3297
Fragments arranged by size:
3297 1210 636
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5143
BfrBI ATG'CAT
Cuts at: 2242 2242
Size: 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3754 136
Size: 107 122 71 631 605 2082 1525
Fragments arranged by size:
2082 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5138 5138
Size: 5143
BlpI GC'TnA_GC
Cuts at: 2560 2560
Size: 5143
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2145 3204 4360 1790
Size: 278 77 1059 1156 2573
Fragments arranged by size:
2573 1156 1059 278 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5143
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3832 442
Size: 416 53 83 399 24 1457 958 1753
Fragments arranged by size:
1753 1457 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3803 1239
Size: 2564 2579
Bpu10I CC'TnA_GC
Cuts at: 2082 2082
Size: 5143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3268 4133 4431 3268
Size: 865 298 3980
Fragments arranged by size:
3980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3806 882
Size: 617 2307 2219
Fragments arranged by size:
2307 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2934 493
Size: 711 1730 2702
Fragments arranged by size:
2702 1730 711
BsaBI GATnn'nnATC
Cuts at: 5137 5137
Size: 5143
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2950 3332 3967
Size: 53 83 186 936 540 877 382 635
Cuts at: 3967 275
Size: 1451
Fragments arranged by size:
1451 936 877 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3576 3974 4248 4395 4806 3576
Size: 398 274 147 411 3913
Fragments arranged by size:
3913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4918
Fragments arranged by size:
4918 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4230
BseYI C'CCAG_C
Cuts at: 4346 4842 4346
Size: 496 4647
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2593 3354 4382 1540
Size: 275 186 592 761 1028 2301
Fragments arranged by size:
2301 1028 761 592 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 3204 3289 4360 2145
Size: 1059 85 1071 2928
Fragments arranged by size:
2928 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2654 2747 2654
Size: 93 5050
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2145 2462 3204 3289 4360 1391
Size: 399 278 77 317 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 317 278 85 77
BspEI T'CCGG_A
Cuts at: 4806 4806
Size: 5143
BspHI T'CATG_A
Cuts at: 3032 4762 3032
Size: 1730 3413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 2498 844
Size: 1587 67 3489
Fragments arranged by size:
3489 1587 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3030 1394
Size: 429 1207 3507
Fragments arranged by size:
3507 1207 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3638 3812 65
Size: 1183 2390 174 1396
Fragments arranged by size:
2390 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3787 3974 1700
Size: 110 1977 187 2869
Fragments arranged by size:
2869 1977 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5143
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5143
BssSI C'ACGA_G
Cuts at: 3203 4215 3203
Size: 1012 4131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5143
BstBI TT'CG_AA
Cuts at: 3947 3947
Size: 5143
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5143
BstYI r'GATC_y
Cuts at: 1972 2015 2473 2840 3225 3242 4694 4780 4792
Size: 43 458 367 385 17 1452 86 12
Cuts at: 4792 5138 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 458 385 367 346 86 43
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4591
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4404
Fragments arranged by size:
4404 683 56
BtsI GCAGTG_nn'
Cuts at: 2435 2659 3453 3473 2435
Size: 224 794 20 4105
Fragments arranged by size:
4105 794 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2199 2234 529
Size: 35 1635 35 3438
Fragments arranged by size:
3438 1635 35 35
DraI TTT'AAA
Cuts at: 2447 2447
Size: 5143
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4150 809
Size: 507 2834 1802
Fragments arranged by size:
2834 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2590 3479 5108
Size: 54 1425 50 275 18 760 889 1629
Cuts at: 5108 8
Size: 43
Fragments arranged by size:
1629 1425 889 760 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2590 1537
Size: 275 778 4090
Fragments arranged by size:
4090 778 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3073 1353
Size: 1720 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 3730 4104 4250 387
Size: 1064 138 624 1517 374 146 1280
Fragments arranged by size:
1517 1280 1064 624 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3206 3803 4590 4858 1239
Size: 393 1574 597 787 268 1524
Fragments arranged by size:
1574 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2452 2830 2452
Size: 378 4765
FspI TGC'GCA
Cuts at: 3649 3649
Size: 5143
HaeII r_GCGC'y
Cuts at: 1678 2076 4290 1678
Size: 398 2214 2531
Fragments arranged by size:
2531 2214 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 3784 3816 3858
Size: 32 135 32 435 32 1745 32 42
Cuts at: 3858 3890 1373
Size: 32 2626
Fragments arranged by size:
2626 1745 435 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2487 2733 669
Size: 988 281 549 246 3079
Fragments arranged by size:
3079 988 549 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5143
HpaI GTT'AAC
Cuts at: 2733 2733
Size: 5143
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 5143
KpnI G_GTAC'C
Cuts at: 2468 2468
Size: 5143
MfeI C'AATT_G
Cuts at: 2742 2742
Size: 5143
MluI A'CGCG_T
Cuts at: 5019 5019
Size: 5143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4257 4441 1305
Size: 2952 184 2007
Fragments arranged by size:
2952 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3321
Fragments arranged by size:
3321 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2247 3101 3460 3619 518
Size: 648 843 238 854 359 159 2042
Fragments arranged by size:
2042 854 843 648 359 238 159
MspA1I CmG'CkG
Cuts at: 1606 2559 3236 4384 4629 1606
Size: 953 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 245
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 5143
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4591
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5143
NotI GC'GGCC_GC
Cuts at: 2590 2590
Size: 5143
NsiI A_TGCA'T
Cuts at: 2244 2244
Size: 5143
NspI r_CATG'y
Cuts at: 1132 1229 2501 4046 1132
Size: 97 1272 1545 2229
Fragments arranged by size:
2229 1545 1272 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5143
PciI A'CATG_T
Cuts at: 4042 4042
Size: 5143
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5143
PmeI GTTT'AAAC
Cuts at: 2447 2447
Size: 5143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 4725 4757 3212
Size: 32 1481 32 3598
Fragments arranged by size:
3598 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5143
PsiI TTA'TAA
Cuts at: 2713 2713
Size: 5143
PstI C_TGCA'G
Cuts at: 830 2095 2442 2495 830
Size: 1265 347 53 3478
Fragments arranged by size:
3478 1265 347 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5143
SalI G'TCGA_C
Cuts at: 1936 2485 1936
Size: 549 4594
SbfI CC_TGCA'GG
Cuts at: 2495 2495
Size: 5143
ScaI AGT'ACT
Cuts at: 3391 5103 3391
Size: 1712 3431
SfcI C'TryA_G
Cuts at: 826 1045 2091 2438 2491 3626 4307 4498 826
Size: 219 1046 347 53 1135 681 191 1471
Fragments arranged by size:
1471 1135 1046 681 347 219 191 53
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 5143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5143
SmaI CCC'GGG
Cuts at: 1872 2470 1872
Size: 598 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3247 4148 4410 820
Size: 197 926 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2934 493
Size: 2441 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5143
SphI G_CATG'C
Cuts at: 1132 2501 1132
Size: 1369 3774
SspI AAT'ATT
Cuts at: 5 52 3067 5
Size: 47 3015 2081
Fragments arranged by size:
3015 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5143
StyI C'CwwG_G
Cuts at: 513 1065 2582 513
Size: 552 1517 3074
Fragments arranged by size:
3074 1517 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 3187 3340 3525 3970 325
Size: 1255 1590 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3389 5101 96
Size: 274 80 33 51 1443 1412 1712 138
Fragments arranged by size:
1712 1443 1412 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5103 5135 5103
Size: 32 5111
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5143
XbaI T'CTAG_A
Cuts at: 2170 2479 2170
Size: 309 4834
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5143
XmaI C'CCGG_G
Cuts at: 1870 2468 1870
Size: 598 4545
XmnI GAAnn'nnTTC
Cuts at: 3272 3272
Size: 5143
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2951 276
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI