Restriction map for
pFN21AE6096
(Circular) (Six-base) MAPSORT of: pFN21AE6096.seq Check: 2814 from: 1 to: 5683
pFN21AE6096 5683 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 2678 3004 2678
Size: 326 5357
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2724 3746 5130 5398 1632
Size: 467 625 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1022 625 467 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2116 4582 5559 1203
Size: 913 2466 977 1327
Fragments arranged by size:
2466 1327 977 913
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2531 2666 3426 4998 2531
Size: 135 760 1572 3216
Fragments arranged by size:
3216 1572 760 135
ApaLI G'TGCA_C
Cuts at: 2244 3740 4896 2244
Size: 1496 1156 3031
Fragments arranged by size:
3031 1496 1156
ApoI r'AATT_y
Cuts at: 1493 2037 2084 2639 2992 3218 3370 5479 1493
Size: 544 47 555 353 226 152 2109 1697
Fragments arranged by size:
2109 1697 555 544 353 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 2733 3013 3380 2733
Size: 280 367 5036
Fragments arranged by size:
5036 367 280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 3004 4459 618
Size: 325 217 627 891 326 1455 1842
Fragments arranged by size:
1842 1455 891 627 326 325 217
BanII G_rGCy'C
Cuts at: 1391 3002 1391
Size: 1611 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2701 928
Size: 971 235 567 3910
Fragments arranged by size:
3910 971 567 235
BbvCI CC'TCA_GC
Cuts at: 2745 2897 2745
Size: 152 5531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2181 1548
Size: 633 5050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2302 5678 2302
Size: 3376 2307
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2248 3744 4900 1790
Size: 458 1496 1156 2573
Fragments arranged by size:
2573 1496 1156 458
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4343 1239
Size: 3104 2579
Bpu10I CC'TnA_GC
Cuts at: 2745 2897 2745
Size: 152 5531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 4346 882
Size: 617 756 2091 2219
Fragments arranged by size:
2219 2091 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 2060 5677 2060
Size: 3617 2066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5683
BseYI C'CCAG_C
Cuts at: 2674 4886 5382 2674
Size: 2212 496 2975
Fragments arranged by size:
2975 2212 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2517 1163
Size: 573 781 4329
Fragments arranged by size:
4329 781 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2248 3744 3829 4900 2248
Size: 1496 85 1071 3031
Fragments arranged by size:
3031 1496 1071 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 3002 3744 3829 4900 1391
Size: 399 458 754 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 458 399 85
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2162 2273 3038 844
Size: 1318 111 765 3489
Fragments arranged by size:
3489 1318 765 111
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 2119 96
Size: 2023 3660
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2302 2733 3013 3380 3765 3782 5234 5320
Size: 330 431 280 367 385 17 1452 86
Cuts at: 5320 5332 5678 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 431 385 367 346 330 280
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2729 2845 2918 2729
Size: 116 73 5494
Fragments arranged by size:
5494 116 73
BtgI C'CryG_G
Cuts at: 513 1065 2004 2790 513
Size: 552 939 786 3406
Fragments arranged by size:
3406 939 786 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2450 2499 3199 3993 4013 2450
Size: 49 700 794 20 4120
Fragments arranged by size:
4120 794 700 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2424 3130
Size: 54 1425 50 275 18 480 114 706
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 706 480 275 114 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3613 1353
Size: 887 1373 3423
Fragments arranged by size:
3423 1373 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2724 3746 4343 5130 5398 1239
Size: 393 467 625 1022 597 787 268 1524
Fragments arranged by size:
1524 1022 787 625 597 467 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2211 2211
Size: 5683
EcoRI G'AATT_C
Cuts at: 2084 2639 2992 3370 2084
Size: 555 353 378 4397
Fragments arranged by size:
4397 555 378 353
EcoRV GAT'ATC
Cuts at: 2447 2447
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373
Size: 32 135 32 2752 32 42 32 2626
Fragments arranged by size:
2752 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 2682 3008 2682
Size: 326 5357
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 2426 10
Size: 54 1425 343 480 114 3267
Fragments arranged by size:
3267 1425 480 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2402 3641 4000 4159 518
Size: 648 1059 177 1239 359 159 2042
Fragments arranged by size:
2042 1239 1059 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2744 3099 3776 4924 5169 1606
Size: 1138 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 1138 677 355 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 2592 387
Size: 2205 3478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2120 3041 4586 1132
Size: 97 891 921 1545 2229
Fragments arranged by size:
2229 1545 921 891 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 2116 4582 2116
Size: 2466 3217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 1108
Size: 1488 4195
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2211 2211
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 2282 2705 1613
Size: 669 423 4591
Fragments arranged by size:
4591 669 423
PsiI TTA'TAA
Cuts at: 2648 2798 3253 2648
Size: 150 455 5078
Fragments arranged by size:
5078 455 150
PstI C_TGCA'G
Cuts at: 830 2506 2561 3035 830
Size: 1676 55 474 3478
Fragments arranged by size:
3478 1676 474 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2744 2744
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2502 2557 3031 4166 4847 5038 826
Size: 219 1457 55 474 1135 681 191 1471
Fragments arranged by size:
1471 1457 1135 681 474 219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4438
StyI C'CwwG_G
Cuts at: 513 1065 2004 2313 3122 513
Size: 552 939 309 809 3074
Fragments arranged by size:
3074 939 809 552 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 3710 3727 3880 4065 4510 325
Size: 1255 611 1519 17 153 185 445 1498
Fragments arranged by size:
1519 1498 1255 611 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2119 3929 5641
Size: 274 80 33 51 1443 142 1810 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1810 1712 1443 274 142 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 5643 5675 2469
Size: 32 3142 32 2477
Fragments arranged by size:
3142 2477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 2088 2326 2762 3812 2084
Size: 4 238 436 1050 3955
Fragments arranged by size:
3955 1050 436 238 4
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI