Restriction map for
pFN21AE6094
(Circular) (Six-base) MAPSORT of: pFN21AE6094.seq Check: 9905 from: 1 to: 5683
pFN21AE6094 5683 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2483 2780 2483
Size: 297 5386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 3026 1937
Size: 562 527 4594
Fragments arranged by size:
4594 562 527
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2749 3746 5130 5398 1632
Size: 1117 997 1384 268 1917
Fragments arranged by size:
1917 1384 1117 997 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 2171 1166
Size: 1005 4678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2772 3426 4998 2772
Size: 654 1572 3457
Fragments arranged by size:
3457 1572 654
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 3008 1870
Size: 73 207 858 4545
Fragments arranged by size:
4545 858 207 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4459 618
Size: 325 217 627 1217 1455 1842
Fragments arranged by size:
1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3002 1391
Size: 1611 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2641 928
Size: 971 742 3970
Fragments arranged by size:
3970 971 742
BbvCI CC'TCA_GC
Cuts at: 2966 2966
Size: 5683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 5041
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 3744 4900 1790
Size: 1954 1156 2573
Fragments arranged by size:
2573 1954 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2138 2138
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2704 4343 1239
Size: 869 596 1639 2579
Fragments arranged by size:
2579 1639 869 596
Bpu10I CC'TnA_GC
Cuts at: 2966 2966
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 3808 4673 4971 2081
Size: 1727 865 298 2793
Fragments arranged by size:
2793 1727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4516
Fragments arranged by size:
4516 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2132 2266 1186
Size: 879 67 134 4603
Fragments arranged by size:
4603 879 134 67
BseYI C'CCAG_C
Cuts at: 4886 5382 4886
Size: 496 5187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2975 1163
Size: 573 468 771 3871
Fragments arranged by size:
3871 771 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3133 3894 4922 1540
Size: 275 186 418 714 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 3744 3829 4900 2568
Size: 1176 85 1071 3351
Fragments arranged by size:
3351 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 3194 3287 2819
Size: 375 93 5215
Fragments arranged by size:
5215 375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2568 3002 3744 3829 4900 1391
Size: 399 778 434 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 434 399 85
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 2604 3572 5302 2604
Size: 968 1730 2985
Fragments arranged by size:
2985 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 2780 3038 844
Size: 1639 297 258 3489
Fragments arranged by size:
3489 1639 297 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2379 4178 4352 65
Size: 1183 1131 1799 174 1396
Fragments arranged by size:
1799 1396 1183 1131 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 2680 3743 4755 2680
Size: 1063 1012 3608
Fragments arranged by size:
3608 1063 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2164 1184
Size: 980 4703
BstYI r'GATC_y
Cuts at: 1972 2154 2263 2753 3013 3380 3765 3782 5234
Size: 182 109 490 260 367 385 17 1452
Cuts at: 5234 5320 5332 5678 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 490 385 367 346 260 182
109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 2927
Size: 5683
BtgI C'CryG_G
Cuts at: 513 1065 2004 2793 2889 513
Size: 552 939 789 96 3307
Fragments arranged by size:
3307 939 789 552 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2150 3613 1353
Size: 752 45 1463 3423
Fragments arranged by size:
3423 1463 752 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2704 2749 3746 4343 5130 5398
Size: 393 476 596 45 997 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 997 787 597 596 476 393 268
45
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2655 2687 2738 2770 4324
Size: 32 135 32 1083 32 51 32 1554
Cuts at: 4324 4356 4398 4430 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1554 1083 135 51 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2228 2459 3641 4000 4159 518
Size: 648 1005 57 231 1182 359 159 2042
Fragments arranged by size:
2042 1182 1005 648 359 231 159 57
MspA1I CmG'CkG
Cuts at: 1606 2218 3099 3776 4924 5169 1606
Size: 612 881 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 677 612 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2350 2398 3041 4586 1132
Size: 97 1121 48 643 1545 2229
Fragments arranged by size:
2229 1545 1121 643 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2036 2065 2777 3035 830
Size: 1206 29 712 258 3478
Fragments arranged by size:
3478 1206 712 258 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2218 2218
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 2036 3035 2036
Size: 999 4684
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2032 2061 2076 2773 2920 3031 4166
Size: 219 987 29 15 697 147 111 1135
Cuts at: 4166 4847 5038 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 987 697 681 219 191 147
111 29 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 3787 4688 4950 820
Size: 197 926 153 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2038 1517
Size: 521 5162
StyI C'CwwG_G
Cuts at: 513 1065 2004 3122 513
Size: 552 939 1118 3074
Fragments arranged by size:
3074 1118 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3710 3727 3880 4065 4510 325
Size: 1255 480 1650 17 153 185 445 1498
Fragments arranged by size:
1650 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 2826 3929
Size: 274 80 33 51 1443 255 594 1103
Cuts at: 3929 5641 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1103 594 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI