Restriction map for
pFN21AE6086
(Circular) (Six-base) MAPSORT of: pFN21AE6086.seq Check: 6994 from: 1 to: 5677
pFN21AE6086 5677 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2183 2222 3020 1937
Size: 246 39 798 4594
Fragments arranged by size:
4594 798 246 39
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2726 4576 5553 1203
Size: 1523 1850 977 1327
Fragments arranged by size:
1850 1523 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 4453 618
Size: 325 217 627 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2996 1391
Size: 624 981 4072
Fragments arranged by size:
4072 981 624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 3408 4366
Size: 416 53 83 399 24 1126 865 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1126 958 865 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2721 4337 1239
Size: 992 490 1616 2579
Fragments arranged by size:
2579 1616 992 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2947 3802 4667 4965 2947
Size: 855 865 298 3659
Fragments arranged by size:
3659 865 855 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2687 2727 3468 493
Size: 711 1483 40 741 2702
Fragments arranged by size:
2702 1483 741 711 40
BsaBI GATnn'nnATC
Cuts at: 2645 5671 2645
Size: 3026 2651
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4621
Fragments arranged by size:
4621 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 1186
Size: 1098 4579
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2551 1163
Size: 573 815 4289
Fragments arranged by size:
4289 815 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2200 3738 3823 4894 2200
Size: 1538 85 1071 2983
Fragments arranged by size:
2983 1538 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2200 2996 3738 3823 4894 1391
Size: 399 225 185 796 742 85 1071 2174
Fragments arranged by size:
2174 1071 796 742 399 225 185 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2406 2589 3566 5296 2406
Size: 183 977 1730 2787
Fragments arranged by size:
2787 1730 977 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4172 4346 65
Size: 1183 1578 1346 174 1396
Fragments arranged by size:
1578 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 4321 4508 1700
Size: 110 511 2000 187 2869
Fragments arranged by size:
2869 2000 511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2577 3737 4749 2577
Size: 1160 1012 3505
Fragments arranged by size:
3505 1160 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 2513 4481 2513
Size: 1968 3709
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2487 3007 3374 3759 3776 5228 5314 5326
Size: 515 520 367 385 17 1452 86 12
Cuts at: 5326 5672 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 520 515 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2184 2223 2184
Size: 39 5638
Bsu36I CC'TnA_GG
Cuts at: 2882 2882
Size: 5677
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2093 2495 2625 2773 3193 3987 4007 2093
Size: 402 130 148 420 794 20 3763
Fragments arranged by size:
3763 794 420 402 148 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 3124 4013
Size: 54 1425 50 275 18 543 751 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 751 543 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2394 2762 3607 1353
Size: 752 289 368 845 3423
Fragments arranged by size:
3423 845 752 368 289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2721 3740 4337 5124 5392 1239
Size: 393 599 490 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 787 599 597 490 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2958 2958
Size: 5677
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2571 2603 4318
Size: 32 135 32 430 32 537 32 1715
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1715 537 430 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 4791 4975 1305
Size: 799 2687 184 2007
Fragments arranged by size:
2687 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3312
Fragments arranged by size:
3312 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2177 2588 2651 3635 3994 4153
Size: 648 978 33 411 63 984 359 159
Cuts at: 4153 518
Size: 2042
Fragments arranged by size:
2042 984 978 648 411 359 159 63
33
MspA1I CmG'CkG
Cuts at: 1606 2636 2699 3093 3770 4918 5163 1606
Size: 1030 63 394 677 1148 245 2120
Fragments arranged by size:
2120 1148 1030 677 394 245 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
NdeI CA'TA_TG
Cuts at: 387 2378 387
Size: 1991 3686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 2507 1108
Size: 1263 136 4278
Fragments arranged by size:
4278 1263 136
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PmlI CAC'GTG
Cuts at: 2727 2727
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2958 2958
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2301 3029 830
Size: 1471 728 3478
Fragments arranged by size:
3478 1471 728
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2636 2699 2636
Size: 63 5614
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SapI GCTCTTCn'nnn_
Cuts at: 2105 2762 2105
Size: 657 5020
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SexAI A'CCwGG_T
Cuts at: 2130 2130
Size: 5677
SfcI C'TryA_G
Cuts at: 826 1045 2297 2875 3025 4160 4841 5032 826
Size: 219 1252 578 150 1135 681 191 1471
Fragments arranged by size:
1471 1252 1135 681 578 219 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2962 3781 4682 4944 820
Size: 197 926 1019 819 901 262 1553
Fragments arranged by size:
1553 1019 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 2687 3468 493
Size: 2194 781 2702
Fragments arranged by size:
2702 2194 781
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2004 3116 513
Size: 552 939 1112 3074
Fragments arranged by size:
3074 1112 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 5637 5669 2455
Size: 32 3150 32 2463
Fragments arranged by size:
3150 2463 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI