Restriction map for
pFN21AE6085
(Circular) (Six-base) MAPSORT of: pFN21AE6085.seq Check: 7529 from: 1 to: 5677
pFN21AE6085 5677 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2771 2771
Size: 5677
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2778 1166
Size: 1612 4065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 5645
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 4453 618
Size: 325 217 627 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 2817 2996 1391
Size: 826 600 179 4072
Fragments arranged by size:
4072 826 600 179
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2785 928
Size: 971 886 3820
Fragments arranged by size:
3820 971 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3868 3902 1903
Size: 34 50 34 1847 34 3678
Fragments arranged by size:
3678 1847 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2099 3569 4779 2099
Size: 1470 1210 2997
Fragments arranged by size:
2997 1470 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2934 5672 2934
Size: 2738 2939
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4337 1239
Size: 3098 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 2732 3802 4667 4965 2516
Size: 216 1070 865 298 3228
Fragments arranged by size:
3228 1070 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 2306 5671 2306
Size: 3365 2312
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 4519
Fragments arranged by size:
4519 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2254 2843 1186
Size: 1068 589 4020
Fragments arranged by size:
4020 1068 589
BseYI C'CCAG_C
Cuts at: 2016 2833 4880 5376 2016
Size: 817 2047 496 2317
Fragments arranged by size:
2317 2047 817 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 1163
Size: 573 831 4273
Fragments arranged by size:
4273 831 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2817 2996 3738 3823 4894 1391
Size: 399 427 600 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 427 399 179 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 3032 844
Size: 1927 261 3489
Fragments arranged by size:
3489 1927 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 4172 4346 65
Size: 1183 1181 1743 174 1396
Fragments arranged by size:
1743 1396 1183 1181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 2165 1184
Size: 981 4696
BstYI r'GATC_y
Cuts at: 1972 2934 3007 3374 3759 3776 5228 5314 5326
Size: 962 73 367 385 17 1452 86 12
Cuts at: 5326 5672 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 962 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2370 2394 513
Size: 552 1305 24 3796
Fragments arranged by size:
3796 1305 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2516 3193 3987 4007 2516
Size: 677 794 20 4186
Fragments arranged by size:
4186 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DraIII CAC_nnn'GTG
Cuts at: 2969 2969
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4684 809
Size: 507 1305 2063 1802
Fragments arranged by size:
2063 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2232 2286
Size: 54 1425 50 275 18 324 78 54
Cuts at: 2286 2391 2415 2466 3124 4013 5642 8
Size: 105 24 51 658 889 1629 43
Fragments arranged by size:
1629 1425 889 658 324 275 105 78
54 54 51 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2648 2684 3607 1353
Size: 878 417 36 923 3423
Fragments arranged by size:
3423 923 878 417 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3740 4337 5124 5392 1239
Size: 393 2108 597 787 268 1524
Fragments arranged by size:
2108 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2734 2734
Size: 5677
EcoO109I rG'GnC_Cy
Cuts at: 2102 2947 2102
Size: 845 4832
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2275 2307 2631 2663 4318
Size: 32 135 32 703 32 324 32 1655
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1655 703 324 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2902 3021 3267 669
Size: 988 281 964 119 246 3079
Fragments arranged by size:
3079 988 964 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 2234 2288 2393 2417
Size: 54 1425 343 324 78 54 105 24
Cuts at: 2417 2468 10
Size: 51 3219
Fragments arranged by size:
3219 1425 343 324 105 78 54 54
51 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2666 2778 3635 3994 4153 518
Size: 648 1026 474 112 857 359 159 2042
Fragments arranged by size:
2042 1026 857 648 474 359 159 112
MspA1I CmG'CkG
Cuts at: 1606 2381 3093 3770 4918 5163 1606
Size: 775 712 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 712 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2394 513
Size: 552 1329 3796
Fragments arranged by size:
3796 1329 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 4864
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 1108
Size: 915 4762
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 3746 3778 5259 5291 2306
Size: 32 1408 32 1481 32 2692
Fragments arranged by size:
2692 1481 1408 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2947 2947
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 2177 1613
Size: 564 5113
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2381 2381
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2895 3025 4160 4841 5032 826
Size: 219 1850 130 1135 681 191 1471
Fragments arranged by size:
1850 1471 1135 681 219 191 130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 2747 3781 4682 4944 820
Size: 197 926 588 216 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 588 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2151 2394 3116 513
Size: 552 1086 243 722 3074
Fragments arranged by size:
3074 1086 722 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2442 2654 2825 3704 3721 3874 4059
Size: 1255 862 212 171 879 17 153 185
Cuts at: 4059 4504 325
Size: 445 1498
Fragments arranged by size:
1498 1255 879 862 445 212 185 171
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2459 2609 3923
Size: 274 80 33 51 1443 482 150 1314
Cuts at: 3923 5635 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1314 482 274 150 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 2255 1316
Size: 939 4738
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2626 2281
Size: 345 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI