Restriction map for
pFN21AE6084
(Circular) (Six-base) MAPSORT of: pFN21AE6084.seq Check: 999 from: 1 to: 5677
pFN21AE6084 5677 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2400 2998 2400
Size: 598 5079
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 3740 5124 5392 1632
Size: 950 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1158 950 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2580 2726 4576 5553 1203
Size: 1377 146 1850 977 1327
Fragments arranged by size:
1850 1377 1327 977 146
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 2263 4406 2263
Size: 2143 3534
AleI CACnn'nnGTG
Cuts at: 1166 2783 1166
Size: 1617 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2917 2949 2917
Size: 32 5645
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2339 2986 3212 3364 5473 1493
Size: 846 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 846 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2520 3002 1870
Size: 73 135 442 482 4545
Fragments arranged by size:
4545 482 442 135 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2215 2248 2215
Size: 33 5644
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2400 2998 4453 618
Size: 325 217 627 613 598 1455 1842
Fragments arranged by size:
1842 1455 627 613 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2996 1391
Size: 1605 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2012 2092 928
Size: 971 113 80 4513
Fragments arranged by size:
4513 971 113 80
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 5677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2209 3569 4779 2209
Size: 1360 1210 3107
Fragments arranged by size:
3107 1360 1210
BclI T'GATC_A
Cuts at: 1548 2964 1548
Size: 1416 4261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2307 5672 2307
Size: 3365 2312
BlpI GC'TnA_GC
Cuts at: 2195 3094 2195
Size: 899 4778
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 2583 1318
Size: 1265 4412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2009 2237 2793 4337 1239
Size: 770 228 556 1544 2579
Fragments arranged by size:
2579 1544 770 556 228
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 3802 4667 4965 2902
Size: 900 865 298 3614
Fragments arranged by size:
3614 900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 2588 4340 882
Size: 617 861 228 1752 2219
Fragments arranged by size:
2219 1752 861 617 228
BsaAI yAC'GTr
Cuts at: 493 1204 2727 3468 493
Size: 711 1523 741 2702
Fragments arranged by size:
2702 1523 741 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 2917 2947 1178
Size: 30 165 30 744 30 740 30 3908
Fragments arranged by size:
3908 744 740 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4464
BseYI C'CCAG_C
Cuts at: 2350 2551 4880 5376 2350
Size: 201 2329 496 2651
Fragments arranged by size:
2651 2329 496 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 1163
Size: 573 583 4521
Fragments arranged by size:
4521 583 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2996 3738 3823 4894 1391
Size: 399 1206 742 85 1071 2174
Fragments arranged by size:
2174 1206 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2139 2412 3566 5296 2139
Size: 273 1154 1730 2520
Fragments arranged by size:
2520 1730 1154 273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2706 4321 4508 1700
Size: 110 896 1615 187 2869
Fragments arranged by size:
2869 1615 896 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2427 794
Size: 1633 4044
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2113 2011
Size: 102 5575
BstYI r'GATC_y
Cuts at: 1972 2307 2516 2547 3007 3374 3759 3776 5228
Size: 335 209 31 460 367 385 17 1452
Cuts at: 5228 5314 5326 5672 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 460 385 367 346 335 209
86 31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642
Size: 54 1425 50 275 18 1294 889 1629
Cuts at: 5642 8
Size: 43
Fragments arranged by size:
1629 1425 1294 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2237 2582 2793 3740 4337 5124
Size: 393 377 228 345 211 947 597 787
Cuts at: 5124 5392 1239
Size: 268 1524
Fragments arranged by size:
1524 947 787 597 393 377 345 268
228 211
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5677
EcoO109I rG'GnC_Cy
Cuts at: 2257 2257
Size: 5677
EcoRI G'AATT_C
Cuts at: 2339 2986 3364 2339
Size: 647 378 4652
Fragments arranged by size:
4652 647 378
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4318 4350 4392
Size: 32 135 32 677 32 2037 32 42
Cuts at: 4392 4424 1373
Size: 32 2626
Fragments arranged by size:
2626 2037 677 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 2404 3002 2404
Size: 598 5079
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2111 2156 2180 2744 2783 2855
Size: 648 843 102 45 24 564 39 72
Cuts at: 2855 2951 3635 3994 4153 518
Size: 96 684 359 159 2042
Fragments arranged by size:
2042 843 684 648 564 359 159 102
96 72 45 39 24
MspA1I CmG'CkG
Cuts at: 1606 3093 3770 4918 5163 1606
Size: 1487 677 1148 245 2120
Fragments arranged by size:
2120 1487 1148 677 245
NaeI GCC'GGC
Cuts at: 2708 2708
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4186
Fragments arranged by size:
4186 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NgoMIV G'CCGG_C
Cuts at: 2706 2706
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 1108
Size: 1635 4042
PfoI T'CCnGG_A
Cuts at: 2253 2253
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2949 3746 3778 5259 5291 2917
Size: 32 797 32 1481 32 3303
Fragments arranged by size:
3303 1481 797 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2257 2257
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 2489 1613
Size: 876 4801
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2905 3029 830
Size: 2075 124 3478
Fragments arranged by size:
3478 2075 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 2919 3925 5637 2919
Size: 1006 1712 2959
Fragments arranged by size:
2959 1712 1006
SfcI C'TryA_G
Cuts at: 826 1045 2512 2875 2901 3025 4160 4841 5032
Size: 219 1467 363 26 124 1135 681 191
Cuts at: 5032 826
Size: 1471
Fragments arranged by size:
1471 1467 1135 681 363 219 191 124
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2080 3004 1872
Size: 208 924 4545
Fragments arranged by size:
4545 924 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2881 3781 4682 4944 820
Size: 197 926 938 900 901 262 1553
Fragments arranged by size:
1553 938 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2055 1517
Size: 538 5139
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2466 3116 513
Size: 552 939 264 198 650 3074
Fragments arranged by size:
3074 939 650 552 264 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2194 3704 3721 3874 4059 4504
Size: 1255 498 116 1510 17 153 185 445
Cuts at: 4504 325
Size: 1498
Fragments arranged by size:
1510 1498 1255 498 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2917 3923
Size: 274 80 33 51 1443 207 733 1006
Cuts at: 3923 5635 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1006 733 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 5637 5669 2147
Size: 32 3458 32 2155
Fragments arranged by size:
3458 2155 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2287 2113
Size: 174 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2078 3002 1870
Size: 208 924 4545
Fragments arranged by size:
4545 924 208
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI