Restriction map for
pFN21AE6078
(Circular) (Six-base) MAPSORT of: pFN21AE6078.seq Check: 1927 from: 1 to: 5674
pFN21AE6078 5674 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2231 3017 1937
Size: 294 786 4594
Fragments arranged by size:
4594 786 294
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 3737 5121 5389 1632
Size: 887 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 887 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2821 4573 5550 1203
Size: 1618 1752 977 1327
Fragments arranged by size:
1752 1618 1327 977
AgeI A'CCGG_T
Cuts at: 2925 4505 2925
Size: 1580 4094
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2240 2745 1166
Size: 1074 505 4095
Fragments arranged by size:
4095 1074 505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2972 2993 1391
Size: 1581 21 4072
Fragments arranged by size:
4072 1581 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2900 928
Size: 971 1001 3702
Fragments arranged by size:
3702 1001 971
BbvCI CC'TCA_GC
Cuts at: 2723 2723
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2451 2485 3865 3899 1903
Size: 34 514 34 1380 34 3678
Fragments arranged by size:
3678 1380 514 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2247 2395 2619 3735 4891 1790
Size: 394 63 148 224 1116 1156 2573
Fragments arranged by size:
2573 1156 1116 394 224 148 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2477 3405 4363
Size: 416 53 83 399 24 1060 928 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1060 958 928 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 2460
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2723 2723
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2909 3465 493
Size: 711 1705 556 2702
Fragments arranged by size:
2702 1705 711 556
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2925 4107 4505 4779 4926 5337 2925
Size: 1182 398 274 147 411 3262
Fragments arranged by size:
3262 1182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2658 2688 1178
Size: 30 165 30 1255 30 4164
Fragments arranged by size:
4164 1255 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 4877 5373 4877
Size: 496 5178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2247 2395 2619 2972 2993 3735
Size: 399 394 63 148 224 353 21 742
Cuts at: 3735 3820 4891 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 353 224 148
85 63 21
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 4169 4343 65
Size: 1183 1035 1886 174 1396
Fragments arranged by size:
1886 1396 1183 1035 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2925 4318 4505 1700
Size: 110 1115 1393 187 2869
Fragments arranged by size:
2869 1393 1115 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2801 2626
Size: 175 5499
BstYI r'GATC_y
Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669
Size: 1032 367 385 17 1452 86 12 346
Cuts at: 5669 1972
Size: 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 3826
Fragments arranged by size:
3826 1296 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2635 487
Size: 683 56 1409 3526
Fragments arranged by size:
3526 1409 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2688 2862 3190 3984 4004 2118
Size: 570 174 328 794 20 3788
Fragments arranged by size:
3788 794 570 328 174 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2382 2448
Size: 54 1425 50 275 18 378 174 66
Cuts at: 2448 2838 3121 4010 5639 8
Size: 390 283 889 1629 43
Fragments arranged by size:
1629 1425 889 390 378 283 275 174
66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 3737 4334 5121 5389 1239
Size: 393 887 1218 597 787 268 1524
Fragments arranged by size:
1524 1218 887 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2251 2719 2251
Size: 468 5206
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373
Size: 32 135 32 2743 32 42 32 2626
Fragments arranged by size:
2743 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 3018 3264 669
Size: 988 281 655 425 246 3079
Fragments arranged by size:
3079 988 655 425 281 246
HindIII A'AGCT_T
Cuts at: 748 2464 748
Size: 1716 3958
HpaI GTT'AAC
Cuts at: 2593 3264 2593
Size: 671 5003
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 4788 4972 1305
Size: 744 2739 184 2007
Fragments arranged by size:
2739 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2840 10
Size: 54 1425 343 552 456 2844
Fragments arranged by size:
2844 1425 552 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2016 2240 2624 2654 2745 2756 3632
Size: 648 850 224 384 30 91 11 876
Cuts at: 3632 3991 4150 518
Size: 359 159 2042
Fragments arranged by size:
2042 876 850 648 384 359 224 159
91 30 11
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 3826
Fragments arranged by size:
3826 1296 552
NdeI CA'TA_TG
Cuts at: 387 2325 387
Size: 1938 3736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2825 3032 4577 1132
Size: 97 1596 207 1545 2229
Fragments arranged by size:
2229 1596 1545 207 97
PasI CC'CwG_GG
Cuts at: 1348 2063 1348
Size: 715 4959
PciI A'CATG_T
Cuts at: 2821 4573 2821
Size: 1752 3922
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2265 1108
Size: 1157 4517
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2041 2125 3026 830
Size: 1211 84 901 3478
Fragments arranged by size:
3478 1211 901 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2037 2121 3022 4157 4838 5029 826
Size: 219 992 84 901 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 901 681 219 191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3778 4679 4941 820
Size: 197 926 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2812 3598 5
Size: 47 2760 786 2081
Fragments arranged by size:
2760 2081 786 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2247 2308 2361 3113 513
Size: 552 1182 61 53 752 3074
Fragments arranged by size:
3074 1182 752 552 61 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3701 3718 3871 4056 4501 325
Size: 1255 741 1380 17 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2658 2690 5634 5666 2658
Size: 32 2944 32 2666
Fragments arranged by size:
2944 2666 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2848 2848
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI