Restriction map for
pFN21AE6074
(Circular) (Six-base) MAPSORT of: pFN21AE6074.seq Check: 6595 from: 1 to: 5674
pFN21AE6074 5674 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2108 2526 3017 1937
Size: 171 418 491 4594
Fragments arranged by size:
4594 491 418 171
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2676 3737 5121 5389 1632
Size: 375 669 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 669 375 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2292 3417 4989 2292
Size: 1125 1572 2977
Fragments arranged by size:
2977 1572 1125
ApaLI G'TGCA_C
Cuts at: 2265 2465 3731 4887 2265
Size: 200 1266 1156 3052
Fragments arranged by size:
3052 1266 1156 200
ApoI r'AATT_y
Cuts at: 1493 2036 2304 2616 2810 2934 2983 3209 3361
Size: 543 268 312 194 124 49 226 152
Cuts at: 3361 5470 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 543 312 268 226 194 152
124 49
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 2999 1870
Size: 73 607 449 4545
Fragments arranged by size:
4545 607 449 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2993 1391
Size: 1602 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2682 928
Size: 971 783 3920
Fragments arranged by size:
3920 971 783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2406 2440 3865 3899 1903
Size: 34 469 34 1425 34 3678
Fragments arranged by size:
3678 1425 469 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2269 2469 3735 4891 1790
Size: 479 200 1266 1156 2573
Fragments arranged by size:
2573 1266 1156 479 200
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2349 4334 1239
Size: 818 292 1985 2579
Fragments arranged by size:
2579 1985 818 292
Bpu10I CC'TnA_GC
Cuts at: 2627 2627
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2669 3799 4664 4962 2669
Size: 1130 865 298 3381
Fragments arranged by size:
3381 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 4337 882
Size: 617 768 2070 2219
Fragments arranged by size:
2219 2070 768 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2782 2812 1178
Size: 30 165 30 1379 30 4040
Fragments arranged by size:
4040 1379 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 2122 4877 5373 2122
Size: 2755 496 2423
Fragments arranged by size:
2755 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4684
Fragments arranged by size:
4684 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2269 2469 3735 3820 4891 2269
Size: 200 1266 85 1071 3052
Fragments arranged by size:
3052 1266 1071 200 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2269 2469 2993 3735 3820 4891 1391
Size: 399 479 200 524 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 479 399 200 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 2409 3563 5293 2409
Size: 1154 1730 2790
Fragments arranged by size:
2790 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3029 844
Size: 1664 521 3489
Fragments arranged by size:
3489 1664 521
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4169 4343 65
Size: 1183 1458 1463 174 1396
Fragments arranged by size:
1463 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 2270 3734 4746 2270
Size: 1464 1012 3198
Fragments arranged by size:
3198 1464 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669
Size: 1032 367 385 17 1452 86 12 346
Cuts at: 5669 1972
Size: 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2109 2109
Size: 5674
Bsu36I CC'TnA_GG
Cuts at: 2921 2921
Size: 5674
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2057 2349 2676 3737 4334 5121
Size: 393 375 50 292 327 1061 597 787
Cuts at: 5121 5389 1239
Size: 268 1524
Fragments arranged by size:
1524 1061 787 597 393 375 327 292
268 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2365
Size: 5674
EcoRI G'AATT_C
Cuts at: 2616 2983 3361 2616
Size: 367 378 4929
Fragments arranged by size:
4929 378 367
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2751 2783 4315
Size: 32 135 32 692 32 455 32 1532
Cuts at: 4315 4347 4389 4421 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1532 692 455 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 3018 3264 669
Size: 988 281 274 806 246 3079
Fragments arranged by size:
3079 988 806 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3632 3991 4150 518
Size: 648 1611 855 359 159 2042
Fragments arranged by size:
2042 1611 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2696 3090 3767 4915 5160 1606
Size: 1090 394 677 1148 245 2120
Fragments arranged by size:
2120 1148 1090 677 394 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 2381 387
Size: 1994 3680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2427 3032 4577 1132
Size: 97 1198 605 1545 2229
Fragments arranged by size:
2229 1545 1198 605 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2217 1613
Size: 604 5070
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspXI vC'TCGA_Gb
Cuts at: 2550 2550
Size: 5674
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2696 2696
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2056 3022 4157 4838 5029 826
Size: 219 1011 966 1135 681 191 1471
Fragments arranged by size:
1471 1135 1011 966 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2550 2684 3778 4679 4941 820
Size: 197 926 607 134 1094 901 262 1553
Fragments arranged by size:
1553 1094 926 901 607 262 197 134
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 2950 152
Size: 2798 2876
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2748 3598 5
Size: 47 2696 850 2081
Fragments arranged by size:
2696 2081 850 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 3113 513
Size: 552 939 923 186 3074
Fragments arranged by size:
3074 939 923 552 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3718 3871 4056 4501 325
Size: 1255 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2041 2181 3920
Size: 274 80 33 51 1443 64 140 1739
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1739 1712 1443 274 140 138 80 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2800 2023
Size: 777 4897
XhoI C'TCGA_G
Cuts at: 1943 2550 1943
Size: 607 5067
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 2200 2620 3803 2200
Size: 420 1183 4071
Fragments arranged by size:
4071 1183 420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI