Restriction map for
pFN21AE6068
(Circular) (Six-base) MAPSORT of: pFN21AE6068.seq Check: 5323 from: 1 to: 5668
pFN21AE6068 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2242 2989 2242
Size: 747 4921
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2707 3731 5115 5383 1632
Size: 1075 1024 1384 268 1917
Fragments arranged by size:
1917 1384 1075 1024 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2742 4567 5544 1203
Size: 1539 1825 977 1327
Fragments arranged by size:
1825 1539 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 2554 4397 2554
Size: 1843 3825
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2730 3411 4983 2730
Size: 681 1572 3415
Fragments arranged by size:
3415 1572 681
ApaLI G'TGCA_C
Cuts at: 2230 3725 4881 2230
Size: 1495 1156 3017
Fragments arranged by size:
3017 1495 1156
ApoI r'AATT_y
Cuts at: 1493 2927 2977 3203 3355 5464 1493
Size: 1434 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1434 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2226 2259 2226
Size: 33 5635
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2989 4444 618
Size: 325 217 627 455 747 1455 1842
Fragments arranged by size:
1842 1455 747 627 455 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2987 1391
Size: 754 842 4072
Fragments arranged by size:
4072 842 754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2581 3560 4770 2581
Size: 979 1210 3479
Fragments arranged by size:
3479 1210 979
BclI T'GATC_A
Cuts at: 1548 2757 1548
Size: 1209 4459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 4279 136
Size: 107 122 71 631 605 631 1976 1525
Fragments arranged by size:
1976 1525 631 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2234 3729 4885 1790
Size: 444 1495 1156 2573
Fragments arranged by size:
2573 1495 1156 444
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 4328 1239
Size: 769 2320 2579
Fragments arranged by size:
2579 2320 769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2479 3793 4658 4956 2261
Size: 218 1314 865 298 2973
Fragments arranged by size:
2973 1314 865 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 2474 5662 2474
Size: 3188 2480
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2931 2961 1178
Size: 30 165 30 603 30 895 30 3885
Fragments arranged by size:
3885 895 603 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2483 1186
Size: 994 303 4371
Fragments arranged by size:
4371 994 303
BseYI C'CCAG_C
Cuts at: 4871 5367 4871
Size: 496 5172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2716 1163
Size: 573 423 557 4115
Fragments arranged by size:
4115 573 557 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2234 2355 3729 3814 4885 2234
Size: 121 1374 85 1071 3017
Fragments arranged by size:
3017 1374 1071 121 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2234 2355 2987 3729 3814 4885
Size: 399 355 89 121 632 742 85 1071
Cuts at: 4885 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 632 399 355 121 89
85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3023 844
Size: 1894 285 3489
Fragments arranged by size:
3489 1894 285
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 4163 4337 65
Size: 1183 870 2045 174 1396
Fragments arranged by size:
2045 1396 1183 870 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4312 4499 1700
Size: 110 368 2134 187 2869
Fragments arranged by size:
2869 2134 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2088 2998 3365 3750 3767 5219 5305 5317
Size: 116 910 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 910 385 367 346 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2238 513
Size: 552 1173 3943
Fragments arranged by size:
3943 1173 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 487
Size: 683 56 1190 3739
Fragments arranged by size:
3739 1190 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DraIII CAC_nnn'GTG
Cuts at: 2229 2229
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2562 2841
Size: 54 1425 50 275 18 552 180 279
Cuts at: 2841 3115 4004 5633 8
Size: 274 889 1629 43
Fragments arranged by size:
1629 1425 889 552 279 275 274 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2707 3731 4328 5115 5383 1239
Size: 393 376 699 1024 597 787 268 1524
Fragments arranged by size:
1524 1024 787 699 597 393 376 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2458 2458
Size: 5668
EcoO109I rG'GnC_Cy
Cuts at: 2170 2170
Size: 5668
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
EcoRV GAT'ATC
Cuts at: 2641 2641
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2506 4815 1678
Size: 828 2309 2531
Fragments arranged by size:
2531 2309 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2031 2038 2063 2540
Size: 32 135 32 434 25 7 25 477
Cuts at: 2540 2572 2602 2634 2799 2831 4309 4341 4383
Size: 32 30 32 165 32 1478 32 42
Cuts at: 4383 4415 1373
Size: 32 2626
Fragments arranged by size:
2626 1478 477 434 165 135 42 32
32 32 32 32 32 32 30 25
25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2246 2993 2246
Size: 747 4921
MfeI C'AATT_G
Cuts at: 2880 3267 2880
Size: 387 5281
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2564 2843 10
Size: 54 1425 343 552 180 279 2835
Fragments arranged by size:
2835 1425 552 343 279 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2360 2837 3626 3985 4144 518
Size: 648 1071 123 477 789 359 159 2042
Fragments arranged by size:
2042 1071 789 648 477 359 159 123
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2238 513
Size: 552 1173 3943
Fragments arranged by size:
3943 1173 552
NdeI CA'TA_TG
Cuts at: 387 2325 2553 387
Size: 1938 228 3502
Fragments arranged by size:
3502 1938 228
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2746 3026 4571 1132
Size: 97 1517 280 1545 2229
Fragments arranged by size:
2229 1545 1517 280 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 2742 4567 2742
Size: 1825 3843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 3016 4151 4832 5023 826
Size: 219 1971 1135 681 191 1471
Fragments arranged by size:
1971 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2458 3772 4673 4935 820
Size: 197 926 333 182 1314 901 262 1553
Fragments arranged by size:
1553 1314 926 901 333 262 197 182
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2238 2436 2565 3107 513
Size: 552 1173 198 129 542 3074
Fragments arranged by size:
3074 1173 552 542 198 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2187 3914
Size: 274 80 33 51 1443 135 75 1727
Cuts at: 3914 5626 96
Size: 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 135 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 5628 5660 2732
Size: 32 2864 32 2740
Fragments arranged by size:
2864 2740 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI