Restriction map for
pFN21AE6064
(Circular) (Six-base) MAPSORT of: pFN21AE6064.seq Check: 1320 from: 1 to: 5665
pFN21AE6064 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2118 3008 1937
Size: 181 890 4594
Fragments arranged by size:
4594 890 181
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 2735 4496 2735
Size: 1761 3904
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2756 1166
Size: 1590 4075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2171 1184
Size: 987 4678
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2048 2974 3200 3352 5461 1493
Size: 555 926 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2280 2990 1870
Size: 73 82 255 710 4545
Fragments arranged by size:
4545 710 255 82 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2984 1391
Size: 1593 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2311 3726 4882 1790
Size: 521 1415 1156 2573
Fragments arranged by size:
2573 1415 1156 521
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2791 2950 4325 1239
Size: 1058 494 159 1375 2579
Fragments arranged by size:
2579 1375 1058 494 159
Bpu10I CC'TnA_GC
Cuts at: 2513 2513
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2386 3456 493
Size: 711 1182 1070 2702
Fragments arranged by size:
2702 1182 1070 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2735 4098 4496 4770 4917 5328 2735
Size: 1363 398 274 147 411 3072
Fragments arranged by size:
3072 1363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2076 2134 2449 2681 2801 4868 5364 2076
Size: 58 315 232 120 2067 496 2377
Fragments arranged by size:
2377 2067 496 315 232 120 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2602 1163
Size: 573 866 4226
Fragments arranged by size:
4226 866 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2709 3115 3876 4904 1540
Size: 275 186 708 406 761 1028 2301
Fragments arranged by size:
2301 1028 761 708 406 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
BsmBI CGTCTCn'nnnn_
Cuts at: 2871 2871
Size: 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 2984 3726 3811 4882 1391
Size: 399 521 673 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 521 399 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2091 2114 2277 2321 2802 3020 844
Size: 1247 23 163 44 481 218 3489
Fragments arranged by size:
3489 1247 481 218 163 44 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 3552 1394
Size: 429 703 1026 3507
Fragments arranged by size:
3507 1026 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 4309 4496 1700
Size: 110 925 1574 187 2869
Fragments arranged by size:
2869 1574 925 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 2578 3725 4737 2578
Size: 1147 1012 3506
Fragments arranged by size:
3506 1147 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2480 794
Size: 1686 3979
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2918 2918
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 2031 2367 2643 2757 513
Size: 552 966 336 276 114 3421
Fragments arranged by size:
3421 966 552 336 276 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2662 487
Size: 683 56 1436 3490
Fragments arranged by size:
3490 1436 683 56
BtsI GCAGTG_nn'
Cuts at: 2481 2564 2590 3181 3975 3995 2481
Size: 83 26 591 794 20 4151
Fragments arranged by size:
4151 794 591 83 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2388 2571
Size: 54 1425 50 275 18 375 183 183
Cuts at: 2571 2760 3112 4001 5630 8
Size: 189 352 889 1629 43
Fragments arranged by size:
1629 1425 889 375 352 275 189 183
183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2618 3595 1353
Size: 749 516 977 3423
Fragments arranged by size:
3423 977 749 516
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2230 2498 4252 4626 4772 387
Size: 1064 138 641 268 1754 374 146 1280
Fragments arranged by size:
1754 1280 1064 641 374 268 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2791 2950 3728 4325 5112 5380
Size: 393 665 494 159 778 597 787 268
Cuts at: 5380 1239
Size: 1524
Fragments arranged by size:
1524 787 778 665 597 494 393 268
159
EcoO109I rG'GnC_Cy
Cuts at: 2080 2914 2958 2080
Size: 834 44 4787
Fragments arranged by size:
4787 834 44
EcoRI G'AATT_C
Cuts at: 2048 2974 3352 2048
Size: 926 378 4361
Fragments arranged by size:
4361 926 378
EcoRV GAT'ATC
Cuts at: 2557 2557
Size: 5665
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 2156 4812 1678
Size: 478 2656 2531
Fragments arranged by size:
2656 2531 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 4306 4338 4380
Size: 32 135 32 702 32 2000 32 42
Cuts at: 4380 4412 1373
Size: 32 2626
Fragments arranged by size:
2626 2000 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2207 2390 2573 2762 10
Size: 54 1425 343 375 183 183 189 2913
Fragments arranged by size:
2913 1425 375 343 189 183 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2189 2756 2786 3623 3982 4141
Size: 648 981 42 567 30 837 359 159
Cuts at: 4141 518
Size: 2042
Fragments arranged by size:
2042 981 837 648 567 359 159 42
30
MspA1I CmG'CkG
Cuts at: 1606 2033 3081 3758 4906 5151 1606
Size: 427 1048 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 677 427 245
NcoI C'CATG_G
Cuts at: 513 1065 2367 513
Size: 552 1302 3811
Fragments arranged by size:
3811 1302 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2701 3023 4568 1132
Size: 97 1472 322 1545 2229
Fragments arranged by size:
2229 1545 1472 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PfoI T'CCnGG_A
Cuts at: 2528 2528
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2595 2627 3734 3766 5247 5279 2595
Size: 32 1107 32 1481 32 2981
Fragments arranged by size:
2981 1481 1107 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2958 2080
Size: 878 4787
PshAI GACnn'nnGTC
Cuts at: 1613 2592 1613
Size: 979 4686
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 3017 830
Size: 2187 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SacII CC_GC'GG
Cuts at: 2034 2034
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SanDI GG'GwC_CC
Cuts at: 2958 2958
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2884 3013 4148 4829 5020 826
Size: 219 1839 129 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 2735 1700
Size: 1035 4630
SmaI CCC'GGG
Cuts at: 1872 2027 2992 1872
Size: 155 965 4545
Fragments arranged by size:
4545 965 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2463 1517
Size: 946 4719
StyI C'CwwG_G
Cuts at: 513 1065 2367 3104 513
Size: 552 1302 737 3074
Fragments arranged by size:
3074 1302 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 2695 3692 3709 3862 4047 4492
Size: 1255 876 239 997 17 153 185 445
Cuts at: 4492 325
Size: 1498
Fragments arranged by size:
1498 1255 997 876 445 239 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 3911 5623
Size: 274 80 33 51 1443 216 1718 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1718 1712 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2815 2260
Size: 555 5110
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2025 2990 1870
Size: 155 965 4545
Fragments arranged by size:
4545 965 155
XmnI GAAnn'nnTTC
Cuts at: 2607 3794 2607
Size: 1187 4478
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SapI SfiI SfoI SrfI SwaI