Restriction map for
pFN21AE6063
(Circular) (Six-base) MAPSORT of: pFN21AE6063.seq Check: 3320 from: 1 to: 5665
pFN21AE6063 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2894 1166
Size: 1728 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 2251 3408 4980 2251
Size: 1157 1572 2936
Fragments arranged by size:
2936 1572 1157
ApaLI G'TGCA_C
Cuts at: 2165 3722 4878 2165
Size: 1557 1156 2952
Fragments arranged by size:
2952 1557 1156
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 2990 1870
Size: 73 730 317 4545
Fragments arranged by size:
4545 730 317 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2486 2646 2984 1391
Size: 1095 160 338 4072
Fragments arranged by size:
4072 1095 338 160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2572 3557 4767 2572
Size: 985 1210 3470
Fragments arranged by size:
3470 1210 985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BfrBI ATG'CAT
Cuts at: 2416 2416
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2641 4276 136
Size: 107 122 71 631 605 969 1635 1525
Fragments arranged by size:
1635 1525 969 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2169 3726 4882 1790
Size: 379 1557 1156 2573
Fragments arranged by size:
2573 1557 1156 379
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2441 1056
Size: 1385 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 2771 4325 1239
Size: 777 755 1554 2579
Fragments arranged by size:
2579 1554 777 755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3790 4655 4953 2122
Size: 1668 865 298 2834
Fragments arranged by size:
2834 1668 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 2659 4328 882
Size: 617 656 504 1669 2219
Fragments arranged by size:
2219 1669 656 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3456 493
Size: 711 1536 716 2702
Fragments arranged by size:
2702 1536 716 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2283 2313 1178
Size: 30 165 30 880 30 4530
Fragments arranged by size:
4530 880 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2326 2455 4868 5364 2326
Size: 129 2413 496 2627
Fragments arranged by size:
2627 2413 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2804 3726 3811 4882 2169
Size: 635 922 85 1071 2952
Fragments arranged by size:
2952 1071 922 635 85
BsmI GAATG_Cn'
Cuts at: 2717 3176 3269 2717
Size: 459 93 5113
Fragments arranged by size:
5113 459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2486 2646 2804 2984 3726 3811
Size: 399 379 317 160 158 180 742 85
Cuts at: 3811 4882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 317 180 160
158 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 844
Size: 2176 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 2667 4160 4334 65
Size: 1183 889 530 1493 174 1396
Fragments arranged by size:
1493 1396 1183 889 530 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 2230 3725 4737 2230
Size: 1495 1012 3158
Fragments arranged by size:
3158 1495 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2479 2476
Size: 3 5662
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2882 2882
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3975 3995 3181
Size: 794 20 4851
Fragments arranged by size:
4851 794 20
ClaI AT'CG_AT
Cuts at: 1554 2120 1554
Size: 566 5099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2218 2253 2522 2557 529
Size: 35 1654 35 269 35 3637
Fragments arranged by size:
3637 1654 269 35 35 35
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2610 2662
Size: 54 1425 50 275 18 522 258 52
Cuts at: 2662 3112 4001 5630 8
Size: 450 889 1629 43
Fragments arranged by size:
1629 1425 889 522 450 275 258 54
52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2894 3595 1353
Size: 740 801 701 3423
Fragments arranged by size:
3423 801 740 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2722 4252 4626 4772 387
Size: 1064 138 1133 1530 374 146 1280
Fragments arranged by size:
1530 1280 1133 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2771 3728 4325 5112 5380 1239
Size: 393 384 755 957 597 787 268 1524
Fragments arranged by size:
1524 957 787 755 597 393 384 268
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 2907 4812 1678
Size: 1229 1905 2531
Fragments arranged by size:
2531 1905 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 2521 3264 2521
Size: 743 4922
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 2612 10
Size: 54 1425 343 522 258 3063
Fragments arranged by size:
3063 1425 522 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 2180 2474 2777 2894 3623 3982
Size: 648 994 20 294 303 117 729 359
Cuts at: 3982 4141 518
Size: 159 2042
Fragments arranged by size:
2042 994 729 648 359 303 294 159
117 20
MspA1I CmG'CkG
Cuts at: 1606 2509 3081 3758 4906 5151 1606
Size: 903 572 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 677 572 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 2437 1052
Size: 1385 4280
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NsiI A_TGCA'T
Cuts at: 2418 2418
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 3023 4568 1132
Size: 97 1794 1545 2229
Fragments arranged by size:
2229 1794 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 1108
Size: 1218 4447
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PmlI CAC'GTG
Cuts at: 2740 2740
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2390 2947 3017 830
Size: 1560 557 70 3478
Fragments arranged by size:
3478 1560 557 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2894 2894
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2386 2943 3013 4148 4829 5020 826
Size: 219 1341 557 70 1135 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 557 219 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2675 2992 1872
Size: 803 317 4545
Fragments arranged by size:
4545 803 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2101 3769 4670 4932 820
Size: 197 926 158 1668 901 262 1553
Fragments arranged by size:
1668 1553 926 901 262 197 158
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SrfI GCCC'GGGC
Cuts at: 2675 2675
Size: 5665
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2431 3104 513
Size: 552 1366 673 3074
Fragments arranged by size:
3074 1366 673 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3911 5623
Size: 274 80 33 51 1443 207 1727 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1727 1712 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 5625 5657 2283
Size: 32 3310 32 2291
Fragments arranged by size:
3310 2291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2673 2990 1870
Size: 803 317 4545
Fragments arranged by size:
4545 803 317
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SwaI