Restriction map for
pFN21AE6056
(Circular) (Six-base) MAPSORT of: pFN21AE6056.seq Check: 8882 from: 1 to: 5662
pFN21AE6056 5662 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3725 5109 5377 1632
Size: 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 2367 4493 2367
Size: 2126 3536
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2424 2814 1166
Size: 1258 390 4014
Fragments arranged by size:
4014 1258 390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2421
Size: 32 5630
AlwNI CAG_nnn'CTG
Cuts at: 3405 4977 3405
Size: 1572 4090
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2028 2036 2971 3197 3349 5458 1493
Size: 535 8 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 535 226 152 8
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2987 1870
Size: 73 180 864 4545
Fragments arranged by size:
4545 864 180 73
AvrII C'CTAG_G
Cuts at: 2770 2770
Size: 5662
BamHI G'GATC_C
Cuts at: 2643 2992 3359 2643
Size: 349 367 4946
Fragments arranged by size:
4946 367 349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 2981 1391
Size: 1514 76 4072
Fragments arranged by size:
4072 1514 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 2960 3723 4879 1790
Size: 1170 763 1156 2573
Fragments arranged by size:
2573 1170 1156 763
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2649 2954 3393
Size: 416 53 83 399 24 1232 305 439
Cuts at: 3393 4351 442
Size: 958 1753
Fragments arranged by size:
1753 1232 958 439 416 399 305 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4322 1239
Size: 3083 2579
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2797 3787 4652 4950 2797
Size: 990 865 298 3509
Fragments arranged by size:
3509 990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 2255 5656 2255
Size: 3401 2261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 4095 4493 4767 4914 5325 2367
Size: 1728 398 274 147 411 2704
Fragments arranged by size:
2704 1728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5662
BseYI C'CCAG_C
Cuts at: 2620 4865 5361 2620
Size: 2245 496 2921
Fragments arranged by size:
2921 2245 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2494 1163
Size: 573 642 116 4331
Fragments arranged by size:
4331 642 573 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2464 3723 3808 4879 2124
Size: 340 1259 85 1071 2907
Fragments arranged by size:
2907 1259 1071 340 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2464 2905 2960 2981 3723 3808
Size: 399 334 340 441 55 21 742 85
Cuts at: 3808 4879 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 340 334 85
55 21
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3017 844
Size: 1295 878 3489
Fragments arranged by size:
3489 1295 878
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4306 4493 1700
Size: 110 557 1939 187 2869
Fragments arranged by size:
2869 1939 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 2845 1184
Size: 1661 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2110
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2643 2992 3359 3744 3761 5213 5299 5311
Size: 671 349 367 385 17 1452 86 12
Cuts at: 5311 5657 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 671 385 367 349 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4171
Fragments arranged by size:
4171 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2376 3109
Size: 54 1425 50 275 18 315 231 733
Cuts at: 3109 3998 5627 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 733 315 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3592 1353
Size: 2239 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3725 4322 5109 5377 1239
Size: 393 2093 597 787 268 1524
Fragments arranged by size:
2093 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2630 2630
Size: 5662
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373
Size: 32 135 32 2731 32 42 32 2626
Fragments arranged by size:
2731 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 2584 4776 4960 1305
Size: 915 364 2192 184 2007
Fragments arranged by size:
2192 2007 915 364 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2378 10
Size: 54 1425 343 315 231 3294
Fragments arranged by size:
3294 1425 343 315 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2303 2424 2814 2852 3620 3979
Size: 648 1011 126 121 390 38 768 359
Cuts at: 3979 4138 518
Size: 159 2042
Fragments arranged by size:
2042 1011 768 648 390 359 159 126
121 38
MspA1I CmG'CkG
Cuts at: 1606 3078 3755 4903 5148 1606
Size: 1472 677 1148 245 2120
Fragments arranged by size:
2120 1472 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4171
Fragments arranged by size:
4171 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2684 3020 4565 1132
Size: 97 1455 336 1545 2229
Fragments arranged by size:
2229 1545 1455 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PfoI T'CCnGG_A
Cuts at: 2447 2447
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2630 2630
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5662
SfcI C'TryA_G
Cuts at: 826 1045 2872 3010 4145 4826 5017 826
Size: 219 1827 138 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2812 3766 4667 4929 820
Size: 197 926 869 954 901 262 1553
Fragments arranged by size:
1553 954 926 901 869 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2004 2770 3101 513
Size: 552 939 766 331 3074
Fragments arranged by size:
3074 939 766 552 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3689 3706 3859 4044 4489 325
Size: 1255 1032 1077 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1077 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2706 2835
Size: 274 80 33 51 1443 204 525 129
Cuts at: 2835 3908 5620 96
Size: 1073 1712 138
Fragments arranged by size:
1712 1443 1073 525 274 204 138 129
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2443 2475 5622 5654 2221
Size: 32 190 32 3147 32 2229
Fragments arranged by size:
3147 2229 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3791 2040
Size: 1751 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI