Restriction map for
pFN21AE6049
(Circular) (Six-base) MAPSORT of: pFN21AE6049.seq Check: 6297 from: 1 to: 5659
pFN21AE6049 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2747 2747
Size: 5659
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2595 3469 278
Size: 53 83 186 936 1059 874 2468
Fragments arranged by size:
2468 1059 936 874 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 3722 5106 5374 1632
Size: 962 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1128 962 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 2867 1166
Size: 1701 3958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2651 1184
Size: 1467 4192
AlwNI CAG_nnn'CTG
Cuts at: 2449 3402 4974 2449
Size: 953 1572 3134
Fragments arranged by size:
3134 1572 953
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2704 2968 3194 3346 5455 1493
Size: 1211 264 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 264 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2803 2984 1870
Size: 73 860 181 4545
Fragments arranged by size:
4545 860 181 73
BamHI G'GATC_C
Cuts at: 2313 2989 3356 2313
Size: 676 367 4616
Fragments arranged by size:
4616 676 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 2920 2978 1391
Size: 787 742 58 4072
Fragments arranged by size:
4072 787 742 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BbvCI CC'TCA_GC
Cuts at: 2349 2349
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 4453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2465 4270 136
Size: 107 122 71 631 605 793 1805 1525
Fragments arranged by size:
1805 1525 793 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 2823 3076 2823
Size: 253 5406
Bme1580I G_kGCm'C
Cuts at: 1790 2256 3720 4876 1790
Size: 466 1464 1156 2573
Fragments arranged by size:
2573 1464 1156 466
BmgBI CAC'GTC
Cuts at: 1318 2833 1318
Size: 1515 4144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2148 3390 4348
Size: 416 53 83 399 24 731 1242 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1242 958 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 2364 2647 4319 1239
Size: 1062 63 283 1672 2579
Fragments arranged by size:
2579 1672 1062 283 63
Bpu10I CC'TnA_GC
Cuts at: 2011 2349 2011
Size: 338 5321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2616 3450 493
Size: 711 1412 834 2702
Fragments arranged by size:
2702 1412 834 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2592 3466 3848 4483
Size: 53 83 186 936 1059 874 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1059 936 874 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 1178
Size: 30 165 30 934 30 4470
Fragments arranged by size:
4470 934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2141 2150 2938 1186
Size: 901 54 9 788 3907
Fragments arranged by size:
3907 901 788 54 9
BseYI C'CCAG_C
Cuts at: 2463 4862 5358 2463
Size: 2399 496 2764
Fragments arranged by size:
2764 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3720 3805 4876 2012
Size: 1708 85 1071 2795
Fragments arranged by size:
2795 1708 1071 85
BsmI GAATG_Cn'
Cuts at: 2149 3170 3263 2149
Size: 1021 93 4545
Fragments arranged by size:
4545 1021 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2599 2599
Size: 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2178 2256 2920 2978 3720 3805
Size: 399 222 166 78 664 58 742 85
Cuts at: 3805 4876 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 222 166 85
78 58
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 3014 844
Size: 1903 267 3489
Fragments arranged by size:
3489 1903 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2279 2279
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2227 2313 2989 3356 3741 3758 5210 5296
Size: 255 86 676 367 385 17 1452 86
Cuts at: 5296 5308 5654 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 676 385 367 346 255 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 3802
Fragments arranged by size:
3802 1305 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2417 3175 3969 3989 2417
Size: 758 794 20 4087
Fragments arranged by size:
4087 794 758 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 2733 3106
Size: 54 1425 50 275 18 886 17 373
Cuts at: 3106 3995 5624 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 886 373 275 54 50
43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2774 3589 1353
Size: 1421 815 3423
Fragments arranged by size:
3423 1421 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2364 2594 2647 3722 4319 5106
Size: 393 669 63 230 53 1075 597 787
Cuts at: 5106 5374 1239
Size: 268 1524
Fragments arranged by size:
1524 1075 787 669 597 393 268 230
63 53
EcoRI G'AATT_C
Cuts at: 2704 2968 3346 2704
Size: 264 378 5017
Fragments arranged by size:
5017 378 264
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 10
Size: 54 1425 343 903 2934
Fragments arranged by size:
2934 1425 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2507 2633 2795 2867 3617 3976
Size: 648 1026 315 126 162 72 750 359
Cuts at: 3976 4135 518
Size: 159 2042
Fragments arranged by size:
2042 1026 750 648 359 315 162 159
126 72
MspA1I CmG'CkG
Cuts at: 1606 2714 2822 3075 3752 4900 5145 1606
Size: 1108 108 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 1108 677 253 245 108
NcoI C'CATG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 3802
Fragments arranged by size:
3802 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2090 2800 3017 4562 1132
Size: 97 861 710 217 1545 2229
Fragments arranged by size:
2229 1545 861 710 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4318
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2822 2822
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SapI GCTCTTCn'nnn_
Cuts at: 2774 2774
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 3007 4142 4823 5014 826
Size: 219 1962 1135 681 191 1471
Fragments arranged by size:
1962 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2090 3017 1132
Size: 958 927 3774
Fragments arranged by size:
3774 958 927
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 1517
Size: 1212 4447
StyI C'CwwG_G
Cuts at: 513 1065 2370 2730 3098 513
Size: 552 1305 360 368 3074
Fragments arranged by size:
3074 1305 552 368 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 3686 3703 3856 4041 4486 325
Size: 1255 817 1289 17 153 185 445 1498
Fragments arranged by size:
1498 1289 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3905 5617
Size: 274 80 33 51 1443 219 1709 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2263
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2593 3467 276
Size: 53 83 186 936 1059 874 2468
Fragments arranged by size:
2468 1059 936 874 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI