Restriction map for
pFN21AE6047
(Circular) (Six-base) MAPSORT of: pFN21AE6047.seq Check: 5980 from: 1 to: 5659
pFN21AE6047 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2418 2980 2418
Size: 562 5097
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2761 3722 5106 5374 1632
Size: 1129 961 1384 268 1917
Fragments arranged by size:
1917 1384 1129 961 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 2744 4558 5535 1203
Size: 1541 1814 977 1327
Fragments arranged by size:
1814 1541 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 4039
AlwNI CAG_nnn'CTG
Cuts at: 2054 2200 3402 4974 2054
Size: 146 1202 1572 2739
Fragments arranged by size:
2739 1572 1202 146
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2057 2931 2968 3194 3346 5455 1493
Size: 564 874 37 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 564 226 152 37
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2402 2435 2402
Size: 33 5626
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2418 2980 4435 618
Size: 325 217 627 631 562 1455 1842
Fragments arranged by size:
1842 1455 631 627 562 325 217
BanII G_rGCy'C
Cuts at: 1391 2651 2978 1391
Size: 1260 327 4072
Fragments arranged by size:
4072 1260 327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2455 928
Size: 971 556 4132
Fragments arranged by size:
4132 971 556
BbvCI CC'TCA_GC
Cuts at: 2117 2117
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2163 1548
Size: 615 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 2213 3076 2213
Size: 863 4796
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2042 3390 4348
Size: 416 53 83 399 24 625 1348 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1348 958 625 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2306 4319 1239
Size: 959 108 2013 2579
Fragments arranged by size:
2579 2013 959 108
Bpu10I CC'TnA_GC
Cuts at: 2117 2738 2117
Size: 621 5038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 2168 2330 5653 2168
Size: 162 3323 2174
Fragments arranged by size:
3323 2174 162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3466 3848 4483
Size: 53 83 186 936 488 1445 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1445 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 4717
Fragments arranged by size:
4717 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2210 1186
Size: 868 156 4635
Fragments arranged by size:
4635 868 156
BseYI C'CCAG_C
Cuts at: 2027 2644 4862 5358 2027
Size: 617 2218 496 2328
Fragments arranged by size:
2328 2218 617 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2347 2478 1163
Size: 573 438 173 131 4344
Fragments arranged by size:
4344 573 438 173 131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2744 3720 3805 4876 2244
Size: 500 976 85 1071 3027
Fragments arranged by size:
3027 1071 976 500 85
BsmI GAATG_Cn'
Cuts at: 2485 3170 3263 2485
Size: 685 93 4881
Fragments arranged by size:
4881 685 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2651 2744 2978 3720 3805 4876
Size: 399 454 407 93 234 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 454 407 399 234 93
85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2334 3548 5278 2334
Size: 1214 1730 2715
Fragments arranged by size:
2715 1730 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 3014 844
Size: 1373 797 3489
Fragments arranged by size:
3489 1373 797
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2269 2531 2791 2176
Size: 93 262 260 5044
Fragments arranged by size:
5044 262 260 93
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2139 2352 2832 513
Size: 552 1074 213 480 3340
Fragments arranged by size:
3340 1074 552 480 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2411 2733 3175 3969 3989 2411
Size: 322 442 794 20 4081
Fragments arranged by size:
4081 794 442 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2271 2397
Size: 54 1425 50 275 18 306 135 126
Cuts at: 2397 2448 2814 3106 3995 5624 8
Size: 51 366 292 889 1629 43
Fragments arranged by size:
1629 1425 889 366 306 292 275 135
126 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 3589 1353
Size: 779 1457 3423
Fragments arranged by size:
3423 1457 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2306 2761 3722 4319 5106 5374
Size: 393 566 108 455 961 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 961 787 597 566 455 393 268
108
EcoO109I rG'GnC_Cy
Cuts at: 2266 2734 2827 2266
Size: 468 93 5098
Fragments arranged by size:
5098 468 93
EcoRI G'AATT_C
Cuts at: 2057 2931 2968 3346 2057
Size: 874 37 378 4370
Fragments arranged by size:
4370 874 378 37
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 4300 4332 4374
Size: 32 135 32 707 32 1989 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 1989 707 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3003 3249 669
Size: 988 281 867 198 246 3079
Fragments arranged by size:
3079 988 867 281 246 198
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2422 2984 2422
Size: 562 5097
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2615 4773 4957 1305
Size: 1310 2158 184 2007
Fragments arranged by size:
2158 2007 1310 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2273 2399 2450 2816
Size: 54 1425 343 306 135 126 51 366
Cuts at: 2816 10
Size: 2853
Fragments arranged by size:
2853 1425 366 343 306 135 126 54
51
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2471 2529 3617 3976 4135 518
Size: 648 1203 102 58 1088 359 159 2042
Fragments arranged by size:
2042 1203 1088 648 359 159 102 58
MspA1I CmG'CkG
Cuts at: 1606 2176 3075 3752 4900 5145 1606
Size: 570 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 570 245
NcoI C'CATG_G
Cuts at: 513 1065 2139 2352 2832 513
Size: 552 1074 213 480 3340
Fragments arranged by size:
3340 1074 552 480 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2748 3017 4562 1132
Size: 97 1519 269 1545 2229
Fragments arranged by size:
2229 1545 1519 269 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 2744 4558 2744
Size: 1814 3845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2280 2626 1108
Size: 1172 346 4141
Fragments arranged by size:
4141 1172 346
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2827 2266
Size: 561 5098
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2949 3011 830
Size: 2119 62 3478
Fragments arranged by size:
3478 2119 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2176 2176
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2945 3007 4142 4823 5014 826
Size: 219 1900 62 1135 681 191 1471
Fragments arranged by size:
1900 1471 1135 681 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2183 1517
Size: 666 4993
StyI C'CwwG_G
Cuts at: 513 1065 2139 2262 2352 2832 3098 513
Size: 552 1074 123 90 480 266 3074
Fragments arranged by size:
3074 1074 552 480 266 123 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 2424 2512 3686 3703 3856 4041
Size: 1255 461 383 88 1174 17 153 185
Cuts at: 4041 4486 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1174 461 445 383 185 153
88 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3905 5617
Size: 274 80 33 51 1443 225 1703 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1703 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 5619 5651 2090
Size: 32 3497 32 2098
Fragments arranged by size:
3497 2098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2342 3788 2342
Size: 1446 4213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI