Restriction map for
pFN21AE6046
(Circular) (Six-base) MAPSORT of: pFN21AE6046.seq Check: 8523 from: 1 to: 5659
pFN21AE6046 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2175 2175
Size: 5659
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 3002 1937
Size: 780 285 4594
Fragments arranged by size:
4594 780 285
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3722 5106 5374 1632
Size: 2090 1384 268 1917
Fragments arranged by size:
2090 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 2956 4558 5535 1203
Size: 1753 1602 977 1327
Fragments arranged by size:
1753 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2392 3402 4974 2392
Size: 1010 1572 3077
Fragments arranged by size:
3077 1572 1010
ApaLI G'TGCA_C
Cuts at: 2427 3716 4872 2427
Size: 1289 1156 3214
Fragments arranged by size:
3214 1289 1156
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
AvrII C'CTAG_G
Cuts at: 2240 2240
Size: 5659
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2568 2601 2568
Size: 33 5626
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2055 2978 1391
Size: 664 923 4072
Fragments arranged by size:
4072 923 664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BbvCI CC'TCA_GC
Cuts at: 2550 2550
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2556 2886 1548
Size: 1008 330 4321
Fragments arranged by size:
4321 1008 330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 5654 2337
Size: 3317 2342
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2431 3720 4876 1790
Size: 641 1289 1156 2573
Fragments arranged by size:
2573 1289 1156 641
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 3390 4348
Size: 416 53 83 399 24 640 1333 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1333 958 640 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2960 4319 1239
Size: 1721 1359 2579
Fragments arranged by size:
2579 1721 1359
Bpu10I CC'TnA_GC
Cuts at: 2550 2550
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 1178
Size: 30 165 30 657 30 4747
Fragments arranged by size:
4747 657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2926 1186
Size: 1740 3919
BseYI C'CCAG_C
Cuts at: 2385 4862 5358 2385
Size: 2477 496 2686
Fragments arranged by size:
2686 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2378 2554 1163
Size: 573 417 225 176 4268
Fragments arranged by size:
4268 573 417 225 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 2946 3720 3805 4876 2431
Size: 515 774 85 1071 3214
Fragments arranged by size:
3214 1071 774 515 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 2821
Size: 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2431 2946 2978 3720 3805 4876
Size: 399 265 376 515 32 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 515 399 376 265 85
32
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3014 844
Size: 1331 839 3489
Fragments arranged by size:
3489 1331 839
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 2733 4154 4328 65
Size: 1183 1125 360 1421 174 1396
Fragments arranged by size:
1421 1396 1183 1125 360 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 2620 2743 1184
Size: 1436 123 4100
Fragments arranged by size:
4100 1436 123
BstXI CCAn_nnnn'nTGG
Cuts at: 2324 2324
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2337 2989 3356 3741 3758 5210 5296 5308
Size: 365 652 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 652 385 367 365 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2802 513
Size: 552 1737 3370
Fragments arranged by size:
3370 1737 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2663 3175 3969 3989 2663
Size: 512 794 20 4333
Fragments arranged by size:
4333 794 512 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2813 4666 809
Size: 507 1497 1853 1802
Fragments arranged by size:
1853 1802 1497 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2892 3106 3995
Size: 54 1425 50 275 18 1062 214 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1062 889 275 214 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 2312 3589 1353
Size: 877 82 1277 3423
Fragments arranged by size:
3423 1277 877 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2960 3722 4319 5106 5374 1239
Size: 393 1328 762 597 787 268 1524
Fragments arranged by size:
1524 1328 787 762 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2710 2923 2710
Size: 213 5446
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 4300 4332 4374
Size: 32 135 32 674 32 2022 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 2022 674 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3003 3249 669
Size: 988 281 316 749 246 3079
Fragments arranged by size:
3079 988 749 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 2832 3258 2832
Size: 426 5233
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 4773 4957 1305
Size: 876 2592 184 2007
Fragments arranged by size:
2592 2007 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 10
Size: 54 1425 343 1062 2775
Fragments arranged by size:
2775 1425 1062 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2738 2843 2894 3617 3976 4135
Size: 648 1011 561 105 51 723 359 159
Cuts at: 4135 518
Size: 2042
Fragments arranged by size:
2042 1011 723 648 561 359 159 105
51
MspA1I CmG'CkG
Cuts at: 1606 2500 2554 3075 3752 4900 5145 1606
Size: 894 54 521 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 521 245 54
NcoI C'CATG_G
Cuts at: 513 1065 2802 513
Size: 552 1737 3370
Fragments arranged by size:
3370 1737 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2960 3017 4562 1132
Size: 97 1731 57 1545 2229
Fragments arranged by size:
2229 1731 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 2956 4558 2956
Size: 1602 4057
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 1108
Size: 1402 4257
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2923 2923
Size: 5659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2500 2554 2500
Size: 54 5605
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2118 3007 4142 4823 5014 826
Size: 219 1073 889 1135 681 191 1471
Fragments arranged by size:
1471 1135 1073 889 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2785 1517
Size: 1268 4391
StyI C'CwwG_G
Cuts at: 513 1065 2240 2802 3098 513
Size: 552 1175 562 296 3074
Fragments arranged by size:
3074 1175 562 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3686 3703 3856 4041 4486 325
Size: 1255 860 1246 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1246 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2476 3905 5617
Size: 274 80 33 51 1443 499 1429 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1443 1429 499 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI