Restriction map for
pFN21AE6041
(Circular) (Six-base) MAPSORT of: pFN21AE6041.seq Check: 5400 from: 1 to: 5656
pFN21AE6041 5656 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2222 2999 1937
Size: 285 777 4594
Fragments arranged by size:
4594 777 285
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2702 3719 5103 5371 1632
Size: 653 417 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1017 653 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2079 3399 4971 2079
Size: 1320 1572 2764
Fragments arranged by size:
2764 1572 1320
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2036 2965 3191 3343 5452 1493
Size: 543 929 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2610 4210 160
Size: 872 1578 1600 1606
Fragments arranged by size:
1606 1600 1578 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 5623
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2977 4432 618
Size: 325 217 627 1190 1455 1842
Fragments arranged by size:
1842 1455 1190 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2870 928
Size: 971 971 3714
Fragments arranged by size:
3714 971 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2435 4267 136
Size: 107 122 71 631 605 763 1832 1525
Fragments arranged by size:
1832 1525 763 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 2558 3073 2558
Size: 515 5141
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4600
Fragments arranged by size:
4600 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 2496 4859 5355 2496
Size: 2363 496 2797
Fragments arranged by size:
2797 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4666
Fragments arranged by size:
4666 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 3167 3260 2342
Size: 825 93 4738
Fragments arranged by size:
4738 825 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2975 3717 3802 4873 1391
Size: 399 1185 742 85 1071 2174
Fragments arranged by size:
2174 1185 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2646 3545 5275 2646
Size: 899 1730 3027
Fragments arranged by size:
3027 1730 899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651
Size: 1014 367 385 17 1452 86 12 346
Cuts at: 5651 1972
Size: 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2223 2223
Size: 5656
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3814
Fragments arranged by size:
3814 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2426 2426
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3103 3992
Size: 54 1425 50 275 18 546 727 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 727 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 3586 1353
Size: 1436 797 3423
Fragments arranged by size:
3423 1436 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2702 3719 4316 5103 5371 1239
Size: 393 653 417 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 653 597 417 393 268
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2960 2731
Size: 229 5427
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 2429 2551 4770 4954 1305
Size: 871 253 122 2219 184 2007
Fragments arranged by size:
2219 2007 871 253 184 122
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3288
Fragments arranged by size:
3288 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2594 2774 2780 3614 3973 4132
Size: 648 1011 417 180 6 834 359 159
Cuts at: 4132 518
Size: 2042
Fragments arranged by size:
2042 1011 834 648 417 359 180 159
6
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3814
Fragments arranged by size:
3814 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2134 3014 4559 1132
Size: 97 905 880 1545 2229
Fragments arranged by size:
2229 1545 905 880 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4441
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SapI GCTCTTCn'nnn_
Cuts at: 2789 2789
Size: 5656
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2829 2872 3004 4139 4820 5011 826
Size: 219 1784 43 132 1135 681 191 1471
Fragments arranged by size:
1784 1471 1135 681 219 191 132 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 2257 3580 5
Size: 47 2205 1323 2081
Fragments arranged by size:
2205 2081 1323 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4459
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 2322 2355 3095 513
Size: 552 939 43 275 33 740 3074
Fragments arranged by size:
3074 939 740 552 275 43 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2832 3683 3700 3853 4038 4483 325
Size: 1255 1252 851 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1252 851 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI