Restriction map for
pFN21AE6040
(Circular) (Six-base) MAPSORT of: pFN21AE6040.seq Check: 1936 from: 1 to: 5656
pFN21AE6040 5656 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2576 2576
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2598 2999 1937
Size: 661 401 4594
Fragments arranged by size:
4594 661 401
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 2890 3719 5103 5371 1632
Size: 817 441 829 1384 268 1917
Fragments arranged by size:
1917 1384 829 817 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2011 2965 3191 3343 5452 1493
Size: 518 954 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2790 2981 1870
Size: 73 847 191 4545
Fragments arranged by size:
4545 847 191 73
AvrII C'CTAG_G
Cuts at: 2939 2939
Size: 5656
BamHI G'GATC_C
Cuts at: 2685 2986 3353 2685
Size: 301 367 4988
Fragments arranged by size:
4988 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2977 4432 618
Size: 325 217 627 1190 1455 1842
Fragments arranged by size:
1842 1455 1190 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2918 928
Size: 971 235 784 3666
Fragments arranged by size:
3666 971 784 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2231 4316 1239
Size: 818 174 2085 2579
Fragments arranged by size:
2579 2085 818 174
Bpu10I CC'TnA_GC
Cuts at: 2323 2323
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3447 493
Size: 711 1083 1160 2702
Fragments arranged by size:
2702 1160 1083 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2704 3463 3845 4480
Size: 53 83 186 936 1171 759 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1171 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 2050 2080 2204
Size: 30 165 30 610 30 7 30 124
Cuts at: 2204 2234 2747 2777 1178
Size: 30 513 30 4057
Fragments arranged by size:
4057 610 513 165 124 30 30 30
30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2072 2752 1186
Size: 862 24 680 4090
Fragments arranged by size:
4090 862 680 24
BseYI C'CCAG_C
Cuts at: 2863 4859 5355 2863
Size: 1996 496 3164
Fragments arranged by size:
3164 1996 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2835 3717 3802 4873 2835
Size: 882 85 1071 3618
Fragments arranged by size:
3618 1071 882 85
BsmI GAATG_Cn'
Cuts at: 2134 3167 3260 2134
Size: 1033 93 4530
Fragments arranged by size:
4530 1033 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2835 2975 3717 3802 4873 1391
Size: 399 1045 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 1045 742 399 140 85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2576 3011 844
Size: 1732 435 3489
Fragments arranged by size:
3489 1732 435
BsrBI CCG'CTC
Cuts at: 1394 1823 2732 3543 1394
Size: 429 909 811 3507
Fragments arranged by size:
3507 909 811 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 4151 4325 65
Size: 1183 1419 1484 174 1396
Fragments arranged by size:
1484 1419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 2799 96
Size: 2703 2953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2685 2986 3353 3738 3755 5207 5293 5305
Size: 713 301 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 301 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2681 2879 2681
Size: 198 5458
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2156 487
Size: 683 56 930 3987
Fragments arranged by size:
3987 930 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 2658 2748 3172 3966 3986 2461
Size: 197 90 424 794 20 4131
Fragments arranged by size:
4131 794 424 197 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2416 2451 529
Size: 35 1852 35 3734
Fragments arranged by size:
3734 1852 35 35
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2376 2424
Size: 54 1425 50 275 18 364 182 48
Cuts at: 2424 3103 3992 5621 8
Size: 679 889 1629 43
Fragments arranged by size:
1629 1425 889 679 364 275 182 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 3586 1353
Size: 1424 809 3423
Fragments arranged by size:
3423 1424 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2231 2449 2890 3719 4316 5103
Size: 393 425 174 218 441 829 597 787
Cuts at: 5103 5371 1239
Size: 268 1524
Fragments arranged by size:
1524 829 787 597 441 425 393 268
218 174
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2264
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5656
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 4297 4329 4371
Size: 32 135 32 441 32 2252 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 2252 441 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2480 748
Size: 1732 3924
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 2498 3255 2498
Size: 757 4899
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4770 4954 1305
Size: 798 2667 184 2007
Fragments arranged by size:
2667 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 2378 2426 10
Size: 54 1425 343 364 182 48 3240
Fragments arranged by size:
3240 1425 364 343 182 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 3072 3749 4897 5142 1606
Size: 471 995 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 471 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2077 2077
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 3004 4139 4820 5011 826
Size: 219 1959 1135 681 191 1471
Fragments arranged by size:
1959 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2792 2983 1872
Size: 920 191 4545
Fragments arranged by size:
4545 920 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2287 3447 493
Size: 1794 1160 2702
Fragments arranged by size:
2702 1794 1160
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2903 1517
Size: 1386 4270
StyI C'CwwG_G
Cuts at: 513 1065 2191 2939 3095 513
Size: 552 1126 748 156 3074
Fragments arranged by size:
3074 1126 748 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2307 2330
Size: 274 80 33 51 1443 204 126 23
Cuts at: 2330 2799 3902 5614 96
Size: 469 1103 1712 138
Fragments arranged by size:
1712 1443 1103 469 274 204 138 126
80 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2790 2981 1870
Size: 920 191 4545
Fragments arranged by size:
4545 920 191
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI