Restriction map for
pFN21AE6038
(Circular) (Six-base) MAPSORT of: pFN21AE6038.seq Check: 4466 from: 1 to: 5656
pFN21AE6038 5656 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 2521 2482
Size: 39 5617
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2735 2787 4555 5532 1203
Size: 1532 52 1768 977 1327
Fragments arranged by size:
1768 1532 1327 977 52
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 1184
Size: 1236 4420
AlwNI CAG_nnn'CTG
Cuts at: 2260 2536 2940 3399 4971 2260
Size: 276 404 459 1572 2945
Fragments arranged by size:
2945 1572 459 404 276
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2831 2965 3191 3343 5452 1493
Size: 1338 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1338 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 2682 2981 1870
Size: 73 220 519 299 4545
Fragments arranged by size:
4545 519 299 220 73
BamHI G'GATC_C
Cuts at: 2170 2697 2986 3353 2170
Size: 527 289 367 4473
Fragments arranged by size:
4473 527 367 289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2557 2641 2977 4432 618
Size: 325 217 627 770 84 336 1455 1842
Fragments arranged by size:
1842 1455 770 627 336 325 217 84
BanII G_rGCy'C
Cuts at: 1391 2917 2975 1391
Size: 1526 58 4072
Fragments arranged by size:
4072 1526 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 2317 928
Size: 971 392 26 4267
Fragments arranged by size:
4267 971 392 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2467 2501 3847 3881 1903
Size: 34 530 34 1346 34 3678
Fragments arranged by size:
3678 1346 530 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2568 1548
Size: 1020 4636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2369 4267 136
Size: 107 122 71 631 605 697 1898 1525
Fragments arranged by size:
1898 1525 697 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2646 3717 4873 1790
Size: 856 1071 1156 2573
Fragments arranged by size:
2573 1156 1071 856
BmgBI CAC'GTC
Cuts at: 1318 2736 1318
Size: 1418 4238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2962 4316 1239
Size: 1723 1354 2579
Fragments arranged by size:
2579 1723 1354
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2386 3447 493
Size: 711 1182 1061 2702
Fragments arranged by size:
2702 1182 1061 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2935 2965 1178
Size: 30 165 30 1532 30 3869
Fragments arranged by size:
3869 1532 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2935 1186
Size: 1749 3907
BseYI C'CCAG_C
Cuts at: 2335 4859 5355 2335
Size: 2524 496 2636
Fragments arranged by size:
2636 2524 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 2390 2510 1163
Size: 573 554 100 120 4309
Fragments arranged by size:
4309 573 554 120 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2565 3106 3867 4895 1540
Size: 275 186 564 541 761 1028 2301
Fragments arranged by size:
2301 1028 761 564 541 275 186
BsiHKAI G_wGCw'C
Cuts at: 2434 2694 3717 3802 4873 2434
Size: 260 1023 85 1071 3217
Fragments arranged by size:
3217 1071 1023 260 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2434 2646 2694 2917 2975 3717 3802
Size: 399 644 212 48 223 58 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 223 212 85
58 48
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 2521 2785 3011 844
Size: 1638 39 264 226 3489
Fragments arranged by size:
3489 1638 264 226 39
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2560 4300 4487 1700
Size: 110 750 1740 187 2869
Fragments arranged by size:
2869 1740 750 187 110
BsrGI T'GTAC_A
Cuts at: 96 2193 96
Size: 2097 3559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2447 3716 4728 2447
Size: 1269 1012 3375
Fragments arranged by size:
3375 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2349 794
Size: 1555 4101
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2374 2155
Size: 219 5437
BstYI r'GATC_y
Cuts at: 1972 2170 2697 2937 2986 3353 3738 3755 5207
Size: 198 527 240 49 367 385 17 1452
Cuts at: 5207 5293 5305 5651 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 527 385 367 346 240 198
86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2078 2367 513
Size: 552 1013 289 3802
Fragments arranged by size:
3802 1013 552 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2694 3172 3966 3986 2694
Size: 478 794 20 4364
Fragments arranged by size:
4364 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2349 2349
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2361 2388
Size: 54 1425 50 275 18 297 234 27
Cuts at: 2388 2562 2931 3103 3992 5621 8
Size: 174 369 172 889 1629 43
Fragments arranged by size:
1629 1425 889 369 297 275 234 174
172 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2562 3103 1537
Size: 275 750 541 4090
Fragments arranged by size:
4090 750 541 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2771 2849 3586 1353
Size: 1418 78 737 3423
Fragments arranged by size:
3423 1418 737 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2962 3719 4316 5103 5371 1239
Size: 393 1330 757 597 787 268 1524
Fragments arranged by size:
1524 1330 787 757 597 393 268
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 4297 4329 4371
Size: 32 135 32 664 32 2029 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 2029 664 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 3000 3246 669
Size: 988 281 328 734 246 3079
Fragments arranged by size:
3079 988 734 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 2510 3255 2510
Size: 745 4911
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 2390 2933 10
Size: 54 1425 343 297 261 543 2733
Fragments arranged by size:
2733 1425 543 343 297 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 3614 3973 4132 518
Size: 648 1428 1020 359 159 2042
Fragments arranged by size:
2042 1428 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2394 3072 3749 4897 5142 1606
Size: 788 678 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 678 677 245
NaeI GCC'GGC
Cuts at: 2562 2562
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 2367 513
Size: 552 1302 3802
Fragments arranged by size:
3802 1302 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2560 2560
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2791 3014 4559 1132
Size: 97 1562 223 1545 2229
Fragments arranged by size:
2229 1562 1545 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 2787 4555 2787
Size: 1768 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 2940 1108
Size: 1227 605 3824
Fragments arranged by size:
3824 1227 605
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2111 3725 3757 5238 5270 2079
Size: 32 1614 32 1481 32 2465
Fragments arranged by size:
2465 1614 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2799 3008 830
Size: 1969 209 3478
Fragments arranged by size:
3478 1969 209
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2394 2394
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2795 3004 4139 4820 5011 826
Size: 219 1750 209 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 681 219 209 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2369 2369
Size: 5656
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2165 2684 2983 1872
Size: 293 519 299 4545
Fragments arranged by size:
4545 519 299 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SrfI GCCC'GGGC
Cuts at: 2684 2684
Size: 5656
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2726 1517
Size: 1209 4447
StyI C'CwwG_G
Cuts at: 513 1065 2367 2709 3095 513
Size: 552 1302 342 386 3074
Fragments arranged by size:
3074 1302 552 386 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 3683 3700 3853 4038 4483 325
Size: 1255 471 1632 17 153 185 445 1498
Fragments arranged by size:
1632 1498 1255 471 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 3902 5614
Size: 274 80 33 51 1443 216 1709 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2857 2889 2935 2967 5616 5648 2857
Size: 32 46 32 2649 32 2865
Fragments arranged by size:
2865 2649 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2163 2682 2981 1870
Size: 293 519 299 4545
Fragments arranged by size:
4545 519 299 293
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfoI SwaI XcmI