Restriction map for
pFN21AE6033
(Circular) (Six-base) MAPSORT of: pFN21AE6033.seq Check: 8561 from: 1 to: 5653
pFN21AE6033 5653 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3716 5100 5368 1632
Size: 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2248 2424 3396 4968 2248
Size: 176 972 1572 2933
Fragments arranged by size:
2933 1572 972 176
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2974 4429 618
Size: 325 217 627 758 429 1455 1842
Fragments arranged by size:
1842 1455 758 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 2330 2972 1391
Size: 939 642 4072
Fragments arranged by size:
4072 939 642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BbvCI CC'TCA_GC
Cuts at: 2067 2192 2067
Size: 125 5528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2569 3545 4755 2569
Size: 976 1210 3467
Fragments arranged by size:
3467 1210 976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4264 136
Size: 107 122 71 631 605 685 1907 1525
Fragments arranged by size:
1907 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 2453 3070 2453
Size: 617 5036
Bme1580I G_kGCm'C
Cuts at: 1790 3714 4870 1790
Size: 1924 1156 2573
Fragments arranged by size:
2573 1924 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2930 3384 4342
Size: 416 53 83 399 24 1513 454 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1513 958 454 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2468
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 4313 1239
Size: 907 2167 2579
Fragments arranged by size:
2579 2167 907
Bpu10I CC'TnA_GC
Cuts at: 2067 2192 2067
Size: 125 5528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2134 2655 2926 3778 4643 4941 2134
Size: 521 271 852 865 298 2846
Fragments arranged by size:
2846 865 852 521 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2311 3444 493
Size: 711 1107 1133 2702
Fragments arranged by size:
2702 1133 1107 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2301 4856 5352 2301
Size: 2555 496 2602
Fragments arranged by size:
2602 2555 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 1163
Size: 573 780 4300
Fragments arranged by size:
4300 780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 2556 3714 3799 4870 2486
Size: 70 1158 85 1071 3269
Fragments arranged by size:
3269 1158 1071 85 70
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
BsmBI CGTCTCn'nnnn_
Cuts at: 2436 2736 2436
Size: 300 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2486 2556 2972 3714 3799 4870
Size: 399 540 156 70 416 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 540 416 399 156 85
70
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 2438 2788 4297 4484 1700
Size: 110 575 53 350 1509 187 2869
Fragments arranged by size:
2869 1509 575 350 187 110 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2203 3713 4725 2203
Size: 1510 1012 3131
Fragments arranged by size:
3131 1510 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2251 1184
Size: 1067 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2801 2983 3350 3735 3752 5204 5290 5302
Size: 829 182 367 385 17 1452 86 12
Cuts at: 5302 5648 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 182 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 513
Size: 552 939 312 3850
Fragments arranged by size:
3850 939 552 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2501 487
Size: 683 56 1275 3639
Fragments arranged by size:
3639 1275 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2390 3169 3963 3983 2130
Size: 260 779 794 20 3800
Fragments arranged by size:
3800 794 779 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2215 2250 529
Size: 35 1651 35 3932
Fragments arranged by size:
3932 1651 35 35
DraI TTT'AAA
Cuts at: 2859 2957 2859
Size: 98 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2728 4660 809
Size: 507 1412 1932 1802
Fragments arranged by size:
1932 1802 1412 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 2349 2387
Size: 54 1425 50 275 18 483 36 38
Cuts at: 2387 2436 2607 2790 3100 3989 5618 8
Size: 49 171 183 310 889 1629 43
Fragments arranged by size:
1629 1425 889 483 310 275 183 171
54 50 49 43 38 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 3716 4313 5100 5368 1239
Size: 393 514 1570 597 787 268 1524
Fragments arranged by size:
1570 1524 787 597 514 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2721 2901 2910 2721
Size: 180 9 5464
Fragments arranged by size:
5464 180 9
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 4294 4326 4368
Size: 32 135 32 456 32 2234 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 2234 456 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 2609 10
Size: 54 1425 343 483 294 3054
Fragments arranged by size:
3054 1425 483 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2774 3611 3970 4129 518
Size: 648 1011 597 837 359 159 2042
Fragments arranged by size:
2042 1011 837 648 597 359 159
MspA1I CmG'CkG
Cuts at: 1606 2457 2500 2696 3069 3746 4894 5139 1606
Size: 851 43 196 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 373 245 196 43
NaeI GCC'GGC
Cuts at: 2387 2387
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 2004 2316 513
Size: 552 939 312 3850
Fragments arranged by size:
3850 939 552 312
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2385 2385
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2721 2910 2721
Size: 189 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2500 2696 2500
Size: 196 5457
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SexAI A'CCwGG_T
Cuts at: 2736 2736
Size: 5653
SfcI C'TryA_G
Cuts at: 826 1045 3001 4136 4817 5008 826
Size: 219 1956 1135 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 2670 2905 3757 4658 4920
Size: 197 926 170 557 235 852 901 262
Cuts at: 4920 820
Size: 1553
Fragments arranged by size:
1553 926 901 852 557 262 235 197
170
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 2143 152
Size: 1991 3662
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4456
StyI C'CwwG_G
Cuts at: 513 1065 2004 2316 3092 513
Size: 552 939 312 776 3074
Fragments arranged by size:
3074 939 776 552 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2181 3899
Size: 274 80 33 51 1443 123 81 1718
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 123 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2714 2714
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI