Restriction map for
pFN21AE6031
(Circular) (Six-base) MAPSORT of: pFN21AE6031.seq Check: 1536 from: 1 to: 5653
pFN21AE6031 5653 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2914 2996 1937
Size: 977 82 4594
Fragments arranged by size:
4594 977 82
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 2952 3780 4153 1563
Size: 1389 828 373 3063
Fragments arranged by size:
3063 1389 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3716 5100 5368 1632
Size: 1083 1001 1384 268 1917
Fragments arranged by size:
1917 1384 1083 1001 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2577 2778 2917 4552 5529 1203
Size: 1374 201 139 1635 977 1327
Fragments arranged by size:
1635 1374 1327 977 201 139
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 2921 4382 2921
Size: 1461 4192
AleI CACnn'nnGTG
Cuts at: 1166 2027 2629 1166
Size: 861 602 4190
Fragments arranged by size:
4190 861 602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2162 2292 2693 3396 4968 2162
Size: 130 401 703 1572 2847
Fragments arranged by size:
2847 1572 703 401 130
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2036 2128 2962 3188 3340 5449 1493
Size: 543 92 834 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 543 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2169 2972 1391
Size: 778 803 4072
Fragments arranged by size:
4072 803 778
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2596 928
Size: 971 697 3985
Fragments arranged by size:
3985 971 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BfrBI ATG'CAT
Cuts at: 2216 2216
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2786 3714 4870 1790
Size: 996 928 1156 2573
Fragments arranged by size:
2573 1156 996 928
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2049 3778 4643 4941 2049
Size: 1729 865 298 2761
Fragments arranged by size:
2761 1729 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2343 1186
Size: 1157 4496
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4438
Fragments arranged by size:
4438 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3714 3799 4870 3714
Size: 85 1071 4497
Fragments arranged by size:
4497 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2786 2972 3714 3799 4870 1391
Size: 399 379 617 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 399 379 186 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 2409 3542 5272 2409
Size: 1133 1730 2790
Fragments arranged by size:
2790 1730 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 3008 844
Size: 1926 238 3489
Fragments arranged by size:
3489 1926 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4148 4322 65
Size: 1183 1191 1709 174 1396
Fragments arranged by size:
1709 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 4297 4484 1700
Size: 110 575 1912 187 2869
Fragments arranged by size:
2869 1912 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2730 3713 4725 2730
Size: 983 1012 3658
Fragments arranged by size:
3658 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2359 487
Size: 683 56 1133 3781
Fragments arranged by size:
3781 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DraIII CAC_nnn'GTG
Cuts at: 2833 2833
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618
Size: 54 1425 50 275 18 1270 889 1629
Cuts at: 5618 8
Size: 43
Fragments arranged by size:
1629 1425 1270 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2894 3583 1353
Size: 1541 689 3423
Fragments arranged by size:
3423 1541 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2715 3716 4313 5100 5368 1239
Size: 393 1083 1001 597 787 268 1524
Fragments arranged by size:
1524 1083 1001 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2793 2793
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2246 2278 2280
Size: 32 135 32 475 32 167 32 2
Cuts at: 2280 2312 2428 2460 2541 2573 2751 2783 2803
Size: 32 116 32 81 32 178 32 20
Cuts at: 2803 2835 2907 2939 4294 4326 4368 4400 1373
Size: 32 72 32 1355 32 42 32 2626
Fragments arranged by size:
2626 1355 475 178 167 135 116 81
72 42 32 32 32 32 32 32
32 32 32 32 32 32 20 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2915 2997 3243 669
Size: 988 281 977 82 246 3079
Fragments arranged by size:
3079 988 977 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2572 4767 4951 1305
Size: 1267 2195 184 2007
Fragments arranged by size:
2195 2007 1267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2576 2582 2629 2777 3611 3970
Size: 648 861 549 6 47 148 834 359
Cuts at: 3970 4129 518
Size: 159 2042
Fragments arranged by size:
2042 861 834 648 549 359 159 148
47 6
MspA1I CmG'CkG
Cuts at: 1606 2524 2804 3069 3746 4894 5139 1606
Size: 918 280 265 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 280 265 245
NaeI GCC'GGC
Cuts at: 2387 2387
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2385 2385
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NsiI A_TGCA'T
Cuts at: 2218 2218
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2581 2724 2782 2921 3011 4556 1132
Size: 97 1352 143 58 139 90 1545 2229
Fragments arranged by size:
2229 1545 1352 143 139 97 90 58
PasI CC'CwG_GG
Cuts at: 1348 2162 1348
Size: 814 4839
PciI A'CATG_T
Cuts at: 2577 2778 2917 4552 2577
Size: 201 139 1635 3678
Fragments arranged by size:
3678 1635 201 139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 2942 3223 2942
Size: 281 5372
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2524 2804 2524
Size: 280 5373
SalI G'TCGA_C
Cuts at: 1936 2913 2995 1936
Size: 977 82 4594
Fragments arranged by size:
4594 977 82
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2284 3001 4136 4817 5008 826
Size: 219 1239 717 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 717 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2064 3757 4658 4920 820
Size: 197 926 121 1693 901 262 1553
Fragments arranged by size:
1693 1553 926 901 262 197 121
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2047 2323 3092 513
Size: 552 982 276 769 3074
Fragments arranged by size:
3074 982 769 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 2059 1316
Size: 743 4910
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2479 2248
Size: 231 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI