Restriction map for
pFN21AE6015
(Circular) (Six-base) MAPSORT of: pFN21AE6015.seq Check: 3800 from: 1 to: 5650
pFN21AE6015 5650 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 2843 1166
Size: 1677 3973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2584 1184
Size: 1400 4250
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2017 2620 2912 2959 3185 3337 5446 1493
Size: 524 603 292 47 226 152 2109 1697
Fragments arranged by size:
2109 1697 603 524 292 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 2969 1391
Size: 682 896 4072
Fragments arranged by size:
4072 896 682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 4492
Fragments arranged by size:
4492 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2679 2803 3542 4752 2679
Size: 124 739 1210 3577
Fragments arranged by size:
3577 1210 739 124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 3381 4339
Size: 416 53 83 399 24 898 1066 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1066 958 898 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 4310 1239
Size: 818 2253 2579
Fragments arranged by size:
2579 2253 818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5650
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2106 3711 3796 4867 2073
Size: 33 1605 85 1071 2856
Fragments arranged by size:
2856 1605 1071 85 33
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2106 2969 3711 3796 4867 1391
Size: 399 283 33 863 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 283 85 33
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3005 844
Size: 1888 273 3489
Fragments arranged by size:
3489 1888 273
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2232 4145 4319 65
Size: 1183 984 1913 174 1396
Fragments arranged by size:
1913 1396 1183 984 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 4045
Fragments arranged by size:
4045 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2941 2954 2941
Size: 13 5637
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3097 3986
Size: 54 1425 50 275 18 546 721 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 721 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2518 3580 1353
Size: 737 428 1062 3423
Fragments arranged by size:
3423 1062 737 428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 3713 4310 5097 5365 1239
Size: 393 425 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 787 597 425 393 268
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2994 3240 669
Size: 988 281 220 836 246 3079
Fragments arranged by size:
3079 988 836 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4764 4948 1305
Size: 3459 184 2007
Fragments arranged by size:
3459 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3282
Fragments arranged by size:
3282 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2843 3608 3967 4126 518
Size: 648 1239 438 765 359 159 2042
Fragments arranged by size:
2042 1239 765 648 438 359 159
MspA1I CmG'CkG
Cuts at: 1606 3066 3743 4891 5136 1606
Size: 1460 677 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 4045
Fragments arranged by size:
4045 939 552 114
NdeI CA'TA_TG
Cuts at: 387 2736 387
Size: 2349 3301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2806 1108
Size: 1698 3952
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2614 3002 830
Size: 1784 388 3478
Fragments arranged by size:
3478 1784 388
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SapI GCTCTTCn'nnn_
Cuts at: 2518 2518
Size: 5650
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2466 2610 2872 2998 4133 4814 5005
Size: 219 1421 144 262 126 1135 681 191
Cuts at: 5005 826
Size: 1471
Fragments arranged by size:
1471 1421 1135 681 262 219 191 144
126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 2665 3574 5
Size: 47 2613 909 2081
Fragments arranged by size:
2613 2081 909 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 3089 513
Size: 552 939 114 971 3074
Fragments arranged by size:
3074 971 939 552 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 3677 3694 3847 4032 4477 325
Size: 1255 735 1362 17 153 185 445 1498
Fragments arranged by size:
1498 1362 1255 735 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3896 5608
Size: 274 80 33 51 1443 204 1715 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1715 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI