Restriction map for
pFN21AE6012
(Circular) (Six-base) MAPSORT of: pFN21AE6012.seq Check: 8869 from: 1 to: 5647
pFN21AE6012 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2847 2990 1937
Size: 910 143 4594
Fragments arranged by size:
4594 910 143
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2153 2745 1166
Size: 987 592 4068
Fragments arranged by size:
4068 987 592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 1184
Size: 1362 4285
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2968 4423 618
Size: 325 217 627 234 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2610 2966 1391
Size: 1219 356 4072
Fragments arranged by size:
4072 1219 356
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2138 2138
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3838 3872 1903
Size: 34 50 34 1817 34 3678
Fragments arranged by size:
3678 1817 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2061 3539 4749 2061
Size: 1478 1210 2959
Fragments arranged by size:
2959 1478 1210
BclI T'GATC_A
Cuts at: 1548 2616 1548
Size: 1068 4579
BfrBI ATG'CAT
Cuts at: 2666 2666
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2557 5642 2557
Size: 3085 2562
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2772 3708 4864 1790
Size: 982 936 1156 2573
Fragments arranged by size:
2573 1156 982 936
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 4307 1239
Size: 791 2277 2579
Fragments arranged by size:
2579 2277 791
Bpu10I CC'TnA_GC
Cuts at: 2138 2138
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2906 3772 4637 4935 2699
Size: 207 866 865 298 3411
Fragments arranged by size:
3411 866 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 4310 882
Size: 617 519 2292 2219
Fragments arranged by size:
2292 2219 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 2315 2345 1178
Size: 30 165 30 702 30 180 30 4480
Fragments arranged by size:
4480 702 180 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2008 4850 5346 2008
Size: 2842 496 2309
Fragments arranged by size:
2842 2309 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2769 1163
Size: 573 1033 4041
Fragments arranged by size:
4041 1033 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2418 2815 3708 3793 4864 2418
Size: 397 893 85 1071 3201
Fragments arranged by size:
3201 1071 893 397 85
BsmI GAATG_Cn'
Cuts at: 2058 2142 3158 3251 2058
Size: 84 1016 93 4454
Fragments arranged by size:
4454 1016 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2418 2610 2772 2815 2966 3708 3793
Size: 399 628 192 162 43 151 742 85
Cuts at: 3793 4864 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 628 399 192 162 151
85 43
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2562 2832 3536 5266 2562
Size: 270 704 1730 2943
Fragments arranged by size:
2943 1730 704 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3002 844
Size: 1894 264 3489
Fragments arranged by size:
3489 1894 264
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 4142 4316 65
Size: 1183 1282 1612 174 1396
Fragments arranged by size:
1612 1396 1282 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2132 1184
Size: 948 4699
BstXI CCAn_nnnn'nTGG
Cuts at: 2368 2368
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2557 2904 2977 3344 3729 3746 5198 5284
Size: 585 347 73 367 385 17 1452 86
Cuts at: 5284 5296 5642 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 347 346 86
73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2757 3163 3957 3977 2757
Size: 406 794 20 4427
Fragments arranged by size:
4427 794 406 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 3094 3983
Size: 54 1425 50 275 18 528 736 889
Cuts at: 3983 5612 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 736 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 3577 1353
Size: 1130 1094 3423
Fragments arranged by size:
3423 1130 1094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 3710 4307 5094 5362 1239
Size: 393 398 1680 597 787 268 1524
Fragments arranged by size:
1680 1524 787 597 398 393 268
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 4288 4320 4362
Size: 32 135 32 784 32 1900 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1900 784 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 2991 3237 669
Size: 988 281 655 398 246 3079
Fragments arranged by size:
3079 988 655 398 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 2593 3237 2593
Size: 644 5003
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3297
Fragments arranged by size:
3297 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2732 2745 3605 3964 4123 518
Size: 648 987 579 13 860 359 159 2042
Fragments arranged by size:
2042 987 860 648 579 359 159 13
MspA1I CmG'CkG
Cuts at: 1606 2050 2710 3063 3740 4888 5133 1606
Size: 444 660 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 444 353 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NsiI A_TGCA'T
Cuts at: 2668 2668
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2577 2737 3005 4550 1132
Size: 97 1348 160 268 1545 2229
Fragments arranged by size:
2229 1545 1348 268 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 2729 3716 3748 5229 5261 2697
Size: 32 987 32 1481 32 3083
Fragments arranged by size:
3083 1481 987 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2884 2995 4130 4811 5002 826
Size: 219 1839 111 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 2921 3751 4652 4914 820
Size: 197 926 771 207 830 901 262 1553
Fragments arranged by size:
1553 926 901 830 771 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2079 1517
Size: 562 5085
StyI C'CwwG_G
Cuts at: 513 1065 2898 3086 513
Size: 552 1833 188 3074
Fragments arranged by size:
3074 1833 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 3674 3691 3844 4029 4474 325
Size: 1255 1298 796 17 153 185 445 1498
Fragments arranged by size:
1498 1298 1255 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3893 5605
Size: 274 80 33 51 1443 210 1706 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 210 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 5607 5639 2315
Size: 32 3260 32 2323
Fragments arranged by size:
3260 2323 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI