Restriction map for
pFN21AE6005
(Circular) (Six-base) MAPSORT of: pFN21AE6005.seq Check: 3026 from: 1 to: 5647
pFN21AE6005 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2085 2085
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 2769 3710 5094 5362 1632
Size: 1028 109 941 1384 268 1917
Fragments arranged by size:
1917 1384 1028 941 268 109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2729 4546 5523 1203
Size: 1526 1817 977 1327
Fragments arranged by size:
1817 1526 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2771 1166
Size: 1605 4042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2414 1184
Size: 1230 4417
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2460 2972 1870
Size: 73 517 512 4545
Fragments arranged by size:
4545 517 512 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2171 2204 2171
Size: 33 5614
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 2968 4423 618
Size: 325 217 627 960 221 1455 1842
Fragments arranged by size:
1842 1455 960 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2966 1391
Size: 1575 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2236 2236
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 2303 4258
Size: 107 122 71 631 605 535 96 1955
Cuts at: 4258 136
Size: 1525
Fragments arranged by size:
1955 1525 631 605 535 122 107 96
71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 2107 3064 2107
Size: 957 4690
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2430 2519 3378
Size: 416 53 83 399 24 1013 89 859
Cuts at: 3378 4336 442
Size: 958 1753
Fragments arranged by size:
1753 1013 958 859 416 399 89 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4307 1239
Size: 3068 2579
Bpu10I CC'TnA_GC
Cuts at: 2236 2236
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 2093 5641 2093
Size: 3548 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4480
Fragments arranged by size:
4480 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 1186
Size: 1038 4609
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2122 2384 1163
Size: 573 327 59 262 4426
Fragments arranged by size:
4426 573 327 262 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2966 3708 3793 4864 1391
Size: 399 1176 742 85 1071 2174
Fragments arranged by size:
2174 1176 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2649 3002 844
Size: 1241 564 353 3489
Fragments arranged by size:
3489 1241 564 353
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2221
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2088 2977 3344 3729 3746 5198 5284 5296
Size: 116 889 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 889 385 367 346 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2361 2865 513
Size: 552 1296 504 3295
Fragments arranged by size:
3295 1296 552 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2593 487
Size: 683 56 1367 3541
Fragments arranged by size:
3541 1367 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2355 2396 2536 3163 3957 3977 2240
Size: 115 41 140 627 794 20 3910
Fragments arranged by size:
3910 794 627 140 115 41 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2358 2616
Size: 54 1425 50 275 18 369 159 258
Cuts at: 2616 3094 3983 5612 8
Size: 478 889 1629 43
Fragments arranged by size:
1629 1425 889 478 369 275 258 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2788 2861 3577 1353
Size: 1435 73 716 3423
Fragments arranged by size:
3423 1435 716 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2660 2769 3710 4307 5094 5362 1239
Size: 393 1028 109 941 597 787 268 1524
Fragments arranged by size:
1524 1028 941 787 597 393 268 109
EcoO109I rG'GnC_Cy
Cuts at: 2413 2424 2413
Size: 11 5636
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2060 2616 4761 4945 1305
Size: 755 556 2145 184 2007
Fragments arranged by size:
2145 2007 755 556 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2360 2618 10
Size: 54 1425 343 369 159 258 3039
Fragments arranged by size:
3039 1425 369 343 258 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 2612 2745 2771 3605 3964 4123
Size: 648 1392 54 133 26 834 359 159
Cuts at: 4123 518
Size: 2042
Fragments arranged by size:
2042 1392 834 648 359 159 133 54
26
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2361 2865 513
Size: 552 1296 504 3295
Fragments arranged by size:
3295 1296 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2733 3005 4550 1132
Size: 97 1504 272 1545 2229
Fragments arranged by size:
2229 1545 1504 272 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 2729 4546 2729
Size: 1817 3830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2265 1108
Size: 1157 4490
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2424 2413
Size: 11 5636
PshAI GACnn'nnGTC
Cuts at: 1613 2732 1613
Size: 1119 4528
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SanDI GG'GwC_CC
Cuts at: 2424 2424
Size: 5647
SapI GCTCTTCn'nnn_
Cuts at: 2861 2861
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SexAI A'CCwGG_T
Cuts at: 2220 2220
Size: 5647
SfcI C'TryA_G
Cuts at: 826 1045 2995 4130 4811 5002 826
Size: 219 1950 1135 681 191 1471
Fragments arranged by size:
1950 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2462 2974 1872
Size: 590 512 4545
Fragments arranged by size:
4545 590 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SrfI GCCC'GGGC
Cuts at: 2462 2462
Size: 5647
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2067 1517
Size: 550 5097
StyI C'CwwG_G
Cuts at: 513 1065 2361 2409 2865 3086 513
Size: 552 1296 48 456 221 3074
Fragments arranged by size:
3074 1296 552 456 221 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 3674 3691 3844 4029 4474 325
Size: 1255 1298 796 17 153 185 445 1498
Fragments arranged by size:
1498 1298 1255 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2475 2507 5607 5639 2315
Size: 32 128 32 3100 32 2323
Fragments arranged by size:
3100 2323 128 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 2422 1316
Size: 906 200 4541
Fragments arranged by size:
4541 906 200
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2626 2443
Size: 183 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2460 2972 1870
Size: 590 512 4545
Fragments arranged by size:
4545 590 512
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SfiI SfoI SwaI