Restriction map for
pFN21AE5994
(Circular) (Six-base) MAPSORT of: pFN21AE5994.seq Check: 4769 from: 1 to: 5647
pFN21AE5994 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 2679 2890 3710 5094 5362 1632
Size: 947 100 211 820 1384 268 1917
Fragments arranged by size:
1917 1384 947 820 268 211 100
AflII C'TTAA_G
Cuts at: 820 1017 2878 820
Size: 197 1861 3589
Fragments arranged by size:
3589 1861 197
AflIII A'CryG_T
Cuts at: 1203 2723 4546 5523 1203
Size: 1520 1823 977 1327
Fragments arranged by size:
1823 1520 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4423
AlwNI CAG_nnn'CTG
Cuts at: 2424 3390 4962 2424
Size: 966 1572 3109
Fragments arranged by size:
3109 1572 966
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2036 2956 3182 3334 5443 1493
Size: 543 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2968 4423 618
Size: 325 217 627 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2767 2966 1391
Size: 1376 199 4072
Fragments arranged by size:
4072 1376 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 2820 2824 928
Size: 971 786 135 4 3751
Fragments arranged by size:
3751 971 786 135 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 4258 136
Size: 107 122 71 631 605 586 2000 1525
Fragments arranged by size:
2000 1525 631 605 586 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2296 3378 4336
Size: 416 53 83 399 24 879 1082 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1082 958 879 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 2828 2912 4307 1239
Size: 1210 379 84 1395 2579
Fragments arranged by size:
2579 1395 1210 379 84
Bpu10I CC'TnA_GC
Cuts at: 2192 2794 2939 2192
Size: 602 145 4900
Fragments arranged by size:
4900 602 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 2597 5641 2597
Size: 3044 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2901 3454 3836 4471
Size: 53 83 186 936 1368 553 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1368 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2436 2466 1178
Size: 30 165 30 1033 30 4359
Fragments arranged by size:
4359 1033 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 1163
Size: 573 610 4464
Fragments arranged by size:
4464 610 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2767 2966 3708 3793 4864 1391
Size: 399 977 199 742 85 1071 2174
Fragments arranged by size:
2174 1071 977 742 399 199 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2337 794
Size: 1543 4104
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2244 2977 3344 3729 3746 5198 5284 5296
Size: 272 733 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 733 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2403 513
Size: 552 939 399 3757
Fragments arranged by size:
3757 939 552 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 2390 2578 2739 3163 3957 3977 2358
Size: 32 188 161 424 794 20 4028
Fragments arranged by size:
4028 794 424 188 161 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2259 2287
Size: 54 1425 50 275 18 177 252 28
Cuts at: 2287 2406 2628 3094 3983 5612 8
Size: 119 222 466 889 1629 43
Fragments arranged by size:
1629 1425 889 466 275 252 222 177
119 54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 2579 2679 2828 2890 2912 3710
Size: 393 817 130 100 149 62 22 798
Cuts at: 3710 4307 5094 5362 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 817 798 787 597 393 268 149
130 100 62 22
EcoRI G'AATT_C
Cuts at: 2036 2956 3334 2036
Size: 920 378 4349
Fragments arranged by size:
4349 920 378
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 2641 2673 2754
Size: 32 135 32 864 32 173 32 81
Cuts at: 2754 2786 4288 4320 4362 4394 1373
Size: 32 1502 32 42 32 2626
Fragments arranged by size:
2626 1502 864 173 135 81 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2561 748
Size: 1813 3834
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2261 2408 2630 10
Size: 54 1425 343 177 252 147 222 3027
Fragments arranged by size:
3027 1425 343 252 222 177 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2177 2744 2852 3605 3964 4123
Size: 648 879 132 567 108 753 359 159
Cuts at: 4123 518
Size: 2042
Fragments arranged by size:
2042 879 753 648 567 359 159 132
108
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2727 3005 4550 1132
Size: 97 1498 278 1545 2229
Fragments arranged by size:
2229 1545 1498 278 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 2723 4546 2723
Size: 1823 3824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2573 2999 830
Size: 1743 426 3478
Fragments arranged by size:
3478 1743 426
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2569 2872 2995 4130 4811 5002 826
Size: 219 1524 303 123 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 681 303 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2878 3751 4652 4914 820
Size: 197 926 935 873 901 262 1553
Fragments arranged by size:
1553 935 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 3086 513
Size: 552 939 1082 3074
Fragments arranged by size:
3074 1082 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3893 5605
Size: 274 80 33 51 1443 204 1712 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 2819 1316
Size: 1503 4144
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3776 2040
Size: 1736 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI