Restriction map for
pFN21AE5991
(Circular) (Six-base) MAPSORT of: pFN21AE5991.seq Check: 3177 from: 1 to: 5647
pFN21AE5991 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2057 4546 5523 1203
Size: 854 2489 977 1327
Fragments arranged by size:
2489 1327 977 854
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2027 1166
Size: 861 4786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2905 3390 4962 2905
Size: 485 1572 3590
Fragments arranged by size:
3590 1572 485
ApaLI G'TGCA_C
Cuts at: 2722 3704 4860 2722
Size: 982 1156 3509
Fragments arranged by size:
3509 1156 982
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2823 4201 160
Size: 872 1791 1378 1606
Fragments arranged by size:
1791 1606 1378 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2972 1870
Size: 73 121 908 4545
Fragments arranged by size:
4545 908 121 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2968 4423 618
Size: 325 217 627 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2654 2966 1391
Size: 1263 312 4072
Fragments arranged by size:
4072 1263 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 4489
Fragments arranged by size:
4489 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2304 5642 2304
Size: 3338 2309
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2726 3708 4864 1790
Size: 936 982 1156 2573
Fragments arranged by size:
2573 1156 982 936
BmgBI CAC'GTC
Cuts at: 1318 2727 1318
Size: 1409 4238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2534 4307 1239
Size: 854 441 1773 2579
Fragments arranged by size:
2579 1773 854 441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2721 3438 493
Size: 711 1517 717 2702
Fragments arranged by size:
2702 1517 717 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2926 1186
Size: 1740 3907
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2554 1163
Size: 573 642 176 4256
Fragments arranged by size:
4256 642 573 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2726 3708 3793 4864 2550
Size: 176 982 85 1071 3333
Fragments arranged by size:
3333 1071 982 176 85
BsmI GAATG_Cn'
Cuts at: 2467 3158 3251 2467
Size: 691 93 4863
Fragments arranged by size:
4863 691 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2654 2726 2966 3708 3793 4864
Size: 399 760 104 72 240 742 85 1071
Cuts at: 4864 1391
Size: 2174
Fragments arranged by size:
2174 1071 760 742 399 240 104 85
72
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2598 2790 3536 5266 2598
Size: 192 746 1730 2979
Fragments arranged by size:
2979 1730 746 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2781 4291 4478 1700
Size: 110 971 1510 187 2869
Fragments arranged by size:
2869 1510 971 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2905 794
Size: 2111 3536
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2324 2524 2248
Size: 76 200 5371
Fragments arranged by size:
5371 200 76
BstYI r'GATC_y
Cuts at: 1972 2254 2304 2681 2977 3344 3729 3746 5198
Size: 282 50 377 296 367 385 17 1452
Cuts at: 5198 5284 5296 5642 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 377 367 346 296 282
86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3358
Fragments arranged by size:
3358 939 798 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DraIII CAC_nnn'GTG
Cuts at: 2401 2758 2401
Size: 357 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612
Size: 54 1425 50 275 18 1264 889 1629
Cuts at: 5612 8
Size: 43
Fragments arranged by size:
1629 1425 1264 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2754 3577 1353
Size: 1401 823 3423
Fragments arranged by size:
3423 1401 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2716 4234 4608 4754 387
Size: 1064 138 1127 1518 374 146 1280
Fragments arranged by size:
1518 1280 1127 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2534 3710 4307 5094 5362 1239
Size: 393 461 441 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 787 597 461 441 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2710 2710
Size: 5647
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2873 2905 4288
Size: 32 135 32 674 32 595 32 1383
Cuts at: 4288 4320 4362 4394 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1383 674 595 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 2027 2177 2669 2738 3605 3964
Size: 648 859 2 150 492 69 867 359
Cuts at: 3964 4123 518
Size: 159 2042
Fragments arranged by size:
2042 867 859 648 492 359 159 150
69 2
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NaeI GCC'GGC
Cuts at: 2783 2783
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3358
Fragments arranged by size:
3358 939 798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 2781 2781
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2737 3005 4550 1132
Size: 97 1508 268 1545 2229
Fragments arranged by size:
2229 1545 1508 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4018
PfoI T'CCnGG_A
Cuts at: 2046 2208 2046
Size: 162 5485
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2721 2721
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2910 2999 830
Size: 2080 89 3478
Fragments arranged by size:
3478 2080 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2906 2995 4130 4811 5002 826
Size: 219 1861 89 1135 681 191 1471
Fragments arranged by size:
1861 1471 1135 681 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2433 2802 3086 513
Size: 552 939 429 369 284 3074
Fragments arranged by size:
3074 939 552 429 369 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2416 2440 3674 3691 3844 4029 4474
Size: 1255 836 24 1234 17 153 185 445
Cuts at: 4474 325
Size: 1498
Fragments arranged by size:
1498 1255 1234 836 445 185 153 24
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3893 5605
Size: 274 80 33 51 1443 204 1712 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2324 2248
Size: 76 5571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI