Restriction map for
pFN21AE5983
(Circular) (Six-base) MAPSORT of: pFN21AE5983.seq Check: 2377 from: 1 to: 5644
pFN21AE5983 5644 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2412 4543 5520 1203
Size: 1209 2131 977 1327
Fragments arranged by size:
2131 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2846 1166
Size: 1680 3964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4417
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 2064
Size: 32 5612
AlwNI CAG_nnn'CTG
Cuts at: 2599 3387 4959 2599
Size: 788 1572 3284
Fragments arranged by size:
3284 1572 788
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2447 2974 3341 2447
Size: 527 367 4750
Fragments arranged by size:
4750 527 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2706 2765 2965 4420 618
Size: 325 217 627 919 59 200 1455 1842
Fragments arranged by size:
1842 1455 919 627 325 217 200 59
BanII G_rGCy'C
Cuts at: 1391 2963 1391
Size: 1572 4072
BbeI G_GCGC'C
Cuts at: 2710 2710
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2926 928
Size: 971 1027 3646
Fragments arranged by size:
3646 1027 971
BbvCI CC'TCA_GC
Cuts at: 2195 2195
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3835 3869 1903
Size: 34 50 34 1814 34 3678
Fragments arranged by size:
3678 1814 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2896 3536 4746 2896
Size: 640 1210 3794
Fragments arranged by size:
3794 1210 640
BclI T'GATC_A
Cuts at: 1548 2148 1548
Size: 600 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2793 3705 4861 1790
Size: 1003 912 1156 2573
Fragments arranged by size:
2573 1156 1003 912
BmgBI CAC'GTC
Cuts at: 1318 2487 1318
Size: 1169 4475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2791 4304 1239
Size: 1552 1513 2579
Fragments arranged by size:
2579 1552 1513
Bpu10I CC'TnA_GC
Cuts at: 2195 2195
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2669 3769 4634 4932 2669
Size: 1100 865 298 3381
Fragments arranged by size:
3381 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 2147 5638 2147
Size: 3491 2153
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2707 3451 3833 4468
Size: 53 83 186 936 1174 744 382 635
Cuts at: 4468 275
Size: 1451
Fragments arranged by size:
1451 1174 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2542 1186
Size: 1356 4288
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2492 3705 3790 4861 2492
Size: 1213 85 1071 3275
Fragments arranged by size:
3275 1213 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2492 2793 2963 3705 3790 4861 1391
Size: 399 702 301 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 399 301 170 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 4139 4313 65
Size: 1183 987 1904 174 1396
Fragments arranged by size:
1904 1396 1183 987 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2447 2974 3341 3726 3743 5195 5281 5293
Size: 475 527 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 527 475 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2761 2844 513
Size: 552 1696 83 3313
Fragments arranged by size:
3313 1696 552 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2499 2948 2499
Size: 449 5195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2930 4651 809
Size: 507 1614 1721 1802
Fragments arranged by size:
1802 1721 1614 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2379 3091
Size: 54 1425 50 275 18 273 276 712
Cuts at: 3091 3980 5609 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 712 276 275 273 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2780 3574 1353
Size: 740 687 794 3423
Fragments arranged by size:
3423 794 740 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2791 3707 4304 5091 5359 1239
Size: 393 1159 916 597 787 268 1524
Fragments arranged by size:
1524 1159 916 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2710 4791 1678
Size: 1032 2081 2531
Fragments arranged by size:
2531 2081 1032
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2863 2895 4285 4317 4359
Size: 32 135 32 1291 32 1390 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1390 1291 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 2988 3234 669
Size: 988 281 472 578 246 3079
Fragments arranged by size:
3079 988 578 472 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2706 2706
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2381 10
Size: 54 1425 343 273 276 3273
Fragments arranged by size:
3273 1425 343 276 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2846 3602 3961 4120 518
Size: 648 1680 756 359 159 2042
Fragments arranged by size:
2042 1680 756 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NarI GG'CG_CC
Cuts at: 2707 2707
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2761 513
Size: 552 1696 3396
Fragments arranged by size:
3396 1696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2416 3002 4547 1132
Size: 97 1187 586 1545 2229
Fragments arranged by size:
2229 1545 1187 586 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 2412 4543 2412
Size: 2131 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2875 2992 4127 4808 4999 826
Size: 219 1830 117 1135 681 191 1471
Fragments arranged by size:
1830 1471 1135 681 219 191 117
SfoI GGC'GCC
Cuts at: 2708 2708
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3748 4649 4911 820
Size: 197 926 741 1064 901 262 1553
Fragments arranged by size:
1553 1064 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 2134 3568 5
Size: 47 2082 1434 2081
Fragments arranged by size:
2082 2081 1434 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2475 2761 3083 513
Size: 552 1410 286 322 3074
Fragments arranged by size:
3074 1410 552 322 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2302 3890
Size: 274 80 33 51 1443 207 118 1588
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1588 1443 274 207 138 118 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2471 2503 5604 5636 2471
Size: 32 3101 32 2479
Fragments arranged by size:
3101 2479 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2281 3773 2281
Size: 1492 4152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI