Restriction map for
pFN21AE5975
(Circular) (Six-base) MAPSORT of: pFN21AE5975.seq Check: 7463 from: 1 to: 5641
pFN21AE5975 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 2729
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2425 2984 1937
Size: 488 559 4594
Fragments arranged by size:
4594 559 488
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2644 2677 2644
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2682 2971 3338 2682
Size: 289 367 4985
Fragments arranged by size:
4985 367 289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2324 2759 2926 2962
Size: 325 217 627 481 56 435 167 36
Cuts at: 2962 4417 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 481 435 325 217 167
56 36
BanII G_rGCy'C
Cuts at: 1391 2651 2960 1391
Size: 1260 309 4072
Fragments arranged by size:
4072 1260 309
BbeI G_GCGC'C
Cuts at: 2272 2328 2272
Size: 56 5585
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2672 3533 4743 2672
Size: 861 1210 3570
Fragments arranged by size:
3570 1210 861
BclI T'GATC_A
Cuts at: 1548 2736 1548
Size: 1188 4453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2634 4252 136
Size: 107 122 71 631 605 962 1618 1525
Fragments arranged by size:
1618 1525 962 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2408 3058 2408
Size: 650 4991
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2435 1056
Size: 1379 4262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2150 2282 4301 1239
Size: 815 96 132 2019 2579
Fragments arranged by size:
2579 2019 815 132 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2152 2749 4304 882
Size: 617 653 597 1555 2219
Fragments arranged by size:
2219 1555 653 617 597
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 2325 3448 3830
Size: 53 83 186 936 736 56 1123 382
Cuts at: 3830 4465 275
Size: 635 1451
Fragments arranged by size:
1451 1123 936 736 635 382 186 83
56 53
BsaWI w'CCGG_w
Cuts at: 2679 4074 4472 4746 4893 5304 2679
Size: 1395 398 274 147 411 3016
Fragments arranged by size:
3016 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2716 1186
Size: 1420 110 4111
Fragments arranged by size:
4111 1420 110
BseYI C'CCAG_C
Cuts at: 2435 2470 4844 5340 2435
Size: 35 2374 496 2736
Fragments arranged by size:
2736 2374 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2296 3702 3787 4858 2296
Size: 1406 85 1071 3079
Fragments arranged by size:
3079 1406 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2296 2651 2960 3702 3787 4858 1391
Size: 399 506 355 309 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 506 399 355 309 85
BspEI T'CCGG_A
Cuts at: 2679 5304 2679
Size: 2625 3016
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2729 2996 844
Size: 1271 614 267 3489
Fragments arranged by size:
3489 1271 614 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 4285 4472 1700
Size: 110 455 2020 187 2869
Fragments arranged by size:
2869 2020 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2842 1184
Size: 1658 3983
BstYI r'GATC_y
Cuts at: 1972 2541 2682 2971 3338 3723 3740 5192 5278
Size: 569 141 289 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 569 385 367 346 289 141
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2787 2787
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2022 2115 2328 2352 513
Size: 552 957 93 213 24 3802
Fragments arranged by size:
3802 957 552 213 93 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2399 3157 3951 3971 2399
Size: 758 794 20 4069
Fragments arranged by size:
4069 794 758 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2334 2419 2334
Size: 85 5556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2263 2805
Size: 54 1425 50 275 18 231 202 542
Cuts at: 2805 3088 3977 5606 8
Size: 283 889 1629 43
Fragments arranged by size:
1629 1425 889 542 283 275 231 202
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2321 2756 2834 3571 1353
Size: 734 234 435 78 737 3423
Fragments arranged by size:
3423 737 734 435 234 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2150 2282 3704 4301 5088 5356
Size: 393 422 96 132 1422 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 1422 787 597 422 393 268 132
96
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 2192 4147 2192
Size: 1955 3686
HaeII r_GCGC'y
Cuts at: 1678 2272 2328 4788 1678
Size: 594 56 2460 2531
Fragments arranged by size:
2531 2460 594 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2280 2312 4282 4314 4356
Size: 32 135 32 708 32 1970 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1970 708 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2268 2324 2268
Size: 56 5585
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2138 3599 3958 4117 518
Size: 648 855 117 1461 359 159 2042
Fragments arranged by size:
2042 1461 855 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2560 3057 3734 4882 5127 1606
Size: 954 497 677 1148 245 2120
Fragments arranged by size:
2120 1148 954 677 497 245
NaeI GCC'GGC
Cuts at: 2267 2267
Size: 5641
NarI GG'CG_CC
Cuts at: 2269 2325 2269
Size: 56 5585
NcoI C'CATG_G
Cuts at: 513 1065 2022 2352 513
Size: 552 957 330 3802
Fragments arranged by size:
3802 957 552 330
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2265 2265
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 2431 1052
Size: 1379 4262
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2559 2595 2993 830
Size: 1729 36 398 3478
Fragments arranged by size:
3478 1729 398 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2560 2560
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SapI GCTCTTCn'nnn_
Cuts at: 2087 2087
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2426 2555 2591 2869 2989 4124 4805
Size: 219 1381 129 36 278 120 1135 681
Cuts at: 4805 4996 826
Size: 191 1471
Fragments arranged by size:
1471 1381 1135 681 278 219 191 129
120 36
SfoI GGC'GCC
Cuts at: 2270 2326 2270
Size: 56 5585
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4447
StyI C'CwwG_G
Cuts at: 513 1065 2022 2205 2352 3080 513
Size: 552 957 183 147 728 3074
Fragments arranged by size:
3074 957 728 552 183 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3887 5599
Size: 274 80 33 51 1443 201 1709 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2815 2258
Size: 557 5084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI