Restriction map for
pFN21AE5971
(Circular) (Six-base) MAPSORT of: pFN21AE5971.seq Check: 5188 from: 1 to: 5641
pFN21AE5971 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2175 2546 2732 2175
Size: 371 186 5084
Fragments arranged by size:
5084 371 186
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2397 2962 2397
Size: 565 5076
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3704 5088 5356 1632
Size: 947 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2577 4540 5517 1203
Size: 1374 1963 977 1327
Fragments arranged by size:
1963 1374 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2177 2234 1166
Size: 1011 57 4573
Fragments arranged by size:
4573 1011 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2662 1184
Size: 1478 4163
AlwNI CAG_nnn'CTG
Cuts at: 2706 3384 4956 2706
Size: 678 1572 3391
Fragments arranged by size:
3391 1572 678
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2381 2414 2381
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 2686 2762 2962 4417
Size: 325 217 627 610 289 76 200 1455
Cuts at: 4417 618
Size: 1842
Fragments arranged by size:
1842 1455 627 610 325 289 217 200
76
BanII G_rGCy'C
Cuts at: 1391 2330 2612 2960 1391
Size: 939 282 348 4072
Fragments arranged by size:
4072 939 348 282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 2598 2815 928
Size: 971 380 319 217 3754
Fragments arranged by size:
3754 971 380 319 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 2357 4252
Size: 107 122 71 631 605 469 216 1895
Cuts at: 4252 136
Size: 1525
Fragments arranged by size:
1895 1525 631 605 469 216 122 107
71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2098 3058 2098
Size: 960 4681
Bme1580I G_kGCm'C
Cuts at: 1790 2691 3702 4858 1790
Size: 901 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 901
BmgBI CAC'GTC
Cuts at: 1318 2580 1318
Size: 1262 4379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2920 4301 1239
Size: 854 827 1381 2579
Fragments arranged by size:
2579 1381 854 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 3766 4631 4929 2450
Size: 1316 865 298 3162
Fragments arranged by size:
3162 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2404 2521 3432 493
Size: 711 1200 117 911 2702
Fragments arranged by size:
2702 1200 911 711 117
BsaBI GATnn'nnATC
Cuts at: 2486 5635 2486
Size: 3149 2492
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 1186
Size: 1267 4374
BseYI C'CCAG_C
Cuts at: 2065 4844 5340 2065
Size: 2779 496 2366
Fragments arranged by size:
2779 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4651
Fragments arranged by size:
4651 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2697 3091 3852 4880 1540
Size: 275 186 696 394 761 1028 2301
Fragments arranged by size:
2301 1028 761 696 394 275 186
BsiHKAI G_wGCw'C
Cuts at: 2099 2409 3702 3787 4858 2099
Size: 310 1293 85 1071 2882
Fragments arranged by size:
2882 1293 1071 310 85
BsmI GAATG_Cn'
Cuts at: 2639 3152 3245 2639
Size: 513 93 5035
Fragments arranged by size:
5035 513 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2330 2409 2612 2691 2960 3702
Size: 399 309 231 79 203 79 269 742
Cuts at: 3702 3787 4858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 269 231 203
85 79 79
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2409 3530 5260 2409
Size: 1121 1730 2790
Fragments arranged by size:
2790 1730 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2546 2732 2996 844
Size: 1331 371 186 264 3489
Fragments arranged by size:
3489 1331 371 264 186
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 3528 1394
Size: 429 191 1514 3507
Fragments arranged by size:
3507 1514 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2418 4285 4472 1700
Size: 110 608 1867 187 2869
Fragments arranged by size:
2869 1867 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2113 1184
Size: 929 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2728 2110
Size: 618 5023
BstYI r'GATC_y
Cuts at: 1972 2544 2971 3338 3723 3740 5192 5278 5290
Size: 572 427 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 572 427 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2681 2879 2681
Size: 198 5443
BtgI C'CryG_G
Cuts at: 513 1065 2004 2322 2331 2841 513
Size: 552 939 318 9 510 3313
Fragments arranged by size:
3313 939 552 510 318 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2559 3157 3951 3971 2559
Size: 598 794 20 4229
Fragments arranged by size:
4229 794 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2337 2521 2337
Size: 184 5457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2376 2512
Size: 54 1425 50 275 18 519 27 136
Cuts at: 2512 2670 2694 3088 3977 5606 8
Size: 158 24 394 889 1629 43
Fragments arranged by size:
1629 1425 889 519 394 275 158 136
54 50 43 27 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2694 3088 1537
Size: 275 882 394 4090
Fragments arranged by size:
4090 882 394 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3571 1353
Size: 737 1481 3423
Fragments arranged by size:
3423 1481 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2579 2920 3704 4301 5088 5356
Size: 393 461 486 341 784 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 787 784 597 486 461 393 341
268
EcoO109I rG'GnC_Cy
Cuts at: 2452 2452
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
EcoRV GAT'ATC
Cuts at: 2161 2161
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2401 2966 2401
Size: 565 5076
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 2378 2514 2672 10
Size: 54 1425 343 519 27 136 158 2979
Fragments arranged by size:
2979 1425 519 343 158 136 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2177 2234 2303 2492 2774 2852
Size: 648 978 33 57 69 189 282 78
Cuts at: 2852 3599 3958 4117 518
Size: 747 359 159 2042
Fragments arranged by size:
2042 978 747 648 359 282 189 159
78 69 57 33
MspA1I CmG'CkG
Cuts at: 1606 3057 3734 4882 5127 1606
Size: 1451 677 1148 245 2120
Fragments arranged by size:
2120 1451 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2841 513
Size: 552 939 837 3313
Fragments arranged by size:
3313 939 837 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2571 2999 4544 1132
Size: 97 1342 428 1545 2229
Fragments arranged by size:
2229 1545 1342 428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 1108
Size: 1413 4228
PfoI T'CCnGG_A
Cuts at: 2267 2267
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PmlI CAC'GTG
Cuts at: 2404 2521 2404
Size: 117 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5641
SfcI C'TryA_G
Cuts at: 826 1045 2742 2872 2989 4124 4805 4996 826
Size: 219 1697 130 117 1135 681 191 1471
Fragments arranged by size:
1697 1471 1135 681 219 191 130 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 3745 4646 4908 820
Size: 197 926 522 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 522 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2571 2999 1132
Size: 1439 428 3774
Fragments arranged by size:
3774 1439 428
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2841 3080 513
Size: 552 939 837 239 3074
Fragments arranged by size:
3074 939 837 552 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 3668 3685 3838 4023 4468 325
Size: 1255 992 1096 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1096 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2778 3887
Size: 274 80 33 51 1443 204 597 1109
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 597 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfoI SrfI SwaI XcmI