Restriction map for
pFN21AE5961
(Circular) (Six-base) MAPSORT of: pFN21AE5961.seq Check: 224 from: 1 to: 5641
pFN21AE5961 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 2984 1937
Size: 898 149 4594
Fragments arranged by size:
4594 898 149
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2810 3704 5088 5356 1632
Size: 1178 894 1384 268 1917
Fragments arranged by size:
1917 1384 1178 894 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2723 4540 5517 1203
Size: 1520 1817 977 1327
Fragments arranged by size:
1817 1520 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2824 3384 4956 2824
Size: 560 1572 3509
Fragments arranged by size:
3509 1572 560
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2036 2481 2611 2685 2950 3176 3328 5437
Size: 543 445 130 74 265 226 152 2109
Cuts at: 5437 1493
Size: 1697
Fragments arranged by size:
2109 1697 543 445 265 226 152 130
74
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BbvCI CC'TCA_GC
Cuts at: 2692 2692
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2304 5636 2304
Size: 3332 2309
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 2533 1318
Size: 1215 4426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2048 4301 1239
Size: 802 7 2253 2579
Fragments arranged by size:
2579 2253 802 7
Bpu10I CC'TnA_GC
Cuts at: 2692 2692
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 3432 493
Size: 711 1520 708 2702
Fragments arranged by size:
2702 1520 711 708
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2923 1186
Size: 1737 3904
BseYI C'CCAG_C
Cuts at: 2465 4844 5340 2465
Size: 2379 496 2766
Fragments arranged by size:
2766 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2448 2656 2681 2804 1163
Size: 573 712 208 25 123 4000
Fragments arranged by size:
4000 712 573 208 123 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 2847 3702 3787 4858 2682
Size: 165 855 85 1071 3465
Fragments arranged by size:
3465 1071 855 165 85
BsmI GAATG_Cn'
Cuts at: 2196 2523 3152 3245 2196
Size: 327 629 93 4592
Fragments arranged by size:
4592 629 327 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 2847 2960 3702 3787 4858 1391
Size: 399 892 165 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 892 742 399 165 113 85
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2112 2409 3530 5260 2112
Size: 297 1121 1730 2493
Fragments arranged by size:
2493 1730 1121 297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2996 844
Size: 1295 857 3489
Fragments arranged by size:
3489 1295 857
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 2699 4285 4472 1700
Size: 110 575 314 1586 187 2869
Fragments arranged by size:
2869 1586 575 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2435 794
Size: 1641 4000
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2304 2622 2971 3338 3723 3740 5192 5278
Size: 332 318 349 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 349 346 332 318
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 3157 3951 3971 2835
Size: 322 794 20 4505
Fragments arranged by size:
4505 794 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2426 2426
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606
Size: 54 1425 50 275 18 1258 889 1629
Cuts at: 5606 8
Size: 43
Fragments arranged by size:
1629 1425 1258 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2789 3571 1353
Size: 737 699 782 3423
Fragments arranged by size:
3423 782 737 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2048 2810 3704 4301 5088 5356
Size: 393 409 7 762 894 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 894 787 762 597 409 393 268
7
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2365
Size: 5641
EcoO109I rG'GnC_Cy
Cuts at: 2821 2821
Size: 5641
EcoRI G'AATT_C
Cuts at: 2036 2685 2950 3328 2036
Size: 649 265 378 4349
Fragments arranged by size:
4349 649 378 265
FspI TGC'GCA
Cuts at: 2882 4147 2882
Size: 1265 4376
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2177 2780 3599 3958 4117 518
Size: 648 951 60 603 819 359 159 2042
Fragments arranged by size:
2042 951 819 648 603 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 2228 2696 2788 3057 3734 4882 5127 1606
Size: 622 468 92 269 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 622 468 269 245 92
NaeI GCC'GGC
Cuts at: 2387 2701 2387
Size: 314 5327
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2385 2699 2385
Size: 314 5327
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 2824 1108
Size: 1402 314 3925
Fragments arranged by size:
3925 1402 314
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PmlI CAC'GTG
Cuts at: 2724 2724
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 2609 3211 2609
Size: 602 5039
PstI C_TGCA'G
Cuts at: 830 2304 2993 830
Size: 1474 689 3478
Fragments arranged by size:
3478 1474 689
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2228 2696 2788 2228
Size: 468 92 5081
Fragments arranged by size:
5081 468 92
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SapI GCTCTTCn'nnn_
Cuts at: 2789 2789
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2300 2872 2989 4124 4805 4996 826
Size: 219 1255 572 117 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 681 572 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2323 3080 513
Size: 552 939 319 757 3074
Fragments arranged by size:
3074 939 757 552 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2280 3668 3685 3838 4023 4468
Size: 1255 611 89 1388 17 153 185 445
Cuts at: 4468 325
Size: 1498
Fragments arranged by size:
1498 1388 1255 611 445 185 153 89
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3887 5599 96
Size: 274 80 33 51 1443 1910 1712 138
Fragments arranged by size:
1910 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI