Restriction map for
pFN21AE5959
(Circular) (Six-base) MAPSORT of: pFN21AE5959.seq Check: 1523 from: 1 to: 5641
pFN21AE5959 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2083 3704 5088 5356 1632
Size: 409 42 1621 1384 268 1917
Fragments arranged by size:
1917 1621 1384 409 268 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2356 2461 1184
Size: 1172 105 4364
Fragments arranged by size:
4364 1172 105
AlwNI CAG_nnn'CTG
Cuts at: 2026 3384 4956 2026
Size: 1358 1572 2711
Fragments arranged by size:
2711 1572 1358
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2962 4417 618
Size: 325 217 627 510 665 1455 1842
Fragments arranged by size:
1842 1455 665 627 510 325 217
BanII G_rGCy'C
Cuts at: 1391 2462 2960 1391
Size: 1071 498 4072
Fragments arranged by size:
4072 1071 498
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3832 3866 1903
Size: 34 50 34 1811 34 3678
Fragments arranged by size:
3678 1811 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4252 136
Size: 107 122 71 631 605 685 1895 1525
Fragments arranged by size:
1895 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2064 5636 2064
Size: 3572 2069
BlpI GC'TnA_GC
Cuts at: 2918 3058 2918
Size: 140 5501
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 4645
Fragments arranged by size:
4645 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2152 2420 2719 1186
Size: 966 268 299 4108
Fragments arranged by size:
4108 966 299 268
BseYI C'CCAG_C
Cuts at: 2789 4844 5340 2789
Size: 2055 496 3090
Fragments arranged by size:
3090 2055 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2352 2945 1163
Size: 573 616 593 3859
Fragments arranged by size:
3859 616 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3702 3787 4858 2409
Size: 1293 85 1071 3192
Fragments arranged by size:
3192 1293 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2462 2960 3702 3787 4858 1391
Size: 399 619 53 498 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 498 399 85 53
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2409 3530 5260 2409
Size: 1121 1730 2790
Fragments arranged by size:
2790 1730 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2732 2996 844
Size: 1481 407 264 3489
Fragments arranged by size:
3489 1481 407 264
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2248 2011
Size: 237 5404
BstYI r'GATC_y
Cuts at: 1972 2064 2513 2971 3338 3723 3740 5192 5278
Size: 92 449 458 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 458 449 385 367 346 92
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 3799
Fragments arranged by size:
3799 1290 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 487
Size: 683 56 1247 3655
Fragments arranged by size:
3655 1247 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2077 2262
Size: 54 1425 50 275 18 183 64 185
Cuts at: 2262 3088 3977 5606 8
Size: 826 889 1629 43
Fragments arranged by size:
1629 1425 889 826 275 185 183 64
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3571 1353
Size: 1406 812 3423
Fragments arranged by size:
3423 1406 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2083 3704 4301 5088 5356 1239
Size: 393 409 42 1621 597 787 268 1524
Fragments arranged by size:
1621 1524 787 597 409 393 268 42
EcoO109I rG'GnC_Cy
Cuts at: 2545 2545
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2807 2839 4282 4314 4356
Size: 32 135 32 1235 32 1443 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1443 1235 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 4755 4939 1305
Size: 1379 2071 184 2007
Fragments arranged by size:
2071 2007 1379 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 2264 10
Size: 54 1425 343 183 249 3387
Fragments arranged by size:
3387 1425 343 249 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2492 2648 2741 3599 3958 4117
Size: 648 1137 189 156 93 858 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1137 858 648 359 189 159 156
93
MspA1I CmG'CkG
Cuts at: 1606 2260 3057 3734 4882 5127 1606
Size: 654 797 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 654 245
NcoI C'CATG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 3799
Fragments arranged by size:
3799 1290 552
NdeI CA'TA_TG
Cuts at: 387 2938 387
Size: 2551 3090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2785 2999 4544 1132
Size: 97 1556 214 1545 2229
Fragments arranged by size:
2229 1556 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 2026 1348
Size: 678 4963
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 1108
Size: 918 4723
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2545 2545
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 2192 3211 2192
Size: 1019 4622
PstI C_TGCA'G
Cuts at: 830 2322 2513 2993 830
Size: 1492 191 480 3478
Fragments arranged by size:
3478 1492 480 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2318 2509 2989 4124 4805 4996 826
Size: 219 1273 191 480 1135 681 191 1471
Fragments arranged by size:
1471 1273 1135 681 480 219 191 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 2131 3565 5
Size: 47 2079 1434 2081
Fragments arranged by size:
2081 2079 1434 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4444
StyI C'CwwG_G
Cuts at: 513 1065 2287 2355 3080 513
Size: 552 1222 68 725 3074
Fragments arranged by size:
3074 1222 725 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2018 2272 2280 2315 3668 3685 3838
Size: 1255 438 254 8 35 1353 17 153
Cuts at: 3838 4023 4468 325
Size: 185 445 1498
Fragments arranged by size:
1498 1353 1255 445 438 254 185 153
35 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2307 2601
Size: 274 80 33 51 1443 204 126 294
Cuts at: 2601 3887 5599 96
Size: 1286 1712 138
Fragments arranged by size:
1712 1443 1286 294 274 204 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 5601 5633 2144
Size: 32 3425 32 2152
Fragments arranged by size:
3425 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2027 2023
Size: 4 5637
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI