Restriction map for
pFN21AE5953
(Circular) (Six-base) MAPSORT of: pFN21AE5953.seq Check: 1055 from: 1 to: 5641
pFN21AE5953 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 3704 5088 5356 1632
Size: 927 1145 1384 268 1917
Fragments arranged by size:
1917 1384 1145 927 268
AflII C'TTAA_G
Cuts at: 820 1017 2929 820
Size: 197 1912 3532
Fragments arranged by size:
3532 1912 197
AflIII A'CryG_T
Cuts at: 1203 2728 4540 5517 1203
Size: 1525 1812 977 1327
Fragments arranged by size:
1812 1525 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2228 1166
Size: 1062 4579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2496 1184
Size: 1312 4329
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 2508 3698 4854 2508
Size: 1190 1156 3295
Fragments arranged by size:
3295 1190 1156
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
AvrII C'CTAG_G
Cuts at: 2041 2041
Size: 5641
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2469 2899 2960 1391
Size: 677 401 430 61 4072
Fragments arranged by size:
4072 677 430 401 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2572 928
Size: 971 673 3997
Fragments arranged by size:
3997 971 673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 5636 2298
Size: 3338 2303
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2512 3702 4858 1790
Size: 445 277 1190 1156 2573
Fragments arranged by size:
2573 1190 1156 445 277
BmgBI CAC'GTC
Cuts at: 1318 2209 1318
Size: 891 4750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
Bpu10I CC'TnA_GC
Cuts at: 2186 2489 2186
Size: 303 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2660 2773 4304 882
Size: 617 1161 113 1531 2219
Fragments arranged by size:
2219 1531 1161 617 113
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2258 4074 4472 4746 4893 5304 2258
Size: 1816 398 274 147 411 2595
Fragments arranged by size:
2595 1816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2475 2660 1163
Size: 573 579 160 185 4144
Fragments arranged by size:
4144 579 573 185 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2512 3702 3787 4858 2512
Size: 1190 85 1071 3295
Fragments arranged by size:
3295 1190 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2235 2469 2512 2899 2960 3702
Size: 399 278 167 234 43 387 61 742
Cuts at: 3702 3787 4858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 278 234 167
85 61 43
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2082 2130 2403 3530 5260 2082
Size: 48 273 1127 1730 2463
Fragments arranged by size:
2463 1730 1127 273 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 2996 844
Size: 1882 270 3489
Fragments arranged by size:
3489 1882 270
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 4285 4472 1700
Size: 110 347 2128 187 2869
Fragments arranged by size:
2869 2128 347 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2513 3701 4713 2513
Size: 1188 1012 3441
Fragments arranged by size:
3441 1188 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 2583 4445 2583
Size: 1862 3779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2298 2971 3338 3723 3740 5192 5278 5290
Size: 326 673 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 673 385 367 346 326 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2423 2423
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3805
Fragments arranged by size:
3805 939 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 2520 1554
Size: 966 4675
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2576 4648 809
Size: 507 1260 2072 1802
Fragments arranged by size:
2072 1802 1260 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606
Size: 54 1425 50 275 18 1258 889 1629
Cuts at: 5606 8
Size: 43
Fragments arranged by size:
1629 1425 1258 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2728 3571 1353
Size: 731 644 843 3423
Fragments arranged by size:
3423 843 731 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 3704 4301 5088 5356 1239
Size: 393 927 1145 597 787 268 1524
Fragments arranged by size:
1524 1145 927 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2539 2539
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
EcoRV GAT'ATC
Cuts at: 2393 2393
Size: 5641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2207 2228 2402 3599 3958 4117
Size: 648 1005 36 21 174 1197 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1197 1005 648 359 174 159 36
21
MspA1I CmG'CkG
Cuts at: 1606 2416 2459 3057 3734 4882 5127 1606
Size: 810 43 598 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 598 245 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3805
Fragments arranged by size:
3805 939 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2683 2732 2999 4544 1132
Size: 97 1454 49 267 1545 2229
Fragments arranged by size:
2229 1545 1454 267 97 49
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4672
PciI A'CATG_T
Cuts at: 2728 4540 2728
Size: 1812 3829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2475 2993 830
Size: 1645 518 3478
Fragments arranged by size:
3478 1645 518
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2416 2459 2416
Size: 43 5598
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SapI GCTCTTCn'nnn_
Cuts at: 2728 2728
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2475 2993 2475
Size: 518 5123
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2471 2688 2866 2989 4124 4805 4996
Size: 219 1426 217 178 123 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1426 1135 681 219 217 191 178
123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2929 3745 4646 4908 820
Size: 197 926 986 816 901 262 1553
Fragments arranged by size:
1553 986 926 901 816 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4450
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2349 3080 513
Size: 552 939 37 308 731 3074
Fragments arranged by size:
3074 939 731 552 308 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2309 2397 2441 3668 3685 3838 4023
Size: 1255 729 88 44 1227 17 153 185
Cuts at: 4023 4468 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1227 729 445 185 153 88
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3887 5599
Size: 274 80 33 51 1443 198 1712 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 4523
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI