Restriction map for
pFN21AE5948
(Circular) (Six-base) MAPSORT of: pFN21AE5948.seq Check: 3579 from: 1 to: 5641
pFN21AE5948 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2508 2508
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 3704 5088 5356 1632
Size: 1034 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1038 1034 268
AflII C'TTAA_G
Cuts at: 820 1017 2782 820
Size: 197 1765 3679
Fragments arranged by size:
3679 1765 197
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 2260 4370 2260
Size: 2110 3531
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2080 3384 4956 2080
Size: 1304 1572 2765
Fragments arranged by size:
2765 1572 1304
ApaI G_GGCC'C
Cuts at: 2167 2167
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2712 2745 2712
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2962 4417 618
Size: 325 217 627 336 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 336 325 217
BanII G_rGCy'C
Cuts at: 1391 2167 2960 1391
Size: 776 793 4072
Fragments arranged by size:
4072 793 776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 928
Size: 971 380 4290
Fragments arranged by size:
4290 971 380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2842 3533 4743 2842
Size: 691 1210 3740
Fragments arranged by size:
3740 1210 691
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2167 3702 4858 1790
Size: 336 41 1535 1156 2573
Fragments arranged by size:
2573 1535 1156 336 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2708 3372 4330
Size: 416 53 83 399 24 1291 664 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1291 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2791 2899 3766 4631 4929 2543
Size: 248 108 867 865 298 3255
Fragments arranged by size:
3255 867 865 298 248 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 2597 5635 2597
Size: 3038 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2794 1186
Size: 907 701 4033
Fragments arranged by size:
4033 907 701
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2167 2960 3702 3787 4858 1391
Size: 399 336 41 793 742 85 1071 2174
Fragments arranged by size:
2174 1071 793 742 399 336 85 41
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2508 2996 844
Size: 1421 243 488 3489
Fragments arranged by size:
3489 1421 488 243
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3528 1394
Size: 429 188 1517 3507
Fragments arranged by size:
3507 1517 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2544 4136 4310 65
Size: 1183 1296 1592 174 1396
Fragments arranged by size:
1592 1396 1296 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 2612 96
Size: 2516 3125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4355
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2248 2155
Size: 93 5548
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3157 3951 3971 2390
Size: 767 794 20 4060
Fragments arranged by size:
4060 794 767 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3088 3977
Size: 54 1425 50 275 18 234 1024 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1024 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 3571 1353
Size: 971 1247 3423
Fragments arranged by size:
3423 1247 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2666 3704 4301 5088 5356 1239
Size: 393 1034 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 1034 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2163 2164 2163
Size: 1 5640
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2246 2278 2882
Size: 32 135 32 453 32 189 32 604
Cuts at: 2882 2914 4282 4314 4356 4388 1373
Size: 32 1368 32 42 32 2626
Fragments arranged by size:
2626 1368 604 453 189 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 2985 3231 669
Size: 988 281 316 731 246 3079
Fragments arranged by size:
3079 988 731 316 281 246
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 3677
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 4755 4939 1305
Size: 1515 1935 184 2007
Fragments arranged by size:
2007 1935 1515 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 10
Size: 54 1425 343 234 3585
Fragments arranged by size:
3585 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2603 3599 3958 4117 518
Size: 648 1137 300 996 359 159 2042
Fragments arranged by size:
2042 1137 996 648 359 300 159
MspA1I CmG'CkG
Cuts at: 1606 2080 3057 3734 4882 5127 1606
Size: 474 977 677 1148 245 2120
Fragments arranged by size:
2120 1148 977 677 474 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 2846 3211 2846
Size: 365 5276
PspOMI G'GGCC_C
Cuts at: 2163 2163
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2076 2993 830
Size: 1246 917 3478
Fragments arranged by size:
3478 1246 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2080 2080
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2072 2989 4124 4805 4996 826
Size: 219 1027 917 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 917 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2782 2806 2878 3745 4646
Size: 197 926 615 224 24 72 867 901
Cuts at: 4646 4908 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 867 615 262 224 197
72 24
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 2434 152
Size: 2282 3359
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2788 1517
Size: 1271 4370
StyI C'CwwG_G
Cuts at: 513 1065 2004 2323 2487 3080 513
Size: 552 939 319 164 593 3074
Fragments arranged by size:
3074 939 593 552 319 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2612 3887
Size: 274 80 33 51 1443 204 431 1275
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1275 431 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 5601 5633 2406
Size: 32 3163 32 2414
Fragments arranged by size:
3163 2414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2284 2152
Size: 132 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2929 3770 2929
Size: 841 4800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI