Restriction map for
pFN21AE5943
(Circular) (Six-base) MAPSORT of: pFN21AE5943.seq Check: 1080 from: 1 to: 5641
pFN21AE5943 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 2367 4472 2367
Size: 2105 3536
AhdI GACnn_n'nnGTC
Cuts at: 2774 4370 2774
Size: 1596 4045
AleI CACnn'nnGTG
Cuts at: 1166 2303 2739 1166
Size: 1137 436 4068
Fragments arranged by size:
4068 1137 436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2519 3384 4956 2519
Size: 865 1572 3204
Fragments arranged by size:
3204 1572 865
ApaI G_GGCC'C
Cuts at: 2905 2905
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2036 2950 3176 3328 5437 1493
Size: 543 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2643 2971 3338 2643
Size: 328 367 4946
Fragments arranged by size:
4946 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2292 2962 4417 618
Size: 325 217 627 505 670 1455 1842
Fragments arranged by size:
1842 1455 670 627 505 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 2960 1391
Size: 1514 55 4072
Fragments arranged by size:
4072 1514 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2329 3533 4743 2329
Size: 1204 1210 3227
Fragments arranged by size:
3227 1210 1204
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 2427 4252
Size: 107 122 71 631 605 685 70 1825
Cuts at: 4252 136
Size: 1525
Fragments arranged by size:
1825 1525 685 631 605 122 107 71
70
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2905 3702 4858 1790
Size: 1115 797 1156 2573
Fragments arranged by size:
2573 1156 1115 797
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
Bpu10I CC'TnA_GC
Cuts at: 2550 2918 2550
Size: 368 5273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3766 4631 4929 2543
Size: 1223 865 298 3255
Fragments arranged by size:
3255 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2752 4304 882
Size: 617 1253 1552 2219
Fragments arranged by size:
2219 1552 1253 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 4074 4472 4746 4893 5304 2367
Size: 1707 398 274 147 411 2704
Fragments arranged by size:
2704 1707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2573 2662 2906 1186
Size: 847 540 89 244 3921
Fragments arranged by size:
3921 847 540 244 89
BseYI C'CCAG_C
Cuts at: 2122 2620 2639 4844 5340 2122
Size: 498 19 2205 496 2423
Fragments arranged by size:
2423 2205 498 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4426
Fragments arranged by size:
4426 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2905 2960 3702 3787 4858 1391
Size: 399 1115 55 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 742 399 85 55
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2409 3530 5260 2409
Size: 1121 1730 2790
Fragments arranged by size:
2790 1730 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2996 844
Size: 1295 857 3489
Fragments arranged by size:
3489 1295 857
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 2545 4285 4472 1700
Size: 110 557 178 1740 187 2869
Fragments arranged by size:
2869 1740 557 187 178 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2845 1184
Size: 1661 3980
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2110
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2643 2729 2971 3338 3723 3740 5192 5278
Size: 671 86 242 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 671 385 367 346 242 86
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 2841 513
Size: 552 939 606 231 3313
Fragments arranged by size:
3313 939 606 552 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 2615 3157 3951 3971 2390
Size: 225 542 794 20 4060
Fragments arranged by size:
4060 794 542 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2349 2376
Size: 54 1425 50 275 18 315 204 27
Cuts at: 2376 2607 3088 3977 5606 8
Size: 231 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 315 275 231 204
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2654 3571 1353
Size: 1136 165 917 3423
Fragments arranged by size:
3423 1136 917 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3704 4301 5088 5356 1239
Size: 393 2072 597 787 268 1524
Fragments arranged by size:
2072 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 5641
EcoO109I rG'GnC_Cy
Cuts at: 2554 2901 2902 2554
Size: 347 1 5293
Fragments arranged by size:
5293 347 1
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2721 2753 2760 2792 4282
Size: 32 135 32 1149 32 7 32 1490
Cuts at: 4282 4314 4356 4388 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1490 1149 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2502 2985 3231 669
Size: 988 281 274 290 483 246 3079
Fragments arranged by size:
3079 988 483 290 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 4755 4939 1305
Size: 915 2535 184 2007
Fragments arranged by size:
2535 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2609 10
Size: 54 1425 343 546 231 3042
Fragments arranged by size:
3042 1425 546 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2303 2739 2852 3599 3958 4117
Size: 648 1011 126 436 113 747 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1011 747 648 436 359 159 126
113
MspA1I CmG'CkG
Cuts at: 1606 3057 3734 4882 5127 1606
Size: 1451 677 1148 245 2120
Fragments arranged by size:
2120 1451 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2841 513
Size: 552 939 837 3313
Fragments arranged by size:
3313 939 837 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 2934 1613
Size: 1321 4320
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2901 2901
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2076 2993 830
Size: 1246 917 3478
Fragments arranged by size:
3478 1246 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5641
SfcI C'TryA_G
Cuts at: 826 1045 2072 2634 2872 2989 4124 4805 4996
Size: 219 1027 562 238 117 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 681 562 238 219 191
117
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3745 4646 4908 820
Size: 197 926 615 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2697 2841 3080 513
Size: 552 939 693 144 239 3074
Fragments arranged by size:
3074 939 693 552 239 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2403 2612 3668 3685 3838 4023 4468
Size: 1255 823 209 1056 17 153 185 445
Cuts at: 4468 325
Size: 1498
Fragments arranged by size:
1498 1255 1056 823 445 209 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2706 3887
Size: 274 80 33 51 1443 204 525 1181
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1181 525 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2509 2541 5601 5633 2509
Size: 32 3060 32 2517
Fragments arranged by size:
3060 2517 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2864 3770 2864
Size: 906 4735
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfoI SrfI SwaI