Restriction map for
pFN21AE5942
(Circular) (Six-base) MAPSORT of: pFN21AE5942.seq Check: 2435 from: 1 to: 5641
pFN21AE5942 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2837 2984 1937
Size: 274 626 147 4594
Fragments arranged by size:
4594 626 274 147
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2481 2892 4540 5517 1203
Size: 1278 411 1648 977 1327
Fragments arranged by size:
1648 1327 1278 977 411
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2036 2343 2523 2685 2950 3176 3328 5437
Size: 543 307 180 162 265 226 152 2109
Cuts at: 5437 1493
Size: 1697
Fragments arranged by size:
2109 1697 543 307 265 226 180 162
152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2062 2971 3338 2062
Size: 909 367 4365
Fragments arranged by size:
4365 909 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2919 3533 4743 2919
Size: 614 1210 3817
Fragments arranged by size:
3817 1210 614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 2791 4301 1239
Size: 777 775 1510 2579
Fragments arranged by size:
2579 1510 777 775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3766 4631 4929 2543
Size: 1223 865 298 3255
Fragments arranged by size:
3255 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2065 4074 4472 4746 4893 5304 2065
Size: 2009 398 274 147 411 2402
Fragments arranged by size:
2402 2009 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 4645
Fragments arranged by size:
4645 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2662 2794 1186
Size: 994 482 132 4033
Fragments arranged by size:
4033 994 482 132
BseYI C'CCAG_C
Cuts at: 2122 4844 5340 2122
Size: 2722 496 2423
Fragments arranged by size:
2722 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 1163
Size: 573 726 4342
Fragments arranged by size:
4342 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2960 3702 3787 4858 1391
Size: 399 1170 742 85 1071 2174
Fragments arranged by size:
2174 1170 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2065 5304 2065
Size: 3239 2402
BspHI T'CATG_A
Cuts at: 2352 2409 2679 3530 5260 2352
Size: 57 270 851 1730 2733
Fragments arranged by size:
2733 1730 851 270 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2996 844
Size: 1295 857 3489
Fragments arranged by size:
3489 1295 857
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2608 2845 1184
Size: 1424 237 3980
Fragments arranged by size:
3980 1424 237
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2122 2248 2110
Size: 12 126 5503
Fragments arranged by size:
5503 126 12
BstYI r'GATC_y
Cuts at: 1972 2062 2971 3338 3723 3740 5192 5278 5290
Size: 90 909 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 909 385 367 346 90 86
17 12
BstZ17I GTA'TAC
Cuts at: 2838 2838
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2841 513
Size: 552 1776 3313
Fragments arranged by size:
3313 1776 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 2368 1554
Size: 814 4827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2407 2442 529
Size: 35 1843 35 3728
Fragments arranged by size:
3728 1843 35 35
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2145 2376
Size: 54 1425 50 275 18 282 33 231
Cuts at: 2376 3088 3977 5606 8
Size: 712 889 1629 43
Fragments arranged by size:
1629 1425 889 712 282 275 231 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3571 1353
Size: 737 1481 3423
Fragments arranged by size:
3423 1481 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2791 3704 4301 5088 5356 1239
Size: 393 384 775 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 775 597 393 384 268
EcoRI G'AATT_C
Cuts at: 2036 2343 2950 3328 2036
Size: 307 607 378 4349
Fragments arranged by size:
4349 607 378 307
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 2212 2985 3231 669
Size: 988 281 214 60 773 246 3079
Fragments arranged by size:
3079 988 773 281 246 214 60
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 3677
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2308 4755 4939 1305
Size: 1003 2447 184 2007
Fragments arranged by size:
2447 2007 1003 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2378 10
Size: 54 1425 343 282 264 3273
Fragments arranged by size:
3273 1425 343 282 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2234 2486 2597 2852 3599 3958
Size: 648 1011 57 252 111 255 747 359
Cuts at: 3958 4117 518
Size: 159 2042
Fragments arranged by size:
2042 1011 747 648 359 255 252 159
111 57
MspA1I CmG'CkG
Cuts at: 1606 2500 2696 3057 3734 4882 5127 1606
Size: 894 196 361 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 361 245 196
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2896 2999 4544 1132
Size: 97 1667 103 1545 2229
Fragments arranged by size:
2229 1667 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 2892 4540 2892
Size: 1648 3993
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2696 1108
Size: 1588 4053
PfoI T'CCnGG_A
Cuts at: 2463 2807 2463
Size: 344 5297
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2076 2993 830
Size: 1246 917 3478
Fragments arranged by size:
3478 1246 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2500 2696 2500
Size: 196 5445
SalI G'TCGA_C
Cuts at: 1936 2210 2983 1936
Size: 274 773 4594
Fragments arranged by size:
4594 773 274
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2072 2495 2989 4124 4805 4996 826
Size: 219 1027 423 494 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 494 423 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3745 4646 4908 820
Size: 197 926 615 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2205 3080 513
Size: 552 1140 875 3074
Fragments arranged by size:
3074 1140 875 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 2403 3668 3685 3838 4023 4468
Size: 1255 735 88 1265 17 153 185 445
Cuts at: 4468 325
Size: 1498
Fragments arranged by size:
1498 1265 1255 735 445 185 153 88
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3887 5599
Size: 274 80 33 51 1443 204 1706 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3770 2040
Size: 1730 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI