Restriction map for
pFN21AE5941
(Circular) (Six-base) MAPSORT of: pFN21AE5941.seq Check: 2566 from: 1 to: 5641
pFN21AE5941 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2678 2678
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 2984 1937
Size: 900 147 4594
Fragments arranged by size:
4594 900 147
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 3704 5088 5356 1632
Size: 1070 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1070 1002 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 2966 1870
Size: 73 321 702 4545
Fragments arranged by size:
4545 702 321 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2612 2962 4417 618
Size: 325 217 627 825 350 1455 1842
Fragments arranged by size:
1842 1455 825 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 2960 1391
Size: 1514 55 4072
Fragments arranged by size:
4072 1514 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BbvCI CC'TCA_GC
Cuts at: 2099 2192 2099
Size: 93 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2625 1548
Size: 1077 4564
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2431 2617 3702 4858 1790
Size: 641 186 1085 1156 2573
Fragments arranged by size:
2573 1156 1085 641 186
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 4301 1239
Size: 802 2260 2579
Fragments arranged by size:
2579 2260 802
Bpu10I CC'TnA_GC
Cuts at: 2099 2192 2099
Size: 93 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2072 2741 1186
Size: 847 39 669 4086
Fragments arranged by size:
4086 847 669 39
BseYI C'CCAG_C
Cuts at: 2067 4844 5340 2067
Size: 2777 496 2368
Fragments arranged by size:
2777 2368 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2462 2532 2656 1163
Size: 573 417 309 70 124 4148
Fragments arranged by size:
4148 573 417 309 124 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2431 2617 2905 2960 3702 3787 4858
Size: 399 641 186 288 55 742 85 1071
Cuts at: 4858 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 641 399 288 186 85
55
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2622 3530 5260 2622
Size: 908 1730 3003
Fragments arranged by size:
3003 1730 908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2678 2996 844
Size: 1295 539 318 3489
Fragments arranged by size:
3489 1295 539 318
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3528 1394
Size: 429 187 1518 3507
Fragments arranged by size:
3507 1518 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2769 4136 4310 65
Size: 1183 1521 1367 174 1396
Fragments arranged by size:
1521 1396 1367 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 2519 794
Size: 1691 34 3916
Fragments arranged by size:
3916 1691 34
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2248 2122
Size: 126 5515
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2838 2838
Size: 5641
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 487
Size: 683 56 1330 3572
Fragments arranged by size:
3572 1330 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 2372 4648 809
Size: 507 704 352 2276 1802
Fragments arranged by size:
2276 1802 704 507 352
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2250 3088
Size: 54 1425 50 275 18 282 138 838
Cuts at: 3088 3977 5606 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 838 282 275 138 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3571 1353
Size: 1406 812 3423
Fragments arranged by size:
3423 1406 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2702 3704 4301 5088 5356 1239
Size: 393 409 661 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 661 597 409 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2588 2606 2588
Size: 18 5623
EcoO109I rG'GnC_Cy
Cuts at: 2686 2686
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 4282 4314 4356
Size: 32 135 32 829 32 1849 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1849 829 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2252 10
Size: 54 1425 343 282 138 3399
Fragments arranged by size:
3399 1425 343 282 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2303 2324 2586 2774 3599 3958
Size: 648 1011 126 21 262 188 825 359
Cuts at: 3958 4117 518
Size: 159 2042
Fragments arranged by size:
2042 1011 825 648 359 262 188 159
126 21
MspA1I CmG'CkG
Cuts at: 1606 2807 3057 3734 4882 5127 1606
Size: 1201 250 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 677 250 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2107 2999 4544 1132
Size: 97 878 892 1545 2229
Fragments arranged by size:
2229 1545 892 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2807 2807
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2758 2872 2989 4124 4805 4996 826
Size: 219 1713 114 117 1135 681 191 1471
Fragments arranged by size:
1713 1471 1135 681 219 191 117 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 3745 4646 4908 820
Size: 197 926 321 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 321 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 3080 513
Size: 552 2015 3074
Fragments arranged by size:
3074 2015 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3668 3685 3838 4023 4468 325
Size: 1255 860 1228 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1228 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3887 5599
Size: 274 80 33 51 1443 204 1706 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 5601 5633 2436
Size: 32 3133 32 2444
Fragments arranged by size:
3133 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 2264 1943
Size: 321 5320
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 3770 2714
Size: 1056 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI