Restriction map for
pFN21AE5930
(Circular) (Six-base) MAPSORT of: pFN21AE5930.seq Check: 1411 from: 1 to: 5638
pFN21AE5930 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 2726 2059
Size: 667 4971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2670 3701 5085 5353 1632
Size: 575 463 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 575 463 268
AfeI AGC'GCT
Cuts at: 2112 2112
Size: 5638
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2484 4537 5514 1203
Size: 1281 2053 977 1327
Fragments arranged by size:
2053 1327 1281 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2021 1166
Size: 855 4783
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2074 2228 3381 4953 2074
Size: 154 1153 1572 2759
Fragments arranged by size:
2759 1572 1153 154
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2030 2947 3173 3325 5434 1493
Size: 537 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2609 2766 2957 1391
Size: 1218 157 191 4072
Fragments arranged by size:
4072 1218 191 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2328 2447 3055 2328
Size: 119 608 4911
Fragments arranged by size:
4911 608 119
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
Bpu10I CC'TnA_GC
Cuts at: 2603 2603
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2280 3763 4628 4926 2280
Size: 1483 865 298 2992
Fragments arranged by size:
2992 1483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2727 4841 5337 2727
Size: 2114 496 3028
Fragments arranged by size:
3028 2114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 1163
Size: 573 324 4741
Fragments arranged by size:
4741 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2609 2676 3699 3784 4855 2499
Size: 110 67 1023 85 1071 3282
Fragments arranged by size:
3282 1071 1023 110 85 67
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2609 2676 2766 2957 3699 3784
Size: 399 709 110 67 90 191 742 85
Cuts at: 3784 4855 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 709 399 191 110 90
85 67
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2637 3527 5257 2637
Size: 890 1730 3018
Fragments arranged by size:
3018 1730 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2133 2726 2993 844
Size: 1215 74 593 267 3489
Fragments arranged by size:
3489 1215 593 267 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2133 4133 4307 65
Size: 1183 885 2000 174 1396
Fragments arranged by size:
2000 1396 1183 885 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 794
Size: 1280 4358
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2206 2507 2968 3335 3720 3737 5189 5275
Size: 234 301 461 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 461 385 367 346 301 234
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2776 2776
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603
Size: 54 1425 50 275 18 1255 889 1629
Cuts at: 5603 8
Size: 43
Fragments arranged by size:
1629 1425 1255 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2607 2607
Size: 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2670 3701 4298 5085 5353 1239
Size: 393 575 463 1031 597 787 268 1524
Fragments arranged by size:
1524 1031 787 597 575 463 393 268
EcoRI G'AATT_C
Cuts at: 2030 2947 3325 2030
Size: 917 378 4343
Fragments arranged by size:
4343 917 378
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2114 4785 1678
Size: 436 2671 2531
Fragments arranged by size:
2671 2531 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 2784 3237 2784
Size: 453 5185
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2171 3596 3955 4114 518
Size: 648 855 150 1425 359 159 2042
Fragments arranged by size:
2042 1425 855 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2488 2996 4541 1132
Size: 97 1259 508 1545 2229
Fragments arranged by size:
2229 1545 1259 508 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 2484 4537 2484
Size: 2053 3585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2070 2990 830
Size: 1240 920 3478
Fragments arranged by size:
3478 1240 920
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SacI G_AGCT'C
Cuts at: 2609 2609
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2066 2986 4121 4802 4993 826
Size: 219 1021 920 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 920 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2295 3742 4643 4905 820
Size: 197 926 352 1447 901 262 1553
Fragments arranged by size:
1553 1447 926 901 352 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 3077 513
Size: 552 939 1073 3074
Fragments arranged by size:
3074 1073 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 3665 3682 3835 4020 4465 325
Size: 1255 605 1480 17 153 185 445 1498
Fragments arranged by size:
1498 1480 1255 605 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3884 5596
Size: 274 80 33 51 1443 198 1709 1712
Cuts at: 5596 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 2694 1316
Size: 1378 4260
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2683 3767 2683
Size: 1084 4554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI