Restriction map for
pFN21AE5926
(Circular) (Six-base) MAPSORT of: pFN21AE5926.seq Check: 6920 from: 1 to: 5638
pFN21AE5926 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 3701 5085 5353 1632
Size: 1295 774 1384 268 1917
Fragments arranged by size:
1917 1384 1295 774 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 2260 4367 2260
Size: 2107 3531
AleI CACnn'nnGTG
Cuts at: 1166 2741 1166
Size: 1575 4063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2445 1184
Size: 1261 4377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 2505
Size: 32 5606
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2036 2947 3173 3325 5434 1493
Size: 543 911 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2908 2920 928
Size: 971 1009 12 3646
Fragments arranged by size:
3646 1009 971 12
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2481 5633 2481
Size: 3152 2486
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2763 3699 4855 1790
Size: 973 936 1156 2573
Fragments arranged by size:
2573 1156 973 936
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
Bpu10I CC'TnA_GC
Cuts at: 2678 2678
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3763 4628 4926 2609
Size: 1154 865 298 3321
Fragments arranged by size:
3321 1154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2688 4301 882
Size: 617 1189 1613 2219
Fragments arranged by size:
2219 1613 1189 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2221 2251 1178
Size: 30 165 30 818 30 4565
Fragments arranged by size:
4565 818 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2215 1186
Size: 937 92 4609
Fragments arranged by size:
4609 937 92
BseYI C'CCAG_C
Cuts at: 2122 4841 5337 2122
Size: 2719 496 2423
Fragments arranged by size:
2719 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2498 1163
Size: 573 642 120 4303
Fragments arranged by size:
4303 642 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2763 2957 3699 3784 4855 1391
Size: 399 973 194 742 85 1071 2174
Fragments arranged by size:
2174 1071 973 742 399 194 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2409 3527 5257 2409
Size: 1118 1730 2790
Fragments arranged by size:
2790 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 4133 4307 65
Size: 1183 1181 1704 174 1396
Fragments arranged by size:
1704 1396 1183 1181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 96
Size: 2010 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 2918 3698 4710 2918
Size: 780 1012 3846
Fragments arranged by size:
3846 1012 780
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2758 794
Size: 1964 3674
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 2143 2743 1184
Size: 959 600 4079
Fragments arranged by size:
4079 959 600
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2324 2011
Size: 313 5325
BstYI r'GATC_y
Cuts at: 1972 2481 2968 3335 3720 3737 5189 5275 5287
Size: 509 487 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 509 487 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 487
Size: 683 56 1330 3569
Fragments arranged by size:
3569 1330 683 56
BtsI GCAGTG_nn'
Cuts at: 2427 2790 3154 3948 3968 2427
Size: 363 364 794 20 4097
Fragments arranged by size:
4097 794 364 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2158 2680 4645 809
Size: 507 842 522 1965 1802
Fragments arranged by size:
1965 1802 842 522 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3085 3974
Size: 54 1425 50 275 18 546 709 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 709 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2903 3568 1353
Size: 965 585 665 3423
Fragments arranged by size:
3423 965 665 585
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2927 3701 4298 5085 5353 1239
Size: 393 1295 774 597 787 268 1524
Fragments arranged by size:
1524 1295 787 774 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2554 2554
Size: 5638
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2851 2883 4279
Size: 32 135 32 674 32 573 32 1396
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1396 674 573 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 4752 4936 1305
Size: 1326 2121 184 2007
Fragments arranged by size:
2121 2007 1326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3270
Fragments arranged by size:
3270 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2669 2738 2741 3596 3955 4114
Size: 648 1011 492 69 3 855 359 159
Cuts at: 4114 518
Size: 2042
Fragments arranged by size:
2042 1011 855 648 492 359 159 69
3
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 1108
Size: 969 4669
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 3742 4643 4905 820
Size: 197 926 645 1154 901 262 1553
Fragments arranged by size:
1553 1154 926 901 645 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2323 3077 513
Size: 552 939 319 754 3074
Fragments arranged by size:
3074 939 754 552 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2181 3884
Size: 274 80 33 51 1443 129 75 1703
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 129 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 5598 5630 2221
Size: 32 3345 32 2229
Fragments arranged by size:
3345 2229 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2614 2324
Size: 290 5348
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI