Restriction map for
pFN21AE5923
(Circular) (Six-base) MAPSORT of: pFN21AE5923.seq Check: 3567 from: 1 to: 5638
pFN21AE5923 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2749 2820 2896 3701 5085 5353 1632
Size: 941 176 71 76 805 1384 268 1917
Fragments arranged by size:
1917 1384 941 805 268 176 76 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2475 4537 5514 1203
Size: 1272 2062 977 1327
Fragments arranged by size:
2062 1327 1272 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2837 1166
Size: 1671 3967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2822 3381 4953 2822
Size: 559 1572 3507
Fragments arranged by size:
3507 1572 559
ApaI G_GGCC'C
Cuts at: 2899 2899
Size: 5638
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2030 2085 2947 3173 3325 5434 1493
Size: 537 55 862 226 152 2109 1697
Fragments arranged by size:
2109 1697 862 537 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 2959 4414 618
Size: 325 217 627 822 350 1455 1842
Fragments arranged by size:
1842 1455 822 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2899 2957 1391
Size: 1508 58 4072
Fragments arranged by size:
4072 1508 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BfrBI ATG'CAT
Cuts at: 2637 2637
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2425 2899 3699 4855 1790
Size: 635 474 800 1156 2573
Fragments arranged by size:
2573 1156 800 635 474
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 2520 3369
Size: 416 53 83 399 24 710 393 849
Cuts at: 3369 4327 442
Size: 958 1753
Fragments arranged by size:
1753 958 849 710 416 399 393 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 4298 1239
Size: 804 2255 2579
Fragments arranged by size:
2579 2255 804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2598 2628 1178
Size: 30 165 30 855 30 310 30 4188
Fragments arranged by size:
4188 855 310 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2442 4841 5337 2442
Size: 2399 496 2743
Fragments arranged by size:
2743 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 1163
Size: 573 405 4660
Fragments arranged by size:
4660 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 2899 2957 3699 3784 4855 1391
Size: 399 635 474 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 474 399 85 58
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2403 3527 5257 2403
Size: 1124 1730 2784
Fragments arranged by size:
2784 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2163 2726 2993 844
Size: 1289 30 563 267 3489
Fragments arranged by size:
3489 1289 563 267 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 2811 2832 4133 4307 65
Size: 1183 1542 21 21 1301 174 1396
Fragments arranged by size:
1542 1396 1301 1183 174 21 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2822 794
Size: 2028 3610
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2675 2873 2675
Size: 198 5440
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 2689 513
Size: 552 939 345 340 3462
Fragments arranged by size:
3462 939 552 345 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2187 2370
Size: 54 1425 50 275 18 309 48 183
Cuts at: 2370 3085 3974 5603 8
Size: 715 889 1629 43
Fragments arranged by size:
1629 1425 889 715 309 275 183 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2768 2796 2915 3568 1353
Size: 731 684 28 119 653 3423
Fragments arranged by size:
3423 731 684 653 119 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2573 2749 2820 2896 3701 4298
Size: 393 411 530 176 71 76 805 597
Cuts at: 4298 5085 5353 1239
Size: 787 268 1524
Fragments arranged by size:
1524 805 787 597 530 411 393 268
176 76 71
EcoO109I rG'GnC_Cy
Cuts at: 2409 2446 2757 2895 2896 2409
Size: 37 311 138 1 5151
Fragments arranged by size:
5151 311 138 37 1
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2057 4785 1678
Size: 379 2728 2531
Fragments arranged by size:
2728 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 4279 4311 4353
Size: 32 135 32 676 32 1999 32 42
Cuts at: 4353 4385 1373
Size: 32 2626
Fragments arranged by size:
2626 1999 676 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2982 3228 669
Size: 988 281 268 776 246 3079
Fragments arranged by size:
3079 988 776 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2189 2372 10
Size: 54 1425 343 309 48 183 3276
Fragments arranged by size:
3276 1425 343 309 183 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2297 2402 2837 3596 3955 4114
Size: 648 1005 126 105 435 759 359 159
Cuts at: 4114 518
Size: 2042
Fragments arranged by size:
2042 1005 759 648 435 359 159 126
105
MspA1I CmG'CkG
Cuts at: 1606 2822 3054 3731 4879 5124 1606
Size: 1216 232 677 1148 245 2120
Fragments arranged by size:
2120 1216 1148 677 245 232
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3802
Fragments arranged by size:
3802 939 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NsiI A_TGCA'T
Cuts at: 2639 2639
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 2757 2446
Size: 311 5327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2895 2895
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2723 2990 830
Size: 1893 267 3478
Fragments arranged by size:
3478 1893 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2822 2822
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SanDI GG'GwC_CC
Cuts at: 2757 2757
Size: 5638
SapI GCTCTTCn'nnn_
Cuts at: 2915 2915
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2719 2866 2986 4121 4802 4993 826
Size: 219 1674 147 120 1135 681 191 1471
Fragments arranged by size:
1674 1471 1135 681 219 191 147 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4447
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 3077 513
Size: 552 939 345 728 3074
Fragments arranged by size:
3074 939 728 552 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2588 3665 3682 3835 4020 4465 325
Size: 1255 1008 1077 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1077 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2829 3884
Size: 274 80 33 51 1443 198 654 1055
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1055 654 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 5598 5630 2258
Size: 32 3308 32 2266
Fragments arranged by size:
3308 2266 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2326 2974 2326
Size: 648 4990
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI