Restriction map for
pFN21AE5901
(Circular) (Six-base) MAPSORT of: pFN21AE5901.seq Check: 591 from: 1 to: 5632
pFN21AE5901 5632 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5632
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3695 5079 5347 1632
Size: 1069 994 1384 268 1917
Fragments arranged by size:
1917 1384 1069 994 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 2775 4531 5508 1203
Size: 1572 1756 977 1327
Fragments arranged by size:
1756 1572 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 2843 1166
Size: 1677 3955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 2724 3375 4947 2724
Size: 651 1572 3409
Fragments arranged by size:
3409 1572 651
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2920 2957 1870
Size: 73 977 37 4545
Fragments arranged by size:
4545 977 73 37
BamHI G'GATC_C
Cuts at: 2158 2962 3329 2158
Size: 804 367 4461
Fragments arranged by size:
4461 804 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 2953 4408 618
Size: 325 217 627 975 191 1455 1842
Fragments arranged by size:
1842 1455 975 627 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2330 2951 1391
Size: 939 621 4072
Fragments arranged by size:
4072 939 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 928
Size: 971 380 4281
Fragments arranged by size:
4281 971 380
BbvCI CC'TCA_GC
Cuts at: 2681 2681
Size: 5632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2351 3524 4734 2351
Size: 1173 1210 3249
Fragments arranged by size:
3249 1210 1173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 3693 4849 1790
Size: 1903 1156 2573
Fragments arranged by size:
2573 1903 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 2333 3363
Size: 416 53 83 399 24 754 162 1030
Cuts at: 3363 4321 442
Size: 958 1753
Fragments arranged by size:
1753 1030 958 754 416 399 162 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 4292 1239
Size: 854 2199 2579
Fragments arranged by size:
2579 2199 854
Bpu10I CC'TnA_GC
Cuts at: 2681 2681
Size: 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2655 3757 4622 4920 2543
Size: 112 1102 865 298 3255
Fragments arranged by size:
3255 1102 865 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2776 3423 493
Size: 711 1572 647 2702
Fragments arranged by size:
2702 1572 711 647
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2229 2719 1186
Size: 1043 490 4099
Fragments arranged by size:
4099 1043 490
BseYI C'CCAG_C
Cuts at: 4835 5331 4835
Size: 496 5136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3693 3778 4849 3693
Size: 85 1071 4476
Fragments arranged by size:
4476 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2951 3693 3778 4849 1391
Size: 399 540 621 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 540 399 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2409 3521 5251 2409
Size: 1112 1730 2790
Fragments arranged by size:
2790 1730 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 2790 2987 844
Size: 1888 58 197 3489
Fragments arranged by size:
3489 1888 197 58
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2023 2373 4127 4301 65
Size: 1183 775 350 1754 174 1396
Fragments arranged by size:
1754 1396 1183 775 350 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 2845 1184
Size: 1661 3971
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2158 2962 3329 3714 3731 5183 5269 5281
Size: 186 804 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 804 385 367 346 186 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2417 2841 513
Size: 552 939 413 424 3304
Fragments arranged by size:
3304 939 552 424 413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2859 2936 2859
Size: 77 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 4639 809
Size: 507 1266 2057 1802
Fragments arranged by size:
2057 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597
Size: 54 1425 50 275 18 1249 889 1629
Cuts at: 5597 8
Size: 43
Fragments arranged by size:
1629 1425 1249 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2559 2837 3562 1353
Size: 819 387 278 725 3423
Fragments arranged by size:
3423 819 725 387 278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2701 3695 4292 5079 5347 1239
Size: 393 461 608 994 597 787 268 1524
Fragments arranged by size:
1524 994 787 608 597 461 393 268
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 2976 3222 669
Size: 988 281 316 722 246 3079
Fragments arranged by size:
3079 988 722 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 2254 3222 2254
Size: 968 4664
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 2498 3231 2498
Size: 733 4899
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3590 3949 4108 518
Size: 648 1677 747 359 159 2042
Fragments arranged by size:
2042 1677 747 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2434 3048 3725 4873 5118 1606
Size: 828 614 677 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 614 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2841 513
Size: 552 939 837 3304
Fragments arranged by size:
3304 939 837 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 2524 1108
Size: 1326 90 4216
Fragments arranged by size:
4216 1326 90
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2434 2434
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SapI GCTCTTCn'nnn_
Cuts at: 2559 2559
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2980 4115 4796 4987 826
Size: 219 1935 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2670 3736 4637 4899 820
Size: 197 926 615 112 1066 901 262 1553
Fragments arranged by size:
1553 1066 926 901 615 262 197 112
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2812 2841 3071 513
Size: 552 939 357 451 29 230 3074
Fragments arranged by size:
3074 939 552 451 357 230 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3878 5590
Size: 274 80 33 51 1443 204 1697 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1697 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2845 2877 5592 5624 2845
Size: 32 2715 32 2853
Fragments arranged by size:
2853 2715 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI